Optical Study of Cytochrome cM Formation in Synechocystis

Шувалов, В.А.; Allakhverdiev, Suleyman I.; Sakamoto, Atsushi; Malakhov, Michael P.; Murata, Norio

doi:10.1080/15216540118716

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…The transcript level for its paralog petC , however, was also slightly increased in a culture grown at high salinity. Transcript levels for cytM , which encodes a membrane-bound c -type cytochrome (cytochrome c M ; Shuvalov et al, 2001), increased ∼12-fold in cells grown at high salinity. Increases of cytM transcripts in Synechococcus 7002 have also been reported for cells subjected to nutrient limitation or subjected to high irradiation for 1 h (Ludwig and Bryant, 2011, 2012).…”

Section: Resultsmentioning

confidence: 99%

Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions

Ludwig¹,

Bryant²

2012

Front. Microbio.

144

141

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Synechococcus sp. strain PCC 7002 is a unicellular, euryhaline cyanobacterium. It is a model organism for studies of cyanobacterial metabolism and has great potential for biotechnological applications. It exhibits an exceptional tolerance of high-light irradiation and shows very rapid growth. The habitats from which this and closely related strains were isolated are subject to changes in several environmental factors, including light, nutrient supply, temperature, and salinity. In this study global transcriptome profiling via RNAseq has been used to perform a comparative and integrated study of global changes in cells grown at different temperatures, at different salinities, and under mixotrophic conditions, when a metabolizable organic carbon source was present. Furthermore, the transcriptomes were investigated for cells that were subjected to a heat shock and that were exposed to oxidative stress. Lower growth temperatures caused relatively minor changes of the transcriptome; the most prominent changes affected fatty acid desaturases. A heat shock caused severe changes of the transcriptome pattern; transcripts for genes associated with major metabolic pathways declined and those for different chaperones increased dramatically. Oxidative stress, however, left the transcript pattern almost unaffected. When grown at high salinity, Synechococcus sp. PCC 7002 had increased expression of genes involved in compatible solute biosynthesis and showed increased mRNA levels for several genes involved in electron transport. Transcripts of two adjacent genes dramatically increased upon growth at high salinity; the respective proteins are putatively involved in coping with oxidative stress and in triggering ion channels. Only minor changes were observed when cells were grown at low salinity or when the growth medium was supplemented with glycerol. However, the transcriptome data suggest that cells must acclimate to excess reducing equivalents when a reduced C-source is present.

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Section: Resultsmentioning

confidence: 99%

Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions

Ludwig¹,

Bryant²

2012

Front. Microbio.

144

141

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“…Transcript levels for these genes, however, were largely unaffected under the nutrient limitation conditions tested here (see Text S3 in Supplementary Material). Although its role in electron transfer is not completely clear (Shuvalov et al, 2001), cytochrome c M is a membrane-associated c -type cytochrome, whose expression is enhanced under low-temperature and high-light stress in Synechocystis sp. PCC 6803 (Malakhov et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Acclimation of the Global Transcriptome of the Cyanobacterium Synechococcus sp. Strain PCC 7002 to Nutrient Limitations and Different Nitrogen Sources

Ludwig¹,

Bryant²

2012

Front. Microbio.

126

129

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The unicellular, euryhaline cyanobacterium Synechococcus sp. strain PCC 7002 is a model organism for laboratory-based studies of cyanobacterial metabolism and is a potential platform for biotechnological applications. Two of its most notable properties are its exceptional tolerance of high-light intensity and very rapid growth under optimal conditions. In this study, transcription profiling by RNAseq has been used to perform an integrated study of global changes in transcript levels in cells subjected to limitation for the major nutrients CO2, nitrogen, sulfate, phosphate, and iron. Transcriptional patterns for cells grown on nitrate, ammonia, and urea were also studied. Nutrient limitation caused strong decreases of transcript levels of the genes encoding major metabolic pathways, especially for components of the photosynthetic apparatus, CO2 fixation, and protein biosynthesis. Uptake mechanisms for the respective nutrients were strongly up-regulated. The transcription data further suggest that major changes in the composition of the NADH dehydrogenase complex occur upon nutrient limitation. Transcripts for flavoproteins increased strongly when CO2 was limiting. Genes involved in protection from oxidative stress generally showed high, constitutive transcript levels, which possibly explains the high-light tolerance of this organism. The transcriptomes of cells grown with ammonia or urea as nitrogen source showed increased transcript levels for components of the CO2 fixation machinery compared to cells grown with nitrate, but in general transcription differences in cells grown on different N-sources exhibited surprisingly minor differences.

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In memory of Vladimir Anatolievich Shuvalov (1943–2022): an outstanding biophysicist

et al. 2022

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Optical Study of Cytochrome cM Formation in Synechocystis

Abstract: SummaryThe

Cited by 4 publications

References 15 publications

Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions

Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions

Acclimation of the Global Transcriptome of the Cyanobacterium Synechococcus sp. Strain PCC 7002 to Nutrient Limitations and Different Nitrogen Sources

In memory of Vladimir Anatolievich Shuvalov (1943–2022): an outstanding biophysicist

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