Optimal immobilization of Trichoderma asperellum laccase on polymer coated Fe3O4@SiO2 nanoparticles for enhanced biohydrogen production from delignified lignocellulosic biomass

Shanmugam, Sabarathinam; Swaminathan, K.; Rejeeth, Chandrababu; Veerabagu, Udayakumar; Pugazhendhi, Arivalagan; Mathimani, Thangavel

doi:10.1016/j.fuel.2020.117777

Cited by 121 publications

(29 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…have also been employed as a microbial cell factory system for the heterologous production of enzymes (8)(9)(10) that are used for biofuel production in industry (11,12), and in numerous other industries, including production of animal feed (13). As the scientific and practical value has continuously been explored, studies involving Trichoderma species have emerged in a variety of research fields and have shown sustained growth.…”

mentioning

confidence: 99%

MIST: a Multilocus Identification System for Trichoderma

Dou

et al. 2020

Appl Environ Microbiol

View full text Add to dashboard Cite

Due to the rapid expansion in microbial taxonomy, precise identification of common industrially and agriculturally relevant fungi such as Trichoderma species is challenging. In this study, we introduce the on-line multiloci identification system (MIST) for automated detection of three hundred and forty-nine Trichoderma species based on a set of three DNA barcodes. MIST is based on the reference databases of validated sequences of three commonly used phylogenetic markers collected from public databases. The databases consist of four hundred and sixteen complete sequences of the nuclear rRNA internal transcribed spacers 1 and 2 (ITS), five hundred and eighty-three sequence fragments of the gene encoding translation elongation factor 1-alpha (tef1) and five hundred and thirty-four sequence fragments of the gene encoding RNA polymerase subunit 2 (rpb2). Through MIST, information from different DNA barcodes can be combined and the identification of Trichoderma species can be achieved based on the integrated parametric sequence similarity search (blastn) performed in the manner of a decision tree classifier. In the verification process, MIST provided correct identification for forty-four Trichoderma spp. based on DNA barcodes consisting from tef1 and rpb2 markers. Thus, MIST can be used to obtain an automated species identification as well as to retrieve sequences required for the manual identification by means of phylogenetic analysis. IMPORTANCE The genus Trichoderma is important to human kind, with a wide range of applications in industry, agriculture and bioremediation. Thus, quick and accurate identification of Trichoderma species is paramount since it is usually the first step in Trichoderma-based research. However, it frequently becomes a limitation, especially for researchers who lack taxonomic knowledge of fungi. Moreover, as the number of Trichoderma-based studies has increased, a growing number of unidentified sequences have been stored in public databases, which has made the species identification more ambiguous. In this study, we provide an easy to use tool for automated species identification (MIST), a list of Trichoderma species, and corresponding sequences of reference DNA barcodes. Therefore, this study will facilitate the research on the biodiversity and applications of the genus Trichoderma.

show abstract

mentioning

confidence: 99%

MIST: a Multilocus Identification System for Trichoderma

Dou

et al. 2020

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…By a non-linear regression model, 129% was recorded to be the highest enhancement at the dosage of 50 mg/L Ni-Gr nanocomposites at an initial pH of 5.0. Recalcitrant lignin in the lignocellulosic biomass is a major barrier in biofuel production as depicted by Shanmugam et al 146 to convert it into biofuels, removal of recalcitrant lignin is important from the lignocellulosic biomass. An approach of enzyme-mediated degradation of lignin without generating any toxic intermediates was used.…”

Section: Biohydrogen Productionmentioning

confidence: 99%

Nanomaterials: stimulants for biofuels and renewables, yield and energy optimization

et al. 2021

View full text Add to dashboard Cite

show abstract

“…A recent study by Shanmugam et al [65] reported enhanced biohydrogen production from pretreated sweet sorghum stover using immobilized laccase for delignification. Enzymes' optimal parameters were determined using an experimental design for the EDCmediated crosslinking of laccase on silica-coated MNPs functionalized with chitosan.…”

Section: Immobilization Of Lignin-degrading Enzymesmentioning

confidence: 99%

Magnetic Nanomaterials as Biocatalyst Carriers for Biomass Processing: Immobilization Strategies, Reusability, and Applications

2021

View full text Add to dashboard Cite

Environmental concerns, along with oil shortages, have increased industrial interest in biomass conversion to produce biofuels and other valuable chemicals. A green option in biomass processing is the use of enzymes, such as cellulases, hemicellulases, and ligninolytic (laccase and peroxidases), which have outstanding specificity toward their substrates and can be reused if immobilized onto magnetic nanocarriers. Numerous studies report the biocatalysts’ performance after covalent binding or adsorption on differently functionalized magnetic nanoparticles (MNPs). Functionalization strategies of MNPs include silica-based surfaces obtained through a sol–gel process, graphene oxide-based nanocomposites, polymer-coated surfaces, grafting polymer brushes, and others, which have been emphasized in this review of the immobilization and co-immobilization of enzymes used for biomass conversion. Careful analysis of the parameters affecting the performance of enzyme immobilization for new hybrid matrices has enabled us to achieve wider tolerance to thermal or chemical stress by these biosystems during saccharification. Additionally, it has enabled the application of immobilized laccase to remove toxic organic compounds from lignin, among other recent advances addressed here related to the use of reusable magnetic carriers for bioderived chemical manufacturing.

show abstract

Optimal immobilization of Trichoderma asperellum laccase on polymer coated Fe3O4@SiO2 nanoparticles for enhanced biohydrogen production from delignified lignocellulosic biomass

Cited by 121 publications

References 49 publications

MIST: a Multilocus Identification System for Trichoderma

MIST: a Multilocus Identification System for Trichoderma

Nanomaterials: stimulants for biofuels and renewables, yield and energy optimization

Magnetic Nanomaterials as Biocatalyst Carriers for Biomass Processing: Immobilization Strategies, Reusability, and Applications

Contact Info

Product

Resources

About