Optical sensing by integration of analyte-sensitive fluorophore to particles

Carrillo‐Carrión, Carolina; Escudero, Alberto; Parak, Wolfgang J.

doi:10.1016/j.trac.2016.05.001

Cited by 12 publications

(9 citation statements)

References 70 publications

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“…In the last decades, also an increasing number of modularly built nanoparticle (NP)-based sensors has been reported, utilizing, e.g., silica and polymer NPs, lanthanide-based upconversion NPs, and semiconductor quantum dots in combination with organic sensor dyes 4,19,[39][40][41][42][43][44][45][46] . Nanomaterials which have been the most frequently employed for the preparation of nanosensors for bioimaging applications are organic polymeric particles such as biocompatible polystyrene (PS) NPs (PSNPs), and inorganic silica (SiO 2 ) NPs (SiO 2 -NPs) that can be prepared in a variety of sizes with different surface functionalities [47][48][49][50][51] .…”

Section: Dual Color Ph Probes Made From Silica and Polystyrene Nanopa...mentioning

confidence: 99%

Dual color pH probes made from silica and polystyrene nanoparticles and their performance in cell studies

Srivastava

Tavernaro

Scholtz

et al. 2023

Sci Rep

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Ratiometric green–red fluorescent nanosensors for fluorometrically monitoring pH in the acidic range were designed from 80 nm-sized polystyrene (PS) and silica (SiO2) nanoparticles (NPs), red emissive reference dyes, and a green emissive naphthalimide pH probe, analytically and spectroscopically characterized, and compared regarding their sensing performance in aqueous dispersion and in cellular uptake studies. Preparation of these optical probes, which are excitable by 405 nm laser or LED light sources, involved the encapsulation of the pH-inert red-fluorescent dye Nile Red (NR) in the core of self-made carboxylated PSNPs by a simple swelling procedure and the fabrication of rhodamine B (RhB)-stained SiO2-NPs from a silane derivative of pH-insensitive RhB. Subsequently, the custom-made naphthalimide pH probe, that utilizes a protonation-controlled photoinduced electron transfer process, was covalently attached to the carboxylic acid groups at the surface of both types of NPs. Fluorescence microscopy studies with the molecular and nanoscale optical probes and A549 lung cancer cells confirmed the cellular uptake of all probes and their penetration into acidic cell compartments, i.e., the lysosomes, indicated by the switching ON of the green naphthalimide fluorescence. This underlines their suitability for intracellular pH sensing, with the SiO2-based nanosensor revealing the best performance regarding uptake speed and stability.

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Section: Dual Color Ph Probes Made From Silica and Polystyrene Nanopa...mentioning

confidence: 99%

Dual color pH probes made from silica and polystyrene nanoparticles and their performance in cell studies

Srivastava

Tavernaro

Scholtz

et al. 2023

Sci Rep

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“…As Ln-doped NPs do not show an intrinsic or specific luminescence response to most of the analytes (e.g. pH, oxygen, metal ions and biomolecules), the functionalization of their surface with suitable indicator dyes or recognition elements imparts to the NP the selectivity or specific recognition required for most of the sensing applications, especially for biosensing [335]. A huge diversity of recognition elements have been reported, the most commonly used being antibodies [336], DNA [337], aptamers [338][339][340], avidin/biotin pair [341] and polypeptides [342].…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

et al. 2017

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Abstract:Rare earth based nanostructures constitute a type of functional materials widely used and studied in the recent literature. The purpose of this review is to provide a general and comprehensive overview of the current state of the art, with special focus on the commonly employed synthesis methods and functionalization strategies of rare earth based nanoparticles and on their different bioimaging and biosensing applications. The luminescent (including downconversion, upconversion and permanent luminescence) and magnetic properties of rare earth based nanoparticles, as well as their ability to absorb X-rays, will also be explained and connected with their luminescent, magnetic resonance and X-ray computed tomography bioimaging applications, respectively. This review is not only restricted to nanoparticles, and recent advances reported for in other nanostructures containing rare earths, such as metal organic frameworks and lanthanide complexes conjugated with biological structures, will also be commented on.

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“…[ 9 ] In addition, PLMs show inferior targeting ability or insufficient specific recognition ability in complex biological systems, which vastly lowers the selectivity of bioimaging. [ 10 ] Another problem is that PLMs can be captured by spleen, liver and lung, giving rise to toxicity and biological incompatibility in living organisms when applied to bioimaging. [ 11‐12 ] Thus, further improvements are urgently needed to enhance the performance of PLMs in biological systems.…”

Section: Introductionmentioning

confidence: 99%

Surface Modified Persistent Luminescence Probes for Biosensing and Bioimaging: A Review

et al. 2021

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Persistent luminescence materials (PLMs) are a class of unique luminescent materials that can remain luminescent for a few milliseconds to days without constant excitation. By virtue of the super long decay time, PLMs have been extensively explored in biosensing and bioimaging applications to elimin ate autofluorescence interference and improve signal-to-noise ratio in complex samples and tissues. However, nude PLMs often suffer from the poor stability, selectivity, and biocompatibility in biological system and in vivo, which greatly impedes their applications in biomedicine and bioanalysis. Remarkably, surface modification is a viable solution that endows PLMs with span-new features and can make PLMs suitable for organisms by altering PLMs' interaction with biological system. In this review, commonly used strategies for surface modification of PLMs are briefly introduced, and the applications of surface modified PLMs in biosensing and bioimaging as well as their challenges are summarized. Qiang Luo (top left) received his B.S. degree in chemistry from Lanzhou University in 2018. He is currently a master student under the supervision of Prof. Quan Yuan at Hunan University. His research interests include the development of functional nanomaterials for biosensing and bioimaging. Wenjie Wang (top right) received his B.S. degree from North China Electric Power University in 2018. He is currently a master student under the supervision of Prof. Quan Yuan at Hunan University. His research interests include the synthesis of inorganic nanomaterials for biological application. Jie Tan (bottom left) earned her Ph.D. degree from Zhejiang University in 2016. Then she joined the team of Prof. Weihong Tan in Hunan University as a postdoctoral fellow. Currently, she is an associate professor at Hunan University. Her main research interest is the design of chemically modified nucleic acids for biosensing. Quan Yuan (bottom right) received her B.S. degree from Wuhan University and Ph.D. degree from Peking University. Afterwards, she worked as a postdoctoral researcher in the group of Prof. Weihong Tan at University of Florida. Currently, she is a professor in Hunan University. Her research interests are the construction of nucleic acid nanomaterials and related biomedical applications.

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Optical sensing by integration of analyte-sensitive fluorophore to particles

Cited by 12 publications

References 70 publications

Dual color pH probes made from silica and polystyrene nanoparticles and their performance in cell studies

Dual color pH probes made from silica and polystyrene nanoparticles and their performance in cell studies

Rare earth based nanostructured materials: synthesis, functionalization, properties and bioimaging and biosensing applications

Surface Modified Persistent Luminescence Probes for Biosensing and Bioimaging: A Review

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