Optical scattering coefficient estimated by optical coherence tomography correlates with collagen content in ovarian tissue

Abstract

Optical scattering coefficient from ex vivo unfixed normal and malignant ovarian tissue was quantitatively extracted by fitting optical coherence tomography (OCT) A-line signals to a single scattering model. 1097 average A-line measurements at a wavelength of 1310 nm were performed at 108 sites obtained from 18 ovaries. The average scattering coefficient obtained from the normal tissue group consisted of 833 measurements from 88 sites was 2.41 mm(-1) (± 0.59), while the average coefficient obtained from the malignant tissue group consisted of 264 measurements from 20 sites was 1.55 mm(-1) (± 0.46). The malignant ovarian tissue showed significant lower scattering than the normal group (p < 0.001). The amount of collagen within OCT imaging depth was analyzed from the tissue histological section stained with Sirius Red. The average collagen area fraction (CAF) obtained from the normal tissue group was 48.4% (± 12.3%), while the average CAF obtained from the malignant tissue group was 11.4% (± 4.7%). A statistical significance of the collagen content was found between the two groups (p < 0.001). These results demonstrated that quantitative measurements of optical scattering coefficient from OCT images could be a potential powerful method for ovarian cancer detection.

Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries

“…However, other OCT studies found that low attenuation is correlated with low collagen content, in the case of agarose scaffolds [39] and ex vivo ovarian tissue [40], which is consistent with our results.…”

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“…The attenuation coefficient has been routinely extracted from OCT measurements based on Beer's law, which is appropriate for low NA measurements. This simple model has proven to be useful in detecting cancerous tissues in skin, bladder and brain [1][2][3], monitoring blood glucose concentration [4], characterizing atherosclerosis plaques [5,6] and correlating collagen content with histological staining [7]. Recently, quantification of the attenuation coefficient has been adopted in brain imaging [8] to follow neurological development in mouse models [9], to detect borders of brain tumors in human specimens [3,10], and to examine the physiological alterations in edema [11].…”

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