Optimal parameters for laser tissue soldering. Part I: Tensile strength and scanning electron microscopy analysis

McNally, Karen M.; Sorg, Brian S.; Chan, Eric K. H.; Welch, Ashley J.; Dawes, Judith M.; Owen, Earl

doi:10.1002/(sici)1096-9101(1999)24:5<319::aid-lsm2>3.3.co;2-e

Cited by 22 publications

(27 citation statements)

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“…The implant was induced for 60 seconds until a temperature of 80°C was reached. [22][23][24] histological and ultrastructural analysis Following MRI assessment, all animals were sacrificed and histologically analyzed ( removal from the cadavers, tissue samples were fixed overnight in a 5% buffered aqueous solution of formaldehyde (Pharmacy Inselspital, Bern, Switzerland), and routinely processed in paraffin. Consecutive serial sections of 3 µm thickness were alternately stained with hematoxylin and eosin and Prussian Blue.…”

Section: Induction Heatingmentioning

confidence: 99%

Metabolic pathway and distribution of superparamagnetic iron oxide nanoparticles: in vivo study

Schlachter

Widmer²,

Bregy³

et al. 2011

IJN

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Background: Experimental tissue fusion benefits from the selective heating of superparamagnetic iron oxide nanoparticles (SPIONs) under high frequency irradiation. However, the metabolic pathways of SPIONs for tissue fusion remain unknown. Hence, the goal of this in vivo study was to analyze the distribution of SPIONs in different organs by means of magnetic resonance imaging (MRI) and histological analysis after a SPION-containing patch implantation. Methods: SPION-containing patches were implanted in rats. Three animal groups were studied histologically over six months. Degradation assessment of the SPION-albumin patch was performed in vivo using MRI for iron content localization and biodistribution. Results: No SPION degradation or accumulation into the reticuloendothelial system was detected by MRI, MRI relaxometry, or histology, outside the area of the implantation patch. Concentrations from 0.01 µg/mL to 25 µg/mL were found to be hyperintense in T1-like gradient echo sequences. The best differentiation of concentrations was found in T2 relaxometry, susceptibility-sensitive gradient echo sequences, and in high repetition time T2 images. Qualitative and semiquantitative visualization of small concentrations and accumulation of SPIONs by MRI are feasible. In histological liver samples, Kupffer cells were significantly correlated with postimplantation time, but no differences were observed between sham-treated and induction/no induction groups. Transmission electron microscopy showed local uptake of SPIONs in macrophages and cells of the reticuloendothelial system. Apoptosis staining using caspase showed no increased toxicity compared with sham-treated tissue. Implanted SPION patches were relatively inert with slow, progressive local degradation over the six-month period. No distant structural alterations in the studied tissue could be observed. Conclusion: Systemic bioavailability may play a role in specific SPION implant toxicity and therefore the local degradation process is a further aspect to be assessed in future studies.

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Section: Induction Heatingmentioning

confidence: 99%

Metabolic pathway and distribution of superparamagnetic iron oxide nanoparticles: in vivo study

Schlachter

Widmer²,

Bregy³

et al. 2011

IJN

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“…The new membranes offer flexibility along with improved repair strength over previous published results using albumin protein solders. [14][15][16] The properties of the solder-doped membranes can be easily tailored to meet the specific requirements of a wide range of clinical applications. Both the albumin protein solder and the polymer membranes are biodegradable, and thus, foreign body reaction and infection are expected to be minimal.…”

Section: Discussionmentioning

confidence: 99%

“…7 Current laser repair technology makes use of chromophore-enhanced protein solders. [8][9][10][11][12][13][14][15][16] Laser tissue soldering is a bonding technique in which protein solder is applied to the tissue surfaces to be joined, and laser energy is used to bond the solder to the tissue surfaces. The addition of protein solders to augment tissue repair procedures significantly reduces the problems of low strength and thermal damage associated with laser tissue welding techniques.…”

Section: Introductionmentioning

confidence: 99%

Improved vascular tissue fusion using new light-activated surgical adhesive on a canine model

McNally

Sorg

Hammer³

et al. 2001

J. Biomed. Opt.

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Newly developed light-activated surgical adhesives have been investigated as a substitute to traditional protein solders for vascular tissue fusion without the need for sutures. Canine femoral arteries (n = 14), femoral veins (n = 14), and carotid arteries (n = 10) were exposed, and a 0.3-0.6 cm longitudinal incision was made in the vessel walls. The surgical adhesive, composed of a poly(L-lactic-co-glycolic acid) scaffold doped with the traditional protein solder mix of bovine serum albumin and indocyanine green dye, was used to close the incisions in conjunction with an 805 nm diode laser. Blood flow was restored to the vessels immediately after the procedure and the incision sites were checked for patency. The new adhesives were flexible enough to be wrapped around the vessels while their solid nature avoided the problems associated with "runaway" of the less viscous liquid protein solders widely used by researchers. Assessment parameters included measurement of the ex vivo intraluminal bursting pressure 1-2 h after surgery, as well as histology. The acute intraluminal bursting pressures were significantly higher in the laser-solder group (>300 mmHg) compared to the suture control group (<150 mmHg) where four evenly spaced sutures were used to repair the vessel (n = 4). Histological analysis showed negligible evidence of collateral thermal damage to the underlying tissue in the laser-solder repair group. These initial results indicated that laser-assisted vascular repair using the new adhesives is safe, easy to perform, and contrary to conventional suturing, provides an immediate leak-free closure. In addition, the flexible and moldable nature of the new adhesives should allow them to be tailored to a wide range of tissue geometries, thus greatly improving the clinical applicability of laser-assisted tissue repair.

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“…The technique is based on the sutureless coaptation of vessel segments by photothermal or photochemical [48] processes. Photothermal laser vessel bonding currently comprises the most frequently employed technique and can be divided into two distinct modalities, namely native laser-assisted vascular welding (LAVW) and modalities where proteinaceous solders are used to adjoin vessel segments, namely solder-enhanced LAVW (sLAVW) [49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64][65][66][67].…”

Section: Introductionmentioning

confidence: 99%

Laser-assisted vessel welding: state of the art and future outlook

Pabittei

Boon

Heger

et al. 2015

JCTRes

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Laser-assisted vascular welding (LAVW) is an experimental technique being developed as an alternative to suture anastomosis. In comparison to mechanical anastomosis, LAVW is less traumatic, non-immunogenic, provides immediate water tight sealant, and possibly a faster and easier procedure for minimally invasive surgery. This review focuses on technical advances to improve welding strength and to reduce thermal damage in LAVW. In terms of welding strength, LAVW has evolved from the photothermally-induced microvascular anastomosis, requiring stay sutures to support welding strength, to sutureless anastomoses of medium-sized vessels, withstanding physiological and supraphysiological pressure. Further improvements in anastomotic strength could be achieved by the use of chromophore-containing albumin solder and the employment of (biocompatible) polymeric scaffolds. The anastomotic strength and the stability of welds achieved with such a modality, referred to as scaffold-and solder-enhanced LAVW (ssLAVW), are dependent on the intermolecular bonding of solder molecules (cohesive bonding) and the bonding between solder and tissue collagen (adhesive bonding). Presently, the challenges of ssLAVW include (1) obtaining an optimal balance between cohesive and adhesive bonding and (2) minimizing thermal damage. The modulation of thermodynamics during welding, the application of semi-solid solder, and the use of a scaffold that supports both cohesive and adhesive strength are essential to improve welding strength and to limit thermal damage.

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Optimal parameters for laser tissue soldering. Part I: Tensile strength and scanning electron microscopy analysis

Cited by 22 publications

References 0 publications

Metabolic pathway and distribution of superparamagnetic iron oxide nanoparticles: in vivo study

Metabolic pathway and distribution of superparamagnetic iron oxide nanoparticles: in vivo study

Improved vascular tissue fusion using new light-activated surgical adhesive on a canine model

Laser-assisted vessel welding: state of the art and future outlook

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