Optimal Conditions and Comparative Effectiveness of Dimethyl Sulphoxide and Polyvinylpyrrolidone in Preservation of Bone Marrow

The Use of Dimethyl Sulphoxide, Glycerol, and Reconstituted Skim Milk for the Preservation of Ram Spermatozoa II. The Influence of Diluent Composition and Processing Time During Freezing to ?79°C with Dimethyl Sulphoxide or Glycerol or Both Compounds

“…Jones (1965) found that during incubation at 5°C, dimethyl sulphoxide was much more toxic to spermatozoa than glycerol so that the protective action of dimethyl sulphoxide during freezing could be masked by its toxicity to spermatozoa. Thus, in these studies, 6% (vjv) dimethyl sulphoxide gave the highest revival rate, whilst with bone marrow cells 10% Richards 1963) and15% (AshwoodSmith 1961b) was used, and with erythrocytes 10-15% was best (Lovelock and Bishop 1959). The short equilibration time (10 min) necessary before fr eezing bone marrow cells in dimethyl sulphoxide (Persidsky and Richards 1963) may indicate a degree of toxicity to these cells.…”

“…Thus, in these studies, 6% (vjv) dimethyl sulphoxide gave the highest revival rate, whilst with bone marrow cells 10% Richards 1963) and15% (AshwoodSmith 1961b) was used, and with erythrocytes 10-15% was best (Lovelock and Bishop 1959). The short equilibration time (10 min) necessary before fr eezing bone marrow cells in dimethyl sulphoxide (Persidsky and Richards 1963) may indicate a degree of toxicity to these cells. However, the combination of a very short equilibration in dimethyl sulphoxide and resuspension in a diluent free of dimethyl sulphoxide after thawing did not markedly increase revival in these expeirments.…”

“…the protective action of dimethyl sulphoxide during freezing have used reticuloendothelial cells as the test material-either the undifferentiated heterogeneous cells in the bone marrow (Ashwood-Smith 1961a, 1961bPersidsky and Richards 1963) or mature myeloid cells, i.e. erythrocytes (Lovelock and Bishop 1959), which are not nucleated.…”

The Use of Dimethyl Sulphoxide, Glycerol, and Reconstituted Skim Milk For The Preservation of Ram Spermatozoa I. The tonicity and toxicity of Dimethyl Sulphoxide and Reconstituted Skim Milk At 30 and 5°C

“…Previous reports (Ashwood-Smith 1961a; Persidsky and Richards 1963) indicate that 10-15% (vjv) dimethyl sulphoxide is needed in a freezing diluent. On a molecular basis this could constitute more than a four-fold increase in tonicity, and unless dimethyl sulphoxide freely permeated spermatozoa, they would not survive in such a hypertonic diluent.…”

“…Lovelock and Bishop (1959) reported that dimethyl sulphoxide was superior to glycerol in protecting erythrocytes, but inferior when used for freezing bull spermatozoa. Ashwood-Smith (1961a, 1961b and Persidsky and Richards (1963) respectively found dimethyl sulphoxide equal to and better than glycerol for freezing bone marrow cells. Other workers have used dimethyl sulphoxide to freeze several strains of human cervical carcinoma (HeLa), chicken embryo cells, human and monkey embryonic kidney cells, lung cells (Dougherty 1962;Porterfield and Ashwood-Smith 1962;Greaves, Nagington, and Kellaway 1963), and trypanosomes (Walker and Ashwood-Smith 1961).…”