Optical signature of erythrocytes by light scattering in microfluidic flows

Dannhauser, David; Rossi, Domenico; Causa, Filippo; Memmolo, Pasquale; Fińizio, Andrea; Wriedt, Thomas; Hellmers, Jens; Eremin, Yu. A.; Ferraro, Pietro; Netti, Paolo A.

doi:10.1039/c5lc00525f

Cited by 46 publications

(52 citation statements)

References 38 publications

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“…It has been demonstrated effective for both micro particles [96] and submicron-sized Brownian particles [27] in cylindrical microchannels. It has also been recently utilized by Dannhauser et al [97] to align erythrocytes for a continuous characterization of the morphological property of every individual cell. This viscoelastic focusing is affected by several factors including the fluid rheological properties (Wi), particle blockage ratio (b), flow rate (Re), and channel length etc.…”

Section: Straight Cylindrical Microchannelsmentioning

confidence: 99%

Particle manipulations in non-Newtonian microfluidics: A review

Liu

et al. 2017

Journal of Colloid and Interface Science

249

192

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a b s t r a c tMicrofluidic devices have been widely used since 1990s for diverse manipulations of particles (a general term of beads, cells, vesicles, drops, etc.) in a variety of applications. Compared to the active manipulation via an externally imposed force field, the passive manipulation of particles exploits the flow-induced intrinsic lift and/or drag to control particle motion with several advantages. Along this direction, inertial microfluidics has received tremendous interest in the past decade due to its capability to handle a large volume of samples at a high throughput. This inertial lift-based approach in Newtonian fluids, however, becomes ineffective and even fails for small particles and/or at low flow rates. Recent studies have demonstrated the potential of elastic lift in non-Newtonian fluids for manipulating particles with a much smaller size and over a much wider range of flow rates. The aim of this article is to provide an overview of the various passive manipulations, including focusing, separation, washing and stretching, of particles that have thus far been demonstrated in non-Newtonian microfluidics.

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Section: Straight Cylindrical Microchannelsmentioning

confidence: 99%

Particle manipulations in non-Newtonian microfluidics: A review

Liu

et al. 2017

Journal of Colloid and Interface Science

249

192

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“…However, the combination of our method with microfluidics approaches that allow hydrodynamic (46) or acoustic (47) focusing of a sample stream at velocities suitable for imaging flow cytometry and rapid camera hardware promise a high degree of automation and significant decrease of the hologram acquisition time down to the millisecond range. Primarily, this time consumption raised from the manual selection of single cells and the manual operation of the utilized digital holographic microscope.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Quantitative phase imaging for cell culture quality control

Kastl¹,

Isbach²,

Dirksen

et al. 2017

Cytometry Pt A

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The potential of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for quantification of cell culture quality was explored. Label-free QPI of detached single cells in suspension was performed by Michelson interferometer-based self-interference DHM. Two pancreatic tumor cell lines were chosen as cellular model and analyzed for refractive index, volume, and dry mass under varying culture conditions. Firstly, adequate cell numbers for reliable statistics were identified. Then, to characterize the performance and reproducibility of the method, we compared results from independently repeated measurements and quantified the cellular response to osmolality changes of the cell culture medium. Finally, it was demonstrated that the evaluation of QPI images allows the extraction of absolute cell parameters which are related to cell layer confluence states. In summary, the results show that QPI enables label-free imaging cytometry, which provides novel complementary integral biophysical data sets for sophisticated quantification of cell culture quality with minimized sample preparation. © 2017 International Society for Advancement of Cytometry.

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“…The core of the characterization method is the solution of the inverse light-scattering (ILS) problem, using a parametric shape model of the characterized particle (24,25). Conceptually similar approach, based on LSP measurement combined with fitting theoretical LSPs, has been recently demonstrated for determination of RBC diameters in a microfluidic channel (32). Conceptually similar approach, based on LSP measurement combined with fitting theoretical LSPs, has been recently demonstrated for determination of RBC diameters in a microfluidic channel (32).…”

Section: Introductionmentioning

confidence: 99%

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

et al. 2017

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Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content. The above-mentioned individual RBC characteristics were measured without fluorescent markers and other chemicals by a SFC equipped only with 660 nm laser for RBC illumination and single detector for measurement of angle-resolved light scattering. The distributions over all RBC characteristics were constructed and processed statistically to form the novel 31 RBC indices for 22 donor samples. Our results confirm the possibility of precise, label-free, enhanced morphological analysis of individual intact RBCs with compact single-detector flow cytometer. Detailed characterization of RBCs with high statistics and precision can be used to increase the value of screening examinations and to reveal pathologies accompanied by abnormality of RBC shape. © 2017 International Society for Advancement of Cytometry.

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Optical signature of erythrocytes by light scattering in microfluidic flows

Cited by 46 publications

References 38 publications

Particle manipulations in non-Newtonian microfluidics: A review

Particle manipulations in non-Newtonian microfluidics: A review

Quantitative phase imaging for cell culture quality control

Advanced consumable‐free morphological analysis of intact red blood cells by a compact scanning flow cytometer

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