Optically Trapped Surface-Enhanced Raman Probes Prepared by Silver Photoreduction to 3D Microstructures

Vizsnyiczai, Gaszton; Lestyán, Tamás; Joniová, Jaroslava; Aekbote, Badri L.; Strejčková, Alena; Ormos, Pál; Miškovský, Pavol; Kelemen, Lóránd; Bánó, Gregor

doi:10.1021/acs.langmuir.5b01210

Cited by 19 publications

(13 citation statements)

References 48 publications

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“…1 b). Details of the optical setup were given in our previous works (21,22). The trapping laser was operated at 785 nm (10 mW) while another 488 nm (2 mW) laser was used to excite the fluorescence signal.…”

Section: Laser Tweezers Measurementsmentioning

confidence: 99%

Formation of Large Hypericin Aggregates in Giant Unilamellar Vesicles—Experiments and Modeling

Joniová

Rebič

Strejčková

et al. 2017

Biophysical Journal

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The incorporation of hypericin (Hyp) from aqueous solutions into giant unilamellar vesicle (GUV) membranes has been studied experimentally and by means of kinetic Monte Carlo modeling. The time evolution of Hyp fluorescence originating from Hyp monomers dissolved in the GUV membrane has been recorded by confocal microscopy and while trapping individual GUVs in optical tweezers. It was shown that after reaching a maximum, the fluorescence intensity gradually decreased toward longer times. Formation of oversized Hyp clusters has been observed on the GUV surface at prolonged time. A simplified kinetic Monte Carlo model is presented to follow the aggregation/dissociation processes of Hyp molecules in the membrane. The simulation results reproduced the basic experimental observations: the scaling of the characteristic fluorescence decay time with the vesicle diameter and the buildup of large Hyp clusters in the GUV membrane.

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Section: Laser Tweezers Measurementsmentioning

confidence: 99%

Formation of Large Hypericin Aggregates in Giant Unilamellar Vesicles—Experiments and Modeling

Joniová

Rebič

Strejčková

et al. 2017

Biophysical Journal

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“…The parallel polymerization scheme enabled us to routinely fabricate about 1500 structures overnight, generally enough for a dozen of samples. The application of similar two-photon polymerized trapped structures has been published already by others 19,45 and by us 20,46,47 .…”

Section: Microtool Designmentioning

confidence: 78%

Multiview microscopy of single cells through microstructure-based indirect optical manipulation

Vizsnyiczai¹,

Búzás²,

Aekbote³

et al. 2020

Biomed. Opt. Express

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Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several times worse than the transversal. The optical solutions to this problem usually require complicated optics and extreme spatial stability. A straightforward way to eliminate anisotropic resolution is to fuse images recorded from multiple viewing directions achieved mostly by the mechanical rotation of the entire sample. In the presented approach, multiview imaging of single cells is implemented by rotating them around an axis perpendicular to the optical axis by means of holographic optical tweezers. For this, the cells are indirectly trapped and manipulated with special microtools made with two-photon polymerization. The cell is firmly attached to the microtool and is precisely manipulated with 6 degrees of freedom. The total control over the cells position allows for its multiview fluorescence imaging from arbitrarily selected directions. The image stacks obtained this way are combined into one 3D image array with a special image processing algorithm resulting in isotropic optical resolution. The presented tool and manipulation scheme can be readily applied in various microscope platforms.

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“…10549 105491D-3 Light in* anywhere else and was also shown to serve as an endoscope to detect signal in tiny areas of a specimen. Different sensing capabilities of micro-robots have already been demonstrated by other groups with the use of optically trapped spheres and designed micro-structures for Raman sensing [11] and surface imaging [12], among many others, that have resolutions comparable to present techniques. Currently, we are working towards the fabrication of structures that look very similar with the free-standing waveguide, but with a metal-coated tip to possibly enhance Raman signal for particle characterization.…”

Section: Recent Developments In the Field Have Led In Optical Forces mentioning

confidence: 88%

Light robotics: a new field of research

Engay

Chouliara

Bañas

et al. 2018

Complex Light and Optical Forces XII

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After years of working on light-driven trapping and manipulation, we can see that a confluence of developments is now ripe for the emergence of a new area that can contribute to nanobiophotonics-Light Robotics-which combines advances in microfabrication and optical micromanipulation together with intelligent control ideas from robotics, wavefront engineering and information optics. In the Summer 2017 we are publishing a 482 pages edited Elsevier book volume covering the fundamental aspects needed for Light Robotics including optical trapping systems, microfabrication and microassembly as well as underlying theoretical principles and experimental illustrations for optimizing optical forces and torques for Light Robotics.

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Optically Trapped Surface-Enhanced Raman Probes Prepared by Silver Photoreduction to 3D Microstructures

Cited by 19 publications

References 48 publications

Formation of Large Hypericin Aggregates in Giant Unilamellar Vesicles—Experiments and Modeling

Formation of Large Hypericin Aggregates in Giant Unilamellar Vesicles—Experiments and Modeling

Multiview microscopy of single cells through microstructure-based indirect optical manipulation

Light robotics: a new field of research

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