Optimal use of fresh and frozen-thawed testicular sperm for intracytoplasmic sperm injection in azoospermic patients

Wu, Bin; Wong, Daniel; Lu, Suzhen; Dickstein, Stephen; Silva, Marta; Gelety, Timothy J.

doi:10.1007/s10815-005-7481-y

Cited by 53 publications

(54 citation statements)

References 23 publications

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“…The fertilization rates achieved with fresh vs. frozen-thawed testicular spermatozoa were 69% vs. 61.7%, the pregnancy rates per embryo transfer were 29.1% vs. 25.0%, and the delivery rates were 85.7% vs. 80.0%, respectively [Rhouma et al 2003]. Wu et al [2005] achieved a fertilization rate of 72% using fresh testicular spermatozoa and 67% using frozen-thawed testicular spermatozoa. A higher proportion of blastocysts have been shown to develop when frozen-thawed spermatozoa are used, compared with fresh spermatozoa [Zorn et al 2009].…”

Section: Discussionmentioning

confidence: 99%

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

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This study was performed to assess and compare the outcomes of testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI) using spermatozoa from fresh and frozen testicular tissue from men with subgroups of non-obstructive azoospermia (NOA). A total of 110 cycles of TESE-ICSI were performed. Patients were classified into one of the following NOA subgroups: hypospermatogenesis (HS), maturation arrest (MA), or Sertoli cell-only syndrome (SCO). Laboratory (fertilization, cleavage stage of embryo, and good quality embryo) and clinical (pregnancy, clinical pregnancy, implantation, and delivery) outcomes were assessed. No statistically significant differences were observed in any of the other measured parameters between the three subgroups of NOA. No significant differences in laboratory outcomes were observed between spermatozoa from fresh and frozen testicular spermatozoa; however, statistically significant differences were observed in the pregnancy and implantation rates between groups (p50.05). The outcomes of using spermatozoa retrieved from fresh testicular tissue in each of the three subgroups were also compared; although clinical outcomes showed low results, no significant differences were observed between the three subgroups. Similarly, no significant differences were observed in spermatozoa retrieved from frozen testicular tissue. Once spermatozoa have been successfully obtained, acceptable laboratory outcomes can be achieved for NOA, whether or not the spermatozoa are cryopreserved. However, satisfactory clinical outcomes may be more difficult to achieve as the results showed in each group of fresh and frozen testicular spermatozoa. Therefore, achieving acceptable clinical pregnancy results and efficient cryopreservation of testicular spermatozoa should be considered in patients with NOA.

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Section: Discussionmentioning

confidence: 99%

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

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“…testicular spermatozoa or tissue are reliable approaches for the management of azoospermic patients and could all for the possibility of multiple IVF-ICSI procedures [7]. Previously Park et al [8] reported that acceptable fertilization and pregnancy rates were achieved using frozen testicular spermatozoa as opposed to fresh testicular spermatozoa in obstructive azoospermia (OA).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Park

Kim

Lim

et al. 2014

J Assist Reprod Genet

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Purpose To evaluate the viability of frozen embryos generated by intracytoplasmic sperm injection (ICSI) with frozen testicular spermatozoa. Methods A total of 68 fresh embryo transfer (ET) cycles and 85 subsequent frozen-thawed ET (FET) cycles were grouped according to the source of spermatozoa: fresh testicular spermatozoa (TESE) or frozen-thawed testicular spermatozoa (t-TESE). Results There were no significant differences in the age of female patients, number of oocytes, or fertilization rates in fresh ET cycles with TESE (TESE-fresh ET) versus t-TESE (t-TESE-fresh ET). The rate of embryo survival after thawing (95.7 % vs. 94.0 %) was similar in frozen ET cycles (FET) with TESE (TESE-FET) and with t-TESE (t-TESE-FET). While there were significant differences in the proportion of good quality embryos, no statistical differences were found in the pregnancy or clinical abortion rates between the two groups. Moreover, delivery rates were not significantly different. Conclusions Although the proportion of good quality embryos was affected by cryopreservation of testicular tissue, embryo survival rate was not. As well, subsequent pregnancy could be achieved successfully via t-TESE-FET cycles. Therefore, FET is not affected by the cryopreservation of testicular tissue, and avoids further oocyte retrieval and TESE procedures.

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“…Although testicular sperm extraction is used frequently to obtain sufficient sperm for ICSI, very few spermatozoa demonstrate twitching motility or are completely immotile in the initial testicular biopsy samples. Our research observed less than 3% motile sperm (slight twitching) during the initial collection of testicular biopsy samples (Wu et al, 2005). In most cases, it is very difficult to find sufficient motile sperm in the initial or frozen-thawed TESE samples.…”

Section: In Vitro Maturation Of Testicular Biopsy Tissuementioning

confidence: 52%

“…Our further experiment showed that after 24 hour in vitro culture, the number of motile sperm increases remarkably, with a maximum motility rate seen between 48-72 hours of culture (Figure 2). Motile spermatozoa still are observed up to 120 hours in culture (Wu et al, 2005). Based on our research and clinical practice, it appears that the optimal time for ICSI using testicular sperm is after 24-48 hours of culture.…”

Section: In Vitro Maturation Of Testicular Biopsy Tissuementioning

confidence: 76%

“…In the last decade, many groups have suggested methods to optimize the use of testicular biopsy specimens. Several strategies have been developed to optimally manage a testicular biopsy, including improvement of sperm motility and increasing the motile sperm recovery rate after freezing (Wu et al, 2005). In most cases, only few spermatozoa can be obtained from minced testicular tissue and many of these free spermatozoa display complete immotility and occasionally only an individual sperm shows a slowly "twitching" movement.…”

Section: Optimal Use Of Testicular Biopsy Spermmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Fertility Options of Azoospermic Men

Wu¹,

Gelety²,

Shi³

2012

Advances in Embryo Transfer

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Optimal use of fresh and frozen-thawed testicular sperm for intracytoplasmic sperm injection in azoospermic patients

Abstract: The freezing and in vitro culturing of testicular biopsy tissue is a very reliable approach for the management of testicular biopsy specimens from azoospermic patients, and offers the possibility of several treatments of IVF/ICSI from a single sample.

Cited by 53 publications

References 23 publications

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Efficacy of cryopreservation of embryos generated by intracytoplasmic sperm injection with spermatozoa from frozen testicular tissue

Advances in Fertility Options of Azoospermic Men

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