This work proposes novel techniques toward the design of optimal pilot sequences to perform channel estimation in block transmission systems over wideband frequency selective wireless fading channels. The framework developed is based on minimization of the Bayesian Cramér-Rao bound (BCRB) for the mean squared error (MSE) of the channel state information (CSI) estimate. Optimal pilot signals are determined for the four predominant classes of block transmission systems, viz. single carrier zero padding (SC-ZP), multi-carrier zero padding (MC-ZP), single carrier cyclic prefix (SC-CP) and multicarrier cyclic prefix (MC-CP) systems. This makes the techniques developed general in nature and thus applicable in a wide variety of block transmission systems. As part of this study, succinct expressions and results are also derived to characterize the error rate performance, incorporating also the effect of CSI estimation error resulting due to the proposed algorithms. Finally, numerical results obtained via Monte-Carlo simulation are presented to illustrate and compare the CSI acquisition performance of optimal pilot designs with that of conventional designs and also validate the theoretical analysis for the error rate performance. INDEX TERMS Block transmission system, mean square error (MSE), bit error rate (BER), diversity, minimum mean square error (MMSE), maximum likelihood (ML), zero forcing (ZF)