Optimal design of upstream processes in biotransformation technologies

et al. 2020

Biotechnol Biofuels

Background: Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implementation.Results: Succinic acid production has been evaluated using hydrolysates from the organic fraction of municipal solid waste (OFMSW) collected from MSW treatment plants. A tailor-made enzymatic cocktail was used for OFMSW hydrolysate production containing up to 107.3 g/L carbon sources and up to 638.7 mg/L free amino nitrogen. The bacterial strains Actinobacillus succinogenes and Basfia succiniciproducens were evaluated for succinic acid production with the latter strain being less efficient due to high lactic acid production. Batch A. succinogenes cultures supplemented with 5 g/L yeast extract and 5 g/L MgCO 3 reached 29.4 g/L succinic acid with productivity of 0.89 g/L/h and yield of 0.56 g/g. Continuous cultures at dilution rate of 0.06 h −1 reached 21.2 g/L succinic acid with yield of 0.47 g/g and productivity of 1.27 g/L/h. Downstream separation and purification of succinic acid was achieved by centrifugation, treatment with activated carbon, acidification with cation exchange resins, evaporation and drying, reaching more than 99% purity. Preliminary techno-economic evaluation has been employed to evaluate the profitability potential of bio-based succinic acid production. Conclusions:The use of OFMSW hydrolysate in continuous cultures could lead to a minimum selling price of 2.5 $/ kg at annual production capacity of 40,000 t succinic acid and OFMSW hydrolysate production cost of 25 $/t sugars.

Section: Process Feasibility Evaluationmentioning

confidence: 99%

Section: Preliminary Techno-economic Evaluationmentioning

confidence: 99%

Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production

et al. 2020

Biotechnol Biofuels

“…The fermentation stage including sterilization and the DSP have been considered for the estimation of the production cost. The total number of bioreactors required per batch cycle, the volume of each bioreactor and the minimum bioreactor cost were estimated based on the study of Koutinas et al [50] and Dheskali et al [42] using the optimal succinic acid production efficiencies in batch and continuous cultures. The DSP used in this study has been presented by Alexandri et al [51].…”

Section: Preliminary Techno-economic Evaluationmentioning

confidence: 99%

“…Fed-batch fermentations were not used in the technoeconomic evaluation due to the low productivity achieved.The design and costing methodology were applied on the fermentation stage including media sterilization and the downstream separation and purification (DSP) stage that included centrifugation, activated carbon treatment, cation exchange resin treatment, evaporation, crystallization and drying unit operations. The estimation of the optimal design of the fermentation stage, the optimal scheduling of unit operations and the cost estimation of unit operations is based on the work presented by Dheskali et al[42]. The cost of manufacture for succinic acid did not consider the upstream stage of OFMSW pretreatment and hydrolysis.…”

mentioning

confidence: 99%

Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production

et al. 2020

Preprint

Background: Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implementation. Results: Succinic acid production has been evaluated using hydrolysates from the organic fraction of municipal solid waste (OFMSW) collected from MSW treatment plants. A tailor-made enzymatic cocktail was used for OFMSW hydrolysate production containing up to 107.3 g/L carbon sources and up to 638.7 mg/L free amino nitrogen. The bacterial strains Actinobacillus succinogenes and Basfia succiniciproducens were evaluated for succinic acid production with the latter strain being less efficient due to high lactic acid production. Batch A. succinogenes cultures supplemented with 5 g/L yeast extract and 5 g/L MgCO3 reached 29.4 g/L succinic acid with productivity of 0.89 g/L/h and yield of 0.56 g/g. Continuous cultures at dilution rate of 0.06 h -1 reached 21.2 g/L succinic acid with yield of 0.47 g/g and productivity of 1.27 g/L/h. Downstream separation and purification of succinic acid was achieved by centrifugation, treatment with activated carbon, acidification with cation exchange resins, evaporation and drying, reaching more than 99% purity. Preliminary techno-economic evaluation has been employed to evaluate the profitability potential of bio-based succinic acid production. Conclusions: The use of OFMSW hydrolysate in continuous cultures could lead to a minimum selling price of 2.5 $/kg at annual production capacity of 40,000 t succinic acid and OFMSW hydrolysate production cost of 25 $/ t sugars.

“…Succinic acid purification using this method resulted in succinic acid product purity of more than 99% and yield of 95%. The estimation of the optimal design of the fermentation stage, the optimal scheduling of unit operations and the cost estimation of unit operations is based on the work presented by Dheskali et al [42]. The cost of manufacture for succinic acid did not consider the upstream stage of OFMSW pretreatment and hydrolysis.…”

Section: Process Feasibility Evaluationmentioning

confidence: 99%

Evaluation of organic fractions of municipal solid waste as renewable feedstock for succinic acid production

et al. 2020

Preprint

Background: Despite its high market potential, bio-based succinic acid production experienced recently a declining trend because the initial investments did not meet the expectations for rapid market growth. Thus, reducing the succinic acid production cost is imperative to ensure industrial implementation. Results: Succinic acid production has been evaluated using hydrolysates from the organic fraction of municipal solid waste (OFMSW) collected from MSW treatment plants. A tailor-made enzymatic cocktail was used for OFMSW hydrolysate production containing up to 107.3 g/L carbon sources and up to 638.7 mg/L free amino nitrogen. The bacterial strains Actinobacillus succinogenes and Basfia succiniciproducens were evaluated for succinic acid production with the latter strain being less efficient due to high lactic acid production. Batch A. succinogenes cultures supplemented with 5 g/L yeast extract and 5 g/L MgCO3 reached 29.4 g/L succinic acid with productivity of 0.89 g/L/h and yield of 0.56 g/g. Continuous cultures at dilution rate of 0.06 h-1 reached 21.2 g/L succinic acid with yield of 0.47 g/g and productivity of 1.27 g/L/h. Downstream separation and purification of succinic acid was achieved by centrifugation, treatment with activated carbon, acidification with cation exchange resins, evaporation and drying, reaching more than 99% purity. Preliminary techno-economic evaluation has been employed to evaluate the profitability potential of bio-based succinic acid production.Conclusions: The use of OFMSW hydrolysate in continuous cultures could reduce succinic acid production cost to 2.07 $/kg at annual production capacity of 40,000 t and OFMSW hydrolysate production cost of 25 $/kg sugars.