Optical Second Harmonic Generation in Biological Systems

Fine, Susan B.; Hansen, W.

doi:10.1364/ao.10.002350

Cited by 175 publications

(115 citation statements)

References 2 publications

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“…Most commonly, polarization-dependent SHG measurements are performed to evaluate two classes of sample properties: (1) What is the local sample orientation given the known nonlinear susceptibility tensors in the local frame? (2) What are the local-frame tensors given the orientation angles?…”

Section: For Collagen Fibersmentioning

confidence: 99%

“…Second harmonic generation (SHG) has a rich history in structural analysis of biological tissues (1)(2)(3)(4)(5), having been used to visualize structures including microtubule assemblies in brain tissue (6) and Caenorhabditis elegans embryos (7), collagen organization in tumors (8), pericardial tissue (9), human atrial myocardium (10), rat tails (11,12), corneas (13), and human skin (14), to cite just a select handful of representative studies. SHG has several properties that make it advantageous in biological imaging.…”

Section: Introductionmentioning

confidence: 99%

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Imaging the Nonlinear Susceptibility Tensor of Collagen by Nonlinear Optical Stokes Ellipsometry

Dow

DeWalt

Sullivan

et al. 2016

Biophysical Journal

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Nonlinear optical Stokes ellipsometric (NOSE) microscopy was demonstrated for the analysis of collagen-rich biological tissues. NOSE is based on polarization-dependent second harmonic generation imaging. NOSE was used to access the molecular-level distribution of collagen fibril orientation relative to the local fiber axis at every position within the field of view. Fibril tilt-angle distribution was investigated by combining the NOSE measurements with ab initio calculations of the predicted molecular nonlinear optical response of a single collagen triple helix. The results were compared with results obtained previously by scanning electron microscopy, nuclear magnetic resonance imaging, and electron tomography. These results were enabled by first measuring the laboratory-frame Jones nonlinear susceptibility tensor, then extending to the local-frame tensor through pixel-by-pixel corrections based on local orientation.

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Section: For Collagen Fibersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Imaging the Nonlinear Susceptibility Tensor of Collagen by Nonlinear Optical Stokes Ellipsometry

Dow

DeWalt

Sullivan

et al. 2016

Biophysical Journal

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“…The 2PF imaging of intrinsic fluorophores contained in the corneal cells and the SHG signal imaging of the stromal collagens can be realized when the photon intensity reaches MW-GW/cm -2 (Fine & Hansen, 1971;So et al, 1998). High photon intensity was induced in the following studies by the ultrashort laser pulses in a sub-femtolitre intrastromal focus volume maintained by the diffraction-limited objectives.…”

Section: Corneal 2-photon Excitation Imagingmentioning

confidence: 99%

Two‐photon microscopy of deep intravital tissues and its merits in clinical research

Wang

König

2010

Journal of Microscopy

180

131

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SummaryMultiphoton excitation laser scanning microscopy, relying on the simultaneous absorption of two or more photons by a molecule, is one of the most exciting recent developments in biomedical imaging. Thanks to its superior imaging capability of deeper tissue penetration and efficient light detection, this system becomes more and more an inspiring tool for intravital bulk tissue imaging. Two-photon excitation microscopy including 2-photon fluorescence and second harmonic generated signal microscopy is the most common multiphoton microscopic application. In the present review we take diverse ocular tissues as intravital samples to demonstrate the advantages of this approach. Experiments with registration of intracellular 2-photon fluorescence and extracellular collagen second harmonic generated signal microscopy in native ocular tissues are focused. Data show that the in-tandem combination of 2-photon fluorescence and second harmonic generated signal microscopy as two-modality microscopy allows for in situ co-localization imaging of various microstructural components in the whole-mount deep intravital tissues. New applications and recent developments of this high technology in clinical studies such as 2-photon-controlled drug release, in vivo drug screening and administration in skin and kidney, as well as its uses in tumourous tissues such as melanoma and glioma, in diseased lung, brain and heart are additionally reviewed. Intrinsic emission two-modal 2-photon microscopy/tomography, acting as an efficient and sensitive non-injurious imaging approach featured by high contrast and subcellular spatial resolution, has been proved to be a promising tool for intravital deep tissue imaging and clinical studies. Given the level of its performance, we believe Correspondence to B.-G. Wang. Tel: +49 3641 938496; fax: +49 3641 938552; e-mail: Baogui.Wang@mti.uni-jena.de that the non-linear optical imaging technique has tremendous potentials to find more applications in biomedical fundamental and clinical research in the near future.

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“…Collagen appears to be the tissue constituent mainly responsible for SHG [18,19]. Collagen molecules consist of three peptide chains that form a rod-shaped triple helix.…”

Section: Mpm and Second Harmonic Generation (Shg) Imagingmentioning

confidence: 99%

“…Several researchers have reported endogenous second harmonic generation in biological tissues. Fine and Hansen [18] used a ruby laser to irradiate a variety of excised tissues. SHG was observed only in collagenous tissues (cornea, sclera, tendon, and skin).…”

Section: Mpm and Second Harmonic Generation (Shg) Imagingmentioning

confidence: 99%

In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy

et al. 2004

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Background and Objective: Current techniques for oral diagnosis require surgical biopsy of lesions, and may fail to detect early malignant change. Non-invasive, sensitive tools providing early detection of oral cancer and a better understanding of malignant change are needed. These studies evaluated in vivo multiphoton excited fluorescence (MPM) techniques to (1) map epithelial and subepithelial changes through out oral carcinogenesis and (2) serve as an effective diagnostic modality. Study Design/Materials and Methods: In the hamster model (n ¼ 70), epithelial and subepithelial change was imaged in vivo throughout carcinogenesis. MPM-and histopathology-based diagnoses on a scale of 0 (healthy)-6 (squamous cell carcinoma [s.c.c.]) were scored by two prestandardized investigators. Results: Collagen matrix and fibers, cellular infiltrates, blood vessels, and microtumors were clearly visible. MPM agreed with the histopathology for 88.6% of diagnoses. Conclusions: In vivo MPM images provide (1) high resolution information on specific components of the carcinogenesis process (2) an excellent basis for oral diagnostics.

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Optical Second Harmonic Generation in Biological Systems

Cited by 175 publications

References 2 publications

Imaging the Nonlinear Susceptibility Tensor of Collagen by Nonlinear Optical Stokes Ellipsometry

Imaging the Nonlinear Susceptibility Tensor of Collagen by Nonlinear Optical Stokes Ellipsometry

Two‐photon microscopy of deep intravital tissues and its merits in clinical research

In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy

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