Optimal conditions for the induction of triploidy in the sea bass (Dicentrarchus labrax L.)

Felip, Alicia; Zanuy, Silvia; Carrillo, Manuel; Martínez, Gonzalo; Ramos, Jesús; Piferrer, Francesc

doi:10.1016/s0044-8486(96)01509-8

Cited by 71 publications

(42 citation statements)

References 18 publications

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“…After hatching and egg yolk absorption, the fish were cultured for three months when they attained a weight range of 50-150g. Erythrocyte observation of the dry blood smear was done to confirm the ploidy characteristics of the fish as described by Felip et al, (1997);Felip et al, (2001) and Normala et al, (2016). Blood was obtained by cutting the caudal fin using a pair of surgical scissors without sacrificing the fish.…”

Section: Methodsmentioning

confidence: 99%

“…Hence, the urgent need to develop management techniques for triploid African catfishes. Generally, methods for the identification and characterization of triploids includes the use of chromosome karyotyping, flow cytometry, microfluorometry, Nucleolar Organizer Region (NOR) as well as erythrocyte measurement (Beaumont and Kelly, 1989;Manickam, 1991;Felip et al, 1997;Piferrer et al, 2000;Gheyas et al, 2001;Karami et al, 2010;Normala et al, 2016). The need to continuously sacrifice fish coupled with the continuous handling of cytotoxic chemical (colchicines) makes the routine applicability of chromosome karyotyping impossible for large scale production (Child and Watkins, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Morphometric Variations Between Triploid and DiploidClarias gariepinus(Burchell, 1822)

Normala

Mohd

Abol

et al. 2017

Croatian Journal of Fisheries

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Several scientific methods have been described in the identification of triploid fish. However, many of these methods are not applicable for routine management purposes due to their complexity and cost. In this study, the possibility of using morphological variation as a least cost and less complex method of distinguishing triploid and diploid African catfish Clarias gariepinus (Burchell, 1822) was examined. Triploid catfish were produced by cold shock of fertilized eggs in 5°C for 20 mins (at approximately 3 mins after fertilization). The fish were incubated, hatched and raised for 3 months. Ploidy levels of the fish were then ascertained by observing the erythrocyte shape. Triploid erythrocyte was ellipsoidal in shape while diploid was round. Morphological characterization was then carried out on 100 samples each of triploid and diploid African catfish. Although significant differences were observed in many parameters, the principal morphometric difference between triploid and diploid African catfish could not be clearly distinguished. It was therefore concluded that morphological characteristics is not ideal for discriminating triploids and diploids of African catfish. The used of erythrocyte characteristics still remains the cheapest and relatively effective method for triploid and diploid determination in African catfish.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphometric Variations Between Triploid and DiploidClarias gariepinus(Burchell, 1822)

Normala

Mohd

Abol

et al. 2017

Croatian Journal of Fisheries

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“…The major variables influencing the effectiveness of pressure shock in inducing triploidy are the following, in order of decreasing importance: timing; intensity; and duration of shock (Felip et al, 1997). Although method optimization for effective induction varies by species (Piferrer et al, 2009), laboratory-scale efficiencies of 100% that have been reported for Atlantic salmon (Benfey & Sutterlin, 1984) are not likely to be attained on a commercial scale (McGeachey et al, 1995).…”

Section: Reliability Of Inducing Triploidy In Aquadvantage Salmon Eggsmentioning

confidence: 99%

Transgenic Salmon: Science, Politics, and Flawed Policy

Bailey

2015

Society & Natural Resources

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“…However, the lack of a systematic control of brood stock, recessive growth due to inbreeding, and the increased male ratio are causing the overall productivity of RT to slump (Hwang 2012). In the global aquaculture industry, the induction of numerous artificial triploid fish species is already an important subject of study (Felip et al 1997;Gjedrem et al 2012;Maxime 2008). The usage of triploid fish for industrial purposes has numerous advantages as it contains three sets of chromosomes and is genetically sterile.…”

Section: Introductionmentioning

confidence: 99%

Comparison of different ploidy detection methods in Oncorhynchus mykiss, the rainbow trout

Kim¹,

Chung

Son³

2017

Fish Aquatic Sci

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The objective of this study was to determine a simple and reliable ploidy identification protocol for the rainbow trout (RT), Oncorhynchus mykiss, in the field condition. To evaluate the ploidy level and compare different detection protocols, triploid RT and gynogenesis were induced by UV irradiation and/or heat shock. The hatching rate at day 30 was 85.2% and the survival rate at day 90 was 69.4% (fingerling). The sex ratio of female RT was 93.75% in the gynogenesis group, illustrating that the UV irradiation inactivated the sperm DNA. The hatching rate and survival rate were 82.0 and 74.7%, respectively, in the triploid-induced group. The triploid induction rate by heat shock procedure was 73.9%. Cytogenetic protocols for ploidy identification such as chromosome counting, erythrocyte nuclear size comparison, and analysis of nucleolar organizing regions (NORs) by silver staining were compared. Silver nitrate staining showed the greatest success rate (22/23 and 32/32 for the triploid-induced group and gynogenesis group, respectively), followed by erythrocyte nuclear size comparison (16/23 and 19/32 for the triploid-induced group and gynogenesis group, respectively) and, lastly, chromosome preparation (2/23 and 6/32 for the triploid-induced group and gynogenesis group, respectively) with the lowest success rate. Based on our findings, silver staining for RT ploidy identification is speculated to be highly applicable in a wide range of research conditions, due to its cost-effectiveness and simplicity compared to other numerous ploidy detection protocols.

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Optimal conditions for the induction of triploidy in the sea bass (Dicentrarchus labrax L.)

Cited by 71 publications

References 18 publications

Morphometric Variations Between Triploid and DiploidClarias gariepinus(Burchell, 1822)

Morphometric Variations Between Triploid and DiploidClarias gariepinus(Burchell, 1822)

Transgenic Salmon: Science, Politics, and Flawed Policy

Comparison of different ploidy detection methods in Oncorhynchus mykiss, the rainbow trout

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