Optimal markers for the identification of Colletotrichum species

Vieira, Willie Anderson dos Santos; Bezerra, Priscila Alves; Silva, Anthony Carlos da; Veloso, Josiene Silva; Câmara, Marcos Paz Saraiva; Doyle, Vinson P.

doi:10.1016/j.ympev.2019.106694

Cited by 87 publications

(60 citation statements)

References 65 publications

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“…Vieira et al . (2019) demonstrated that GAPDH had the most considerable barcode gap distance in 7 of the 11 species complexes evaluated. The percentage overlap between intra‐ and inter‐specific distances was <20%, placing it among the best barcode candidates across Colletotrichum as a whole.…”

Section: Discussionmentioning

confidence: 99%

“…However, to confirm this finding, β‐tubulin2 (TUB2) and HIS3 sequences should be analysed, taking into account that Vieira et al . (2019) found that those sequences are the most powerful genes identified thus far capable of discriminating species within the C. orchidearum complex. Moreover, the isolates within this complex presented considerable variability of morphological and physiological characteristics such as size and shape of conidia, colony colour, mycelium and use of different carbon sources.…”

Section: Discussionmentioning

confidence: 99%

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Conyza bonariensis as an alternative host for Colletotrichum species in Argentina

Bonacci

Formento²,

Morales

et al. 2020

J Appl Microbiol

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Aims This study investigated the diversity of Colletotrichum isolates recovered from Conyza bonariensis leaves through the use of morphological characteristics, growth rate, carbon sources utilization and phylogenetic analysis. Methods and Results In all, 30 Colletotrichum isolates recovered from C. bonariensis leaves showing symptoms of disease were included in the present study. Based on the analysis of morphology and sequences, the isolates were distributed into six Colletotrichum species complexes. The concatenated alignment of GAPDH and ITS sequences showed that 20 out of 30 isolates were included in four species complexes which comprise the most important pathogens causing anthracnose in soybean or anthracnose and stalk rot in maize: C. truncatum, C. orchidearum, C. gloeosporioides and C. graminicola. The remaining 10 isolates were included in the C. boninense and C. destructivum species complexes or could not be assigned to any complex with the available information. Conclusion Weeds belonging to genus Conyza are host to soybean and maize potential pathogenic species of Colletotrichum and could have a role as inoculum reservoir for cross contamination in the agroecosystem. Significance and Impact of the Study The combined use of morphological, kinetics and physiological parameters of growth and phylogenetic analysis in Colletotrichum isolates from Conyza leaves allowed the detection of species complexes previously not identified in Argentina.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Conyza bonariensis as an alternative host for Colletotrichum species in Argentina

Bonacci

Formento²,

Morales

et al. 2020

J Appl Microbiol

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“…Because it might be unrealistic for most researchers to sequence multiple loci across a large set of isolates, it is useful to recommend markers with more phylogenetic informativeness (Vieira et al., 2020). In the majority of the 14 Colletotrichum species complexes GAPDH , HIS3 , and TUB2 were found to be the most variable and informative markers for discriminating species.…”

Section: Identification and Molecular Diagnosticsmentioning

confidence: 99%

“…Considering the importance of the Colletotrichum genus as a plant pathogen worldwide, rapid identification of a large collection of Colletotrichum isolates is often required. However, there is no consensus on the best molecular markers to discriminate species in each Colletotrichum s.c. (Vieira et al, 2020). Currently, there is no minimum or optimal standard set of molecular markers able to discriminate all the Colletotrichum s.c. (Marin-Felix et al, 2017;Vieira et al, 2020).…”

Section: Molecular Identificationmentioning

confidence: 99%

“…However, there is no consensus on the best molecular markers to discriminate species in each Colletotrichum s.c. (Vieira et al, 2020). Currently, there is no minimum or optimal standard set of molecular markers able to discriminate all the Colletotrichum s.c. (Marin-Felix et al, 2017;Vieira et al, 2020). In general, five markers are amongst those commonly used to differentiate species among the distinct Colletotrichum s.c., especially those associated with soybean anthracnose: ITS, GAPDH, TUB2, CHS-1, and ACT (Damm et al, 2009(Damm et al, , 2019.…”

Section: Molecular Identificationmentioning

confidence: 99%

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Soybean anthracnose caused by Colletotrichum species: Current status and future prospects

Boufleur

Ciampi‐Guillardi

Tikami

et al. 2021

Molecular Plant Pathology

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Soybean (Glycine max) is one of the most important cultivated plants worldwide as a source of protein‐rich foods and animal feeds. Anthracnose, caused by different lineages of the hemibiotrophic fungus Colletotrichum, is one of the main limiting factors to soybean production. Losses due to anthracnose have been neglected, but their impact may threaten up to 50% of the grain production. Taxonomy While C. truncatum is considered the main species associated with soybean anthracnose, recently other species have been reported as pathogenic on this host. Until now, it has not been clear whether the association of new Colletotrichum species with the disease is related to emerging species or whether it is due to the undergoing changes in the taxonomy of the genus. Disease symptoms Typical anthracnose symptoms are pre‐ and postemergence damping‐off; dark, depressed, and irregular spots on cotyledons, stems, petioles, and pods; and necrotic laminar veins on leaves that can result in premature defoliation. Symptoms may evolve to pod rot, immature opening of pods, and premature germination of grains. Challenges As accurate species identification of the causal agent is decisive for disease control and prevention, in this work we review the taxonomic designation of Colletotrichum isolated from soybean to understand which lineages are pathogenic on this host. We also present a comprehensive literature review of soybean anthracnose, focusing on distribution, symptomatology, epidemiology, disease management, identification, and diagnosis. We consider the knowledge emerging from population studies and comparative genomics of Colletotrichum spp. associated with soybean providing future perspectives in the identification of molecular factors involved in the pathogenicity process. Useful website Updates on Colletotrichum can be found at http://www.colletotrichum.org/. All available Colletotrichum genomes on GenBank can be viewed at http://www.colletotrichum.org/genomics/.

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A real‐time PCR for detection of pathogens of anthracnose on Chinese fir using TaqMan probe targeting ApMat gene

Sun

et al. 2022

Pest Management Science

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BACKGROUND: Anthracnose is one of the most widespread and destructive diseases on Chinese fir. Colletotrichum cangyuanense, Colletotrichum fructicola, Colletotrichum gloeosporioides, and Colletotrichum siamense are the causal agents of anthracnose on Chinese fir. A rapid and accurate diagnosis of different pathogens is critical for the disease management.RESULTS: Phylogenetic tree and sequence similarity analysis showed that the single-locus ApMat provides superior phylogenetic information and is an appropriate target to distinguish C.

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Optimal markers for the identification of Colletotrichum species

Cited by 87 publications

References 65 publications

Conyza bonariensis as an alternative host for Colletotrichum species in Argentina

Conyza bonariensis as an alternative host for Colletotrichum species in Argentina

Soybean anthracnose caused by Colletotrichum species: Current status and future prospects

A real‐time PCR for detection of pathogens of anthracnose on Chinese fir using TaqMan probe targeting ApMat gene

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