Optimation of Combination of N-Hexane Solution and Ethyle Acetate on Secondary Metabolite Compounds Profile of Streptomyces hygroscopicus

Abstract

<p>Streptomyces hygroscopicus (S.hygroscopicus) is a Gram-positive soil bacterium that can produce secondary metabolites from fermentation that have a therapeutic effect. The fermented S. hygrocospicus metabolites that are still in the form of crude extracts are difficult to develop as drug preparations because the active compounds are not yet known, so it will be challenging to determine the dosage of drugs that have a therapeutic effect. Therefore, it is necessary to carry out exploratory research to narrow down the secondary metabolite profile from the fermentation of S. hygroscopicus, using extraction and fractionation methods, which are then identified by Thin-Layer Chromatography (TLC) using a combination of solvents. This study used the extraction method with a separating funnel. The fractionation was carried out using the BUCHI (Sepacore®) Flash Chromatography and Reveleris® PREP Purification System column chromatography gradually using ethyl acetate and n-hexana. 47 and 60 of the fractionation results were taken as samples, that further were profiled using TLC and given the appearance of 10% KOH stains and p-Anisaldehyde - sulfuric acid, so that various classes of compounds with different Rf values were obtained, namely Monoterpenes, Triterpenes, Steroids, Saponins, Coumarin, Scopoletin, and Alkaloids.</p>

Fractions 14 and 36K of Metabolite Extract Streptomyces hygroscopicus subsp. Hygroscopicus Have Antimalarial Activities Against Plasmodium berghei in vitro

“…Previous TLC results found alkaloid compounds in the fraction 36K, 17 this is in accordance with the discovery of tryptanthrin, a weak alkaloid compound in another fraction of S. hygroscopicus subsp. Hygroscopicus extract.…”

“…According to the fractionation carried out in an earlier study, 17 , 18 there were a total of 47 fractions from fractionation I (Sepacore ® Flash Chromatography) and 60 fractions from fractionation II (Reveleris ® PREP Purification System Chromatography), and in each tube, a different weight of fraction was produced during fractionation. The fractionation was done using column chromatography with hexane and ethyl acetate solvents to simplify the extract samples containing various components.…”

“…In our previous study, 17 fractionation of metabolite extracts of S. hygroscopicus subsp. Hygroscopicus was conducted using the BUCHI Reveleris ® PREP Purification System flash column with silica gel 60 eluted by n-hexane and ethyl acetate in a ratio of 1:1 (v/v).…”

“…In previous research, we have shown that both fractions contain monoterpene, triterpene, and steroid chemicals (fraction I), as well as coumarins, scopoletin, and alkaloids (fraction II), which may have antimalarial activity. 17 Monoterpene and triterpene compounds are included in the class of terpenoid compounds. Terpenoid compounds and their derivatives have a structure that allows them to cross the lipid bilayer of the erythrocyte membrane and prevent parasite cells from the synthesis of proteins.…”

Uji Efektivitas Ekstrak Metanol Buah Pare (Momordica Charantia L.) Terhadap Pertumbuhan Aspergillus Niger

“…Pelarut etil asetat diketahui memiliki sifat semi polar, sedangkan pelarut heksan diketahui bersifat non-polar. (Ariel et al, 2021) Pelarut lain yang diketahui memiliki sifat non-polar yaitu aseton, petroleum, eter, etanol, dan benzene. Senyawa-senyawa fitokimia yang diketahui memiliki sifat polar diantaranya yaitu fenolik, asam amino, terpenoid, glikosida, flavonoid, antosianin, saponin, tannin, xantosilin, totarol, quasinoid, lakton, fenon, flavon, dan polifenol.…”