Optimisation of carbohydrate, lipid and biomass productivity in<i>Tetradesmus obliquus</i>using response surface methodology

Nadzir, Syafiqah Md; Yusof, Norjan; Nordin, Norazela; Abdullah, Hanisom; Kamari, Azlan

doi:10.1080/17597269.2018.1542568

Cited by 8 publications

(3 citation statements)

References 36 publications

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“…51 Hence, the species responded with various changes in their biochemical pathways, thus the reported ranges of the species' biomass biochemical compositions. Comparing our results to Nadzir et al 53 based on the same growth media with similar constituent composition, we recorded a lower biomass carbohydrate but higher protein and total lipid content. This was expected because Nadzir and colleagues cultivated their strain for only 14 days under different experimental settings (4000 lx illuminance, 12 :12 h, light:dark photoperiod, 26 ± 3 °C temperature and a controlled pH at 7.5) to those used herein (1500-1700 lx, 14 h:10 h photoperiod, 23 ± 2 °C and unregulated pH after an initial value of 6.31).…”

Section: Results and Discussion Sample Collection Isolation And Identificationsupporting

confidence: 76%

Bioresource potential of Tetradesmus obliquusUJEA_AD: critical evaluation of biosequestration rate, biochemical and fatty acid composition in BG11 media

Ahiahonu

Anku

Roopnarain

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: Microalgae biomass is regarded as one of the most promising renewable biomass energy resources with the potential as a sustainable biological climate change mitigation agent, raw material for commercially valuable products and fossil fuel replacement. In view of that, this study isolated, identified and evaluated the potential of an indigenous freshwater green microalga strain, Tetradesmus obliquus UJEA_AD, collected from Emmarentia Dam, Johannesburg, South Africa.RESULTS: Morphological analysis and sequencing of the rbcL biomarker aided in identification of the isolate as T. obliquus. Tetradesmus obliquus UJEA_AD was further batch-cultivated autotrophically in BG11 medium and examined for biomass accumulation, CO 2 biofixation rate, biochemical composition and fatty acid production. The results showed a maximum biomass accumulation of 2.78 ± 0.019 g L −1 with biomass productivity of 0.153 ± 0.001 g L −1 day −1 and specific growth rate of 0.13 ± 0.004 day -1 as well as CO 2 biofixation rate of 0.265 ± 0.002 gCO 2 L −1 day −1 . The biochemical data of the isolate revealed 27.17% ± 0.021% carbohydrate, 38.00% ± 0.15% protein and 31.2% ± 0.80% lipids; with primary fatty acids such as palmitic acid (34.26% ± 2.02%), linolenic acid (18.08 ± 5.29), trans-13-octadecenoic acid (12.32 ± 0.92) and phytol, 2-methylbutanoate (12.31 ± 1.02).CONCLUSION: These findings suggest that T. obliquus UJEA_AD is capable of producing bioproducts of economic value with an excellent CO 2 bio-mitigation potential when cultivated at optimum conditions in an autotrophic mode. Moreover, lipid extracted from T. obliquus UJEA_AD met the biodiesel quality standards of the USA and EU, making the isolate a promising commercial biodiesel feedstock.

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Section: Results and Discussion Sample Collection Isolation And Identificationsupporting

confidence: 76%

Bioresource potential of Tetradesmus obliquusUJEA_AD: critical evaluation of biosequestration rate, biochemical and fatty acid composition in BG11 media

Ahiahonu

Anku

Roopnarain

et al. 2021

J of Chemical Tech & Biotech

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“…The only pathway that was considerably under-expressed in the three experimental feeds compared to FO is peptidoglycan biosynthesis. The cell wall of some microalgae species contains peptidoglycan (Agboola et al 2019, Machado et al 2022), but the species used in our research do not seem to contain it (Domozych et al 2012, Le Costaouëc et al 2017, Nadzir et al 2023. Peptidoglycan forms around 90% of the dry weight of Grampositive bacteria but only 10% of Gram-negative strains (Malanovic & Lohner 2016).…”

Section: Discussionmentioning

confidence: 73%

The effect of dietary fish oil replacement by microalgae on the gilthead sea bream midgut bacterial microbiota

Katsoulis-Dimitriou,

Nikouli,

Gkalogianni

et al. 2024

Preprint

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The aim of this work was to evaluate the effects of dietary microalgae blends as fish oil replacers οn the midgut bacterial microbiota of gilthead sea bream (Sparus aurata). The control diet (FO) contained only fish oil as source of lipids, EPA and DHA fatty acids, while three experimental diets were used where fish oil was replaced at 67% by one of the following microalgae biomass blends: Michrochloropsis gaditana and Isochrysis sp. (MI), Phaeodactylum tricornutum and Isochrysis sp. (PI) and Schizochytrium sp. and P. tricornutum (SP). The midgut bacterial community composition and the dominant OTUs indicated that the sea bream midgut bacterial communities were altered compared to the control feed. Additional evidence from the presumptive bacterial functional pathways suggests that the microalgal-containing aquafeed resulted in one overexpressed and one underexpressed pathway. The overexpressed pathway was related to the metabolism of fucose, a major carbohydrate of these microalgae species. This suggests that a new gut microbiota profile was selected because of the microalgae inclusion in the provided aquafeed. All these data combined with the absence of mortality in fish, shows that the gilthead sea bream gut microbiome can smoothly adapt its function according to the metabolic capacity of the microalgae combinations that we used. The MI feed seems to promote several beneficial bacteria with potential probiotic abilities in the fish gut.

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“…Nevertheless, in some circumstances, chitinases can also interact and degrade cellulose polymers, and this might be the case for the chloroviruses GHs. [44][45][46] Chloroviruses are nucleocytoplasmic large DNA viruses (NCLDV, Nucleocytoviricota phylum) from the Phycodnaviridae family that infect unicellular zoochlorella. 47 Giant viruses have large genomes, with a wide range of genes capable of coding diverse proteins, including those related to DNA repair, transcription factors, and even metabolic enzymes.…”

Section: Chitinases In the Virospherementioning

confidence: 99%

An overview of viral chitinases: General properties and biotechnological potential

Oliveira,

Filho,

Rodrigues

2023

Exp Biol Med (Maywood)

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Chitin is a biopolymer profusely present in nature and of pivotal importance as a structural component in cells. It is degraded by chitinases, enzymes naturally produced by different organisms. Chitinases are proteins enrolled in many cellular mechanisms, including the remodeling process of the fungal cell wall, the cell growth process, the autolysis of filamentous fungi, and cell separation of yeasts, among others. These enzymes also have properties with different biotechnological applications. They are used to produce polymers, for biological control, biofilm formation, and as antitumor and anti-inflammatory target molecules. Chitinases are classified into different glycoside hydrolase (GH) families and are widespread in microorganisms, including viruses. Among them, the GH18 family is highly predominant in the viral genomes, being present and active enzymes in baculoviruses and nucleocytoplasmic large DNA viruses (NCLDV), especially chloroviruses from the Phycodnaviridae family. These viral enzymes contain one or more GH domains and seem to be involved during the viral replication cycle. Curiously, only a few DNA viruses have these enzymes, and studying their properties could be a key feature for biological and biotechnological novelties. Here, we provide an overview of viral chitinases and their probable function in viral infection, showing evidence of at least two distinct origins for these enzymes. Finally, we discuss how these enzymes can be applied as biotechnological tools and what one can expect for the coming years on these GHs.

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Optimisation of carbohydrate, lipid and biomass productivity inTetradesmus obliquususing response surface methodology

Cited by 8 publications

References 36 publications

Bioresource potential of Tetradesmus obliquusUJEA_AD: critical evaluation of biosequestration rate, biochemical and fatty acid composition in BG11 media

Bioresource potential of Tetradesmus obliquusUJEA_AD: critical evaluation of biosequestration rate, biochemical and fatty acid composition in BG11 media

The effect of dietary fish oil replacement by microalgae on the gilthead sea bream midgut bacterial microbiota

An overview of viral chitinases: General properties and biotechnological potential

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