Optimal Conditions for Streptococcus pneumoniae Culture and for Polysaccharide Production for Vaccines

Suárez, Norma

doi:10.4172/0974-8369.1000321

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…Despite the common knowledge and the regular growth in exponential phase (31,48), a phase of significant killing after 8-48 hours of incubation was unavoidable in the commonly used growth media Brain Heart Infusion broth (BHI) and Todd-Hewitt broth supplemented with 0.5% Yeast extract (THY), with or without applying the different strategies to counteract pyruvate oxidase or autolysin (supplementary data figure 1). While a section of the stationary phase was reasonably stable (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) fold reduction in bacterial concentration from 16 to 24h) when adding catalase or using a lyta knockout mutant, the bacterial concentration was nevertheless reduced up to 1,000 fold before reaching such stable period.…”

Section: Specific Growth Conditions Allow Long-living In Vitro Cultur...mentioning

confidence: 99%

Antibiotic-tolerant persisters are pervasive among clinical Streptococcus pneumoniae isolates and show strong condition-dependence

Geerts¹,

Vooght²,

Passaris

et al. 2022

Preprint

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Streptococcus pneumoniae is an important human pathogen, being one of the most common causes of community-acquired pneumonia and otitis media. Antibiotic resistance in S. pneumoniae is an emerging problem as it depletes our arsenal of effective drugs. In addition, persistence also contributes to the antibiotic crisis in many other pathogens, yet, in S. pneumoniae nothing is known about antibiotic-tolerant persisters. Persister cells are phenotypic variants that exist as a subpopulation within a clonal culture. Being tolerant to lethal antibiotics, they underly the chronic nature of a variety of infections and even help in acquiring genetic resistance. Here, we set out to identify and characterize persistence in S. pneumoniae. Specifically, we followed different strategies to overcome the self-limiting nature of S. pneumoniae as confounding factor in the prolonged monitoring of antibiotic survival needed to study persistence. In optimized conditions, we identified genuine persisters in various growth phases and for four relevant antibiotics through biphasic survival dynamics and heritability assays. Finally, we detected a high variety in antibiotic survival levels across a diverse collection of S. pneumoniae clinical isolates, which shows that a high natural diversity in persistence is widely present in S. pneumoniae. Collectively, this proof-of-concept significantly progresses the understanding of the importance of antibiotic persistence in S. pneumoniae infections which will set stage for characterizing its relevance to clinical outcomes and advocates for increased attention to the phenotype in both fundamental and clinical research.

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Section: Specific Growth Conditions Allow Long-living In Vitro Cultur...mentioning

confidence: 99%

Antibiotic-tolerant persisters are pervasive among clinical Streptococcus pneumoniae isolates and show strong condition-dependence

Geerts¹,

Vooght²,

Passaris

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media

Suárez

Texeira

2019

Methods in Molecular Biology

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Optimal Conditions for Streptococcus pneumoniae Culture and for Polysaccharide Production for Vaccines

Cited by 2 publications

References 22 publications

Antibiotic-tolerant persisters are pervasive among clinical Streptococcus pneumoniae isolates and show strong condition-dependence

Antibiotic-tolerant persisters are pervasive among clinical Streptococcus pneumoniae isolates and show strong condition-dependence

Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media

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