Optical tweezers beyond refractive index mismatch using highly doped upconversion nanoparticles

Shan, Xuchen; Wang, Fan; Wang, Dejiang; Wen, Shihui; Chen, Chaohao; Di, Xiangjun; Nie, Peng; Liao, Jiayan; Liu, Yongtao; Ding, Lei; Reece, Peter J.; Jin, Dayong

doi:10.1038/s41565-021-00852-0

Cited by 108 publications

(81 citation statements)

References 38 publications

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“…This is limited by laser power and the consequential damage threshold of soft biological material and the refractive index differences, which are normally not larger than 0.1 or 0.3 44 . Several methods have been proposed to increase the force detection limit, e.g., using structured light 75 , anti-reflective coated microspheres 76 , high-refractive index particles 77 or highly doped quantum dots 78 .…”

Section: Limitations Of the Technique And Suggestions To Overcome Themmentioning

confidence: 99%

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Català-Castro

Venturini

Ortiz-Vásquez

et al. 2021

JoVE

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During the development of a multicellular organism, a single fertilized cell divides and gives rise to multiple tissues with diverse functions. Tissue morphogenesis goes in hand with molecular and structural changes at the single cell level that result in variations of subcellular mechanical properties. As a consequence, even within the same cell, different organelles and compartments resist differently to mechanical stresses; and mechanotransduction pathways can actively regulate their mechanical properties. The ability of a cell to adapt to the microenvironment of the tissue niche thus is in part due to the ability to sense and respond to mechanical stresses. We recently proposed a new mechanosensation paradigm in which nuclear deformation and positioning enables a cell to gauge the physical 3D environment and endows the cell with a sense of proprioception to decode changes in cell shape. In this article, we describe a new method to measure the forces and material properties that shape the cell nucleus inside living cells, exemplified on adherent cells and mechanically confined cells. The measurements can be performed non-invasively with optical traps inside cells, and the forces are directly accessible through calibration-free detection of light momentum. This allows measuring the mechanics of the nucleus independently from cell surface deformations and allowing dissection of exteroceptive and interoceptive mechanotransduction pathways. Importantly, the trapping experiment can be combined with optical microscopy to investigate the cellular response and subcellular dynamics using fluorescence imaging of the cytoskeleton, calcium ions, or nuclear morphology. The presented method is straightforward to apply, compatible with commercial solutions for force measurements, and can easily be extended to investigate the mechanics of other subcellular compartments, e.g., mitochondria, stress-fibers, and endosomes.

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Section: Limitations Of the Technique And Suggestions To Overcome Themmentioning

confidence: 99%

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Català-Castro

Venturini

Ortiz-Vásquez

et al. 2021

JoVE

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“…Therefore, it is an effective method to improve optical force by modifying the nanoparticle surface [ 58 , 70 ] and/or the surrounding medium [ 71 , 72 , 73 ]. Additionally, extensive doping with lanthanide ions in up-converting nanoparticles is also a good route to enhance the permittivity and polarizability of nanocrystals, leading to enhanced optical trapping forces by several orders of magnitude [ 74 ].…”

Section: Temperature Effect On Optical Trappingmentioning

confidence: 99%

Temperature Effects on Optical Trapping Stability

Gámez

Haro‐González

2021

Micromachines

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In recent years, optically trapped luminescent particles have emerged as a reliable probe for contactless thermal sensing because of the dependence of their luminescence on environmental conditions. Although the temperature effect in the optical trapping stability has not always been the object of study, the optical trapping of micro/nanoparticles above room temperature is hindered by disturbances caused by temperature increments of even a few degrees in the Brownian motion that may lead to the release of the particle from the trap. In this report, we summarize recent experimental results on thermal sensing experiments in which micro/nanoparticles are used as probes with the aim of providing the contemporary state of the art about temperature effects in the stability of potential trapping processes.

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“…[ 35,36 ] It also presents interesting optical properties. NaYF 4 refractive index is 1.46, [ 37,38 ] which leads to a relevant index contrast (Δ n ) when suspended, for instance, in water. This makes optical manipulation of NaYF 4 : Ln micro/nanoparticles feasible.…”

Section: Introductionmentioning

confidence: 99%

Laser Refrigeration by an Ytterbium‐Doped NaYF₄ Microspinner

Ortiz‐Rivero

Prorok

Martı́n

et al. 2021

Small

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Thermal control of liquids with high (micrometric) spatial resolution is required for advanced research such as single molecule/cell studies (where temperature is a key factor) or for the development of advanced microfluidic devices (based on the creation of thermal gradients at the microscale). Local and remote heating of liquids is easily achieved by focusing a laser beam with wavelength adjusted to absorption bands of the liquid medium or of the embedded colloidal absorbers. The opposite effect, that is highly localized cooling, is much more difficult to achieve. It requires the use of a refrigerating micro‐/nanoparticle which should overcome the intrinsic liquid heating. Remote monitoring of such localized cooling, typically of a few degrees, is even more challenging. In this work, a solution to both problems is provided. Remote cooling in D2O is achieved via anti‐Stokes emission by using an optically driven ytterbium‐doped NaYF4 microparticle. Simultaneously, the magnitude of cooling is determined by mechanical thermometry based on the analysis of the spinning dynamics of the same NaYF4 microparticle. The angular deceleration of the NaYF4 particle, caused by the cooling‐induced increase of medium viscosity, reveals liquid refrigeration by over −6 K below ambient conditions.

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Optical tweezers beyond refractive index mismatch using highly doped upconversion nanoparticles

Cited by 108 publications

References 38 publications

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Temperature Effects on Optical Trapping Stability

Laser Refrigeration by an Ytterbium‐Doped NaYF₄ Microspinner

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Optical tweezers beyond refractive index mismatch using highly doped upconversion nanoparticles

Cited by 108 publications

References 38 publications

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Temperature Effects on Optical Trapping Stability

Laser Refrigeration by an Ytterbium‐Doped NaYF4 Microspinner

Contact Info

Product

Resources

About

Laser Refrigeration by an Ytterbium‐Doped NaYF₄ Microspinner