Optical recordings of excitability in the human enteric nervous system

Peters, Saskia; Neunlist, Michel; Bischoff, Stephan C.; Schemann, Michael

doi:10.1016/s0016-5085(00)82814-0

Cited by 2 publications

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“…We have developed protocols to use MSORT in freshly dissected preparations from guinea pig, mouse, and human (10,12). The myenteric or submucosal plexus is kept at physiological temperature, is carbogen-gassed perfusate, and is not treated with antioxidants.…”

Section: Spatiotemporal Imaging With Msort and Vsdmentioning

confidence: 99%

III. Imaging and the gastrointestinal tract: mapping the human enteric nervous system

Schemann

Michel

Peters

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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Monitoring membrane potentials by multisite optical recording techniques using voltage-sensitive dyes is ideal for direct analysis of network signaling. We applied this technology to monitor fast and slow excitability changes in the enteric nervous system and in hundreds of neurons simultaneously at cellular and subcellular resolution. This imaging technique presents a powerful tool to study activity patterns in enteric pathways and to assess differential activation of nerves in the gut to a number of stimuli that modulate neuronal activity directly or through synaptic mechanisms. The optical mapping made it possible to record from tissues such as human enteric nerves, which were, until now, inaccessible by other techniques.

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Section: Spatiotemporal Imaging With Msort and Vsdmentioning

confidence: 99%

III. Imaging and the gastrointestinal tract: mapping the human enteric nervous system

Schemann

Michel

Peters

et al. 2002

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Convergence of synaptic input occurred in 65-75%. They also observed different activation patterns with stimulation of different fiber tracts in guinea pig and mice enteric neurons (21,23). Further experiments with increased distances of ETS application should be performed to investigate the contribution of antidromic and synaptic activation.…”

Section: Discussionmentioning

confidence: 95%

“…With the use of the multisite optical recording technique, Peters et al (23) demonstrated induction of fast EPSPs by fiber tract stimulation in 72 Ϯ 12% of human submucosal plexus ganglia. Convergence of synaptic input occurred in 65-75%.…”

Section: Discussionmentioning

confidence: 99%

“…Ascending interneurons, activated by aboral stimulation, connect to excitatory motor neurons, causing a muscle contraction orally, leading to bolus propulsion in the aboral direction. Further support for this concept arose from electrophysiological (10,15,23,25,31,33), immunohistochemical (12,34), and retrograde labeling studies (3,4,24,25,(31)(32)(33) or a combination of these (20) in the gastrointestinal tract of different animal models. In particular, the retrograde labeling experiments established the morphological polarity of projections of myenteric neurons in the guinea pig small intestine (33).…”

mentioning

confidence: 99%

“…Only one study (19) reports simultaneous recordings of electrical responses in different myenteric neurons. In recent years, new techniques have been developed to visualize neuronal activation either by voltage-sensitive dyes (21)(22)(23) or calcium indicators (7,9,27,39,(41)(42)(43)(44)(45). Electrical stimulation of interneuronal fibers of cultured myenteric neurons induced a rise in intracellular calcium concentration ([Ca 2ϩ ] i ), which can be monitored by confocal recording of fluorescence changes in high-affinity calcium indicators, such as fluo 3.…”

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Electrical stimulation reveals complex neuronal input and activation patterns in single myenteric guinea pig ganglia

Bisschops

Berghe

Bellon

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

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The myenteric plexus plays a key role in the control of gastrointestinal motility. We used confocal calcium imaging to study responses to electrical train stimulation (ETS) of interganglionic fiber tracts in entire myenteric ganglia of the guinea pig small intestine. ETS induced calcium transients in a subset of neurons: 52.2% responded to oral ETS, 65.4% to aboral ETS, and 71.7% to simultaneous oral and aboral ETS. A total of 41.3% of the neurons displayed convergence of oral and aboral ETS-induced responses. Responses could be reversibly blocked with TTX (10(-)6 M), demonstrating involvement of neuronal conduction, and by removal of extracellular calcium. omega-Conotoxin (5 x 10(-7) M) blocked the majority of responses and reduced the amplitude of residual responses by 45%, indicating the involvement of N-type calcium channels. Staining for calbindin and calretinin did not reveal different response patterns in these immunohistochemically identified neurons. We conclude that, at least for ETS close to a ganglion, confocal calcium imaging reveals complex oral and aboral input to individual myenteric neurons rather than a polarization in spread of activity.

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Optical recordings of excitability in the human enteric nervous system

Cited by 2 publications

References 0 publications

III. Imaging and the gastrointestinal tract: mapping the human enteric nervous system

III. Imaging and the gastrointestinal tract: mapping the human enteric nervous system

Electrical stimulation reveals complex neuronal input and activation patterns in single myenteric guinea pig ganglia

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