Optimal Point Spread Function Design for 3D Imaging

Shechtman, Yoav; Sahl, Steffen J.; Backer, Adam S.; Moerner, W. E.

doi:10.1103/physrevlett.113.133902

Cited by 336 publications

(326 citation statements)

References 32 publications

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“…This framework extends previous work applying Fisher information to individual imaging techniques (e.g., Helstrom, 1969;Winick, 1986;Ober et al, 2004;Aguet et al, 2005;Shahram, 2005;Shahram and Milanfar, 2006;Marengo et al, 2009;Sanches et al, 2010;Mukamel and Schnitzer, 2012;Quirin et al, 2012;Shechtman et al, 2014). For example, many studies have used Fisher information to examine the theoretical optimal resolution of specific optical imaging techniques (Helstrom, 1969;Winick, 1986;Ober et al, 2004;Aguet et al, 2005;Shahram, 2005;Shahram and Milanfar, 2006;Marengo et al, 2009;Mukamel and Schnitzer, 2012;Quirin et al, 2012;Shechtman et al, 2014). However, using Fisher information to optimize other neural recording technologies, while occasionally done (e.g., MRI in Sanches et al, 2010), is not as common.…”

Section: Framework Discussionmentioning

confidence: 64%

Spatial Information in Large-Scale Neural Recordings

Cybulski¹,

Glaser²,

Marblestone

et al. 2014

Preprint

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To record from a given neuron, a recording technology must be able to separate the activity of that neuron from the activity of its neighbors. Here, we develop a Fisher information based framework to determine the conditions under which this is feasible for a given technology. This framework combines measurable point spread functions with measurable noise distributions to produce theoretical bounds on the precision with which a recording technology can localize neural activities. If there is sufficient information to uniquely localize neural activities, then a technology will, from an information theoretic perspective, be able to record from these neurons. We (1) describe this framework, and (2) demonstrate its application in model experiments. This method generalizes to many recording devices that resolve objects in space and should be useful in the design of next-generation scalable neural recording systems.

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Section: Framework Discussionmentioning

confidence: 64%

Spatial Information in Large-Scale Neural Recordings

Cybulski¹,

Glaser²,

Marblestone

et al. 2014

Preprint

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“…This motion blur obscures the image and contributes additional localization uncertainty. In addition, because of the axial symmetry of the point spread function (PSF) these methods cannot natively track particles in three dimensions without augmenting the instrument with specialized optics that distort the image with a deterministic axial dependence [8][9][10][11] or with multiple cameras to image multiple planes simultaneously [12]. Many of these methods have limited axial range or limit the measured intensity on any single detector by spreading the photons out over multiple detectors.…”

Section: Introductionmentioning

confidence: 99%

Tracking single fluorescent particles in three dimensions via extremum seeking

Ashley¹,

Gan²,

Pan³

et al. 2016

Biomed. Opt. Express

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The ability to track single fluorescent particles in three-dimensions with subdiffraction limit precision as well as sub-millisecond temporal resolution has enabled the understanding of many biophysical phenomena at the nanometer scale. While there are several techniques for achieving this, most require complicated experimental setups that are expensive to implement. These methods can offer superb performance but their complexity may be overwhelming to the end-user whose aim is only to understand the feature being imaged. In this work, we describe a method for tracking a single fluorescent particle using a standard confocal or multi-photon microscope configuration. It relies only on the assumption that the relative position of the measurement point and the particle can be actuated and that the point spread function has a global maximum that coincides with the particle's position. The method uses intensity feedback to calculate real-time position commands that "seek" the extremum of the point spread function as the particle moves through its environment. We demonstrate the method by tracking a diffusing quantum dot in a hydrogel on a standard epifluorescent confocal microscope.

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“…The lower half of Figure 22 shows the application of the DH-PSF method to 3D surface imaging of Caulobacter labeled by the rhodamine spirolactam (172). Much remains to be done, as new point-spread function designs continue to appear (180)(181)(182), with the continuing goal to extract the maximum information from each tiny single-molecule emitter in the most efficient fashion.…”

Section: Figure 22 Here -mentioning

confidence: 99%

Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy

Moerner

2015

Rev. Mod. Phys.

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The initial steps toward optical detection and spectroscopy of single molecules in condensed matter arose out of the study of inhomogeneously broadened optical absorption profiles of molecular impurities in solids at low temperatures. Spectral signatures relating to the fluctuations of the number of molecules in resonance led to the attainment of the single-molecule limit in 1989 using frequency-modulation laser spectroscopy. In the early 90's, many fascinating physical effects were observed for individual molecules, and the imaging of single molecules as well as observations of spectral diffusion, optical switching and the ability to select different single molecules in the same focal volume simply by tuning the pumping laser frequency provided important forerunners of the later superresolution microscopy with single molecules. In the room temperature regime, imaging of single copies of the green fluorescent protein also uncovered surprises, especially the blinking and photoinduced recovery of emitters, which stimulated further development of photoswitchable fluorescent protein labels. Because each single fluorophore acts a light source roughly 1 nm in size, microscopic observation and localization of individual fluorophores is a key ingredient to imaging beyond the optical diffraction limit. Combining this with active control of the number of emitting molecules in the pumped volume led to the super-resolution imaging of Eric Betzig and others, a new frontier for optical microscopy beyond the diffraction limit. The background leading up to these observations is described and current developments are summarized. The early days Introduction and early inspirationsI want to thank the Nobel Committee for Chemistry, the Royal Swedish Academy of Sciences, and the Nobel Foundation for selecting me for this prize recognizing the development of super-resolved fluorescence microscopy. I am truly honored to share the prize with my two esteemed colleagues, Stefan Hell and Eric Betzig. My primary contributions center on the first optical detection and spectroscopy of single molecules in the condensed phase(1), and on the observations of imaging, blinking and photocontrol not only for single molecules at low temperatures in solids, but also for useful variants of the green fluorescent protein at room temperature (2). This lecture describes the context of the events leading up to these advances as well as a portion of the subsequent developments both around the world and in my laboratory.In the mid-1980's, I derived much early inspiration from amazing advances that were occurring around the world where single nanoscale quantum systems were detected and explored for both scientific and technological reasons. Some of these were (i) the spectroscopy of single electrons or ions confined in vacuum electromagnetic traps (3-5), (ii) scanning tunneling microscopy (STM) (6) and atomic force microscopy (AFM) (7), and (iii) the study of ion currents in single membrane-embedded ion channels (8). But why not optical detection and ...

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Optimal Point Spread Function Design for 3D Imaging

Cited by 336 publications

References 32 publications

Spatial Information in Large-Scale Neural Recordings

Spatial Information in Large-Scale Neural Recordings

Tracking single fluorescent particles in three dimensions via extremum seeking

Nobel Lecture: Single-molecule spectroscopy, imaging, and photocontrol: Foundations for super-resolution microscopy

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