Optimisation and validation of ultra-high performance liquid chromatographic-tandem mass spectrometry method for qualitative and quantitative analysis of potato steroidal alkaloids

Hossain, Mohammad B.; Dilip, K.; Brunton, Nigel P.

doi:10.1016/j.jchromb.2015.05.033

Cited by 23 publications

(13 citation statements)

References 17 publications

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“…A pre-existing database of MRM transitions of about 55 polyphenols, which included the most commonly found phenolics in barley, was used for screening the phenolic compounds in the bioactive fractions. The ionization source conditions were as described by Hossain, Rai, and Brunton (2015): capillary voltage 3 kV, cone voltage 40 V, extractor voltage 3 V, source temperature 120 ᴼC, desolvation temperature 350 ᴼC, desolvation gas flow 800 L/h, cone gas flow 50 L/h, and collision gas flow 0.1 mL/min. The desolvation gas used was nitrogen.…”

Section: Mass Spectrometric Determination Of Polyphenols In the Antiomentioning

confidence: 99%

Antioxidant-guided isolation and mass spectrometric identification of the major polyphenols in barley (Hordeum vulgare) grain

et al. 2016

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In the present study, the relative contribution of individual/classes of polyphenols in barley, to its antioxidant properties, was evaluated. Flash chromatography was used to fractionate the total polyphenol extract of Irish barley cultivar 'Irina', and fractions with highest antioxidant properties were identified using total phenolic content and three in vitro antioxidant assays: DPPH, FRAP, and ORAC. Flavanols (catechin, procyanidin B, prodelphinidin B, procyanidin C) and a novel substituted flavanol (catechin dihexoside, C27H33O16(-), m/z 613.17), were identified as constituents of the fraction with highest antioxidant capacity. Upon identification of phenolics in the other active fractions, the order of most potent contributors to observed antioxidant capacity of barley extract were, flavanols>flavonols (quercetin)>hydroxycinnamic acids (ferulic, caffeic, coumaric acids). The most abundant polyphenol in the overall extract was ferulic acid (277.7μg/gdw barley), followed by procyanidin B (73.7μg/gdw barley).

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Section: Mass Spectrometric Determination Of Polyphenols In the Antiomentioning

confidence: 99%

Antioxidant-guided isolation and mass spectrometric identification of the major polyphenols in barley (Hordeum vulgare) grain

et al. 2016

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“…Steroidal alkaloids were analysed using Waters Acquity (Waters Corporation, Milford, MA, USA) ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) using a validated method for potato steroidal alkaloids [24]. The recovery rate of the method ranged from 97% to 103% for four steroidal alkaloids analysed.…”

Section: Methodsmentioning

confidence: 99%

Effect of Drying Methods on the Steroidal Alkaloid Content of Potato Peels, Shoots and Berries

2016

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The present study has found that dried potato samples yielded significantly higher levels of steroidal alkaloids such as α-solanine and α-chaconine than the corresponding fresh samples, as determined by the UPLC-MS/MS technique. Among the drying techniques used, air drying had the highest effect on steroidal alkaloid contents, followed by freeze drying and vacuum oven drying. There was no significant difference between the freeze dried and vacuum oven dried samples in their α-chaconine contents. However, freeze dried potato shoots and berries had significantly higher α-solanine contents (825 µg/g dry weight (DW) in shoots and 2453 µg/g DW in berries) than the vacuum oven dried ones (325 µg/g dry weight (DW) in shoots and 2080 µg/g DW in berries). The kinetics of steroidal alkaloid contents of potato shoots during air drying were monitored over a period of 21 days. Both α-solanine and α-chaconine content increased to their maximum values, 875 µg/g DW and 3385 µg/g DW, respectively, after 7 days of drying. The steroidal alkaloid contents of the shoots decreased significantly at day 9, and then remained unchanged until day 21. In line with the potato shoots, air dried potato tuber peels also had higher steroidal alkaloid content than the freeze dried and vacuum oven dried samples. However, a significant decrease of steroidal alkaloid content was observed in air dried potato berries, possibly due to degradation during slicing of the whole berries prior to air drying. Remarkable variation in steroidal alkaloid contents among different tissue types of potato plants was observed with the potato flowers having the highest content.

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“…The two toxins appear to inhibit the human enzyme acetyl cholinesterase [78], the calcium transport [79], the transport through cellular membranes [80,81] and even at low concentrations (micro molar) the compounds may be able to harm nerve cells [82]. In vitro experiments revealed that α-solanine and particularly α-chaconine are effective cytotoxins (IC50 = 4.1 μM) by rapidly inducing cell lysis [83,84]. Toxins accumulation in potatoes occurs mainly in the peel as it represents the first barrier against pathogenic attacks [76].…”

Section: Alkaloids Content In Potatoesmentioning

confidence: 99%

Processing, Valorization and Application of Bio-Waste Derived Compounds from Potato, Tomato, Olive and Cereals: A Review

Fritsch

Stäbler

Happel³

et al. 2017

Sustainability

157

102

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Abstract:The vast and ever-growing amount of agricultural and food wastes has become a major concern throughout the whole world. Therefore, strategies for their processing and value-added reuse are needed to enable a sustainable utilization of feedstocks and reduce the environmental burden. By-products of potato, tomato, cereals and olive arise in significant amounts in European countries and are consequently of high relevance. Due to their composition with various beneficial ingredients, the waste products can be valorized by different techniques leading to economic and environmental advantages. This paper focuses on the waste generation during industrial processing of potato, tomato, cereals and olives within the European Union and reviews state-of-the-art technologies for their valorization. Furthermore, current applications, future perspectives and challenges are discussed.

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Optimisation and validation of ultra-high performance liquid chromatographic-tandem mass spectrometry method for qualitative and quantitative analysis of potato steroidal alkaloids

Cited by 23 publications

References 17 publications

Antioxidant-guided isolation and mass spectrometric identification of the major polyphenols in barley (Hordeum vulgare) grain

Antioxidant-guided isolation and mass spectrometric identification of the major polyphenols in barley (Hordeum vulgare) grain

Effect of Drying Methods on the Steroidal Alkaloid Content of Potato Peels, Shoots and Berries

Processing, Valorization and Application of Bio-Waste Derived Compounds from Potato, Tomato, Olive and Cereals: A Review

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