This study presents a comparative analysis of biogas yields from various organic waste sources in Nakisunga Parish, Mukono District, Uganda, a rural area with significant potential for biogas production. The primary objective was to identify the most efficient waste sources for biogas production, thereby offering insights into optimizing biogas systems in similar rural communities. The study utilized a combination of field surveys and laboratory analyses to evaluate the biogas production potential of farm waste, household waste, municipal waste, and food processing waste. Results indicated that farm waste, particularly swine manure, exhibited the highest biochemical methane potential (BMP), with a BMP value of 0.007 mL CH₄/g VS, making it the most promising feedstock for biogas production. Crop residues and cow dung also showed moderate potential, while household and food processing wastes had lower BMP values. The findings underscore the importance of selecting appropriate feedstocks to maximize biogas yields and highlight the viability of biogas production in rural Ugandan communities where agricultural activities dominate. This study provides localized data on biogas potential in rural Sub-Saharan Africa, addressing the gap in research regarding the comparative efficiency of various organic waste sources in these settings. The results have significant implications for sustainable energy policy and rural development, suggesting that targeted biogas initiatives could play a critical role in improving energy access and waste management in rural areas. Recommendations for future research include exploring co-digestion strategies to further enhance biogas production and examining the socio-economic impacts of biogas adoption in rural communities.