Optimisation of bioflocculant production by a biofilm forming microorganism from poultry slaughterhouse wastewater for use in poultry wastewater treatment

Abstract

Poultry slaughterhouse wastewater contains nutrients that are sufficient for microbial growth; moreover, the wastewater has microorganisms which can be harnessed to perform specific functions. Additionally, these microorganisms can grow either in planktonic (free floating) mode or sessile (attached) mode. This study focused on the optimisation of bioflocculant production by quantifying flocculation activity, determined using kaolin clay (4 g/L), by isolates prevalent in poultry slaughterhouse wastewater. Subsequent to their identification and characterisation, six bacterial strains were initially isolated from the poultry wastewater. Although all the isolated microorganisms produced bioflocculants under different conditions, i.e. pH and temperature, the strain that produced bioflocculants with a higher flocculation activity was isolate BF-3, a Comamonas sp., achieving a flocculation activity of 93.8% at 32.9 °C and pH 6.5. Fourier transform infrared spectroscopy (FTIR) analysis of the bioflocculant of the isolate, showed the presence of hydroxyl, carboxyl, alkane and amine functional groups, an indication that the bioflocculant was a protein constituent.

Use of Leuconostoc Mesenteroides to Produce a Dextran Bioflocculant

“…The influence of pH in different bacteria was also studied by other authors such as Zhao et al (2013) where a high flocculation activity of MBF-5 by Klebsiella pneumoniae in acidic pH (less than 5) was reported. In contrast, Wang et al (2015) evaluated the flocculation activity and flocculation mechanism of bioflocculant (XMMBF) produced by Bacillus licheniformis, obtaining more than 92% of activity at a pH range of 5-12, with a maximum activity of 97% at a pH of 8, demonstrating that pH is a key factor influencing flocculation activity in different reaction systems (Dlangamandla et al, 2016). Because there was no significant difference between the percentage values of reduced turbidity for pH 9, 10 and 11, pH 9 was used.…”

“…The absorbance spectra were recorded at a speed of 16 scans and a resolution of 1 cm −1 . The polymer was dried before use in an oven (Mememrt) at 105°C for 1 h to avoid interference from moisture (Dlangamandla et al, 2016). Then, microscopy and energy dispersive X-ray spectroscopy (EDS) analysis were performed on the bioflocculant produced by L. mesenteroides var.…”

Poultry Slaughterhouse Wastewater Remediation Using a Bio-Delipidation Pre-Treatment Unit Coupled with an Expanded Granular Sludge Bed Reactor

“…Up to 80, 81 and 85% removal for BOD, TSS and COD, respectively. [17,18] Previous studies have focused on identifying a bacterial culture from PSW which has the natural ability to dissolve FOG and tCOD removal [19,20]. There is little focus on currently commercially available products (many of which are readily available, albeit with different qualities) with these capabilities, which would invariantly reduce the need to find suitable organisms, optimize culture conditions to obtain the desired traits of the final product and develop new production systems to manufacture the desired product with an appropriate quality.…”

Assessment of Bacterial Diversity of Industrial Poultry Wastewater by Denaturing Gradient Gel Electrophoresis (DGGE) and the Cultivation Method in Order to Inform Its Reuse in Agriculture

“…It has been estimated that the water consumption average is around 26.5 L per bird, which remains dependent on the degree of automation [ 7 , 8 ]. As a consequence, large quantities of highly polluted wastewater are generated [ 2 , 9 ]. These effluents were classified among the most polluted discharges, due to the high concentration of physico-chemical properties including chemical oxygen demand (COD), biochemical oxygen demand (BOD), and total suspended solids (TSS), as well as nutritive elements (nitrogen and phosphorus) and organic matter including proteins from blood residues and fats from slaughtering and cleaning activities [ 9 – 13 ].…”