Optimisation of Culture Conditions for PLA-food-packaging Degradation by Bacillus sp. SNRUSA4

Sawiphak, Suwapha; Wongjiratthiti, Aroon

doi:10.47836/pjst.29.1.23

Cited by 2 publications

(1 citation statement)

References 41 publications

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“…The recent study evaluated the PLA food packaging degradation by Bacillus sp. SNRUSA4 by using RSM approach and obtained the Box-Behnken design for the optimization studies (Sawiphak and Wongjiratthiti, 2021).…”

Section: Response Model Of Predictionmentioning

confidence: 99%

Isolation, characterization and optimization of bacterial isolate SARR1 for biodegradation of pretreated low density polyethylene

Rani

Jitender

Singh

et al. 2021

JANS

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Accumulation of low-density polyethylene (LDPE) has caused a threat to the environment because of its stable and inert nature as it cannot be degraded easily by microorganisms. Its lightweight, low cost, strength, durability, and its various other applications, have led to the wide usage of the polymer, which is exerting a negative effect on both marine and land biota. The development of an eco-friendly or a promising strategy is needed to reduce LDPE waste from both land and water. In the present study, observations have been made to isolate highly efficient LDPE degrading bacteria. The response surface methodology (RSM) was used to predict the best optimization of media for the degradation of LDPE by isolate SARR1. The isolate SARR1 was selected through primary screening by weight loss method and secondary screening using CO2 evolution test, TTC and MATH Test. The isolate SARR1 showed 6.30 ± 0.25 g/L CO2 evolution. The microbial adhesion hydrophobicity (MATH) was observed during log phase (100 to 56.89 ± 0.97 %) and stationary phase (100 to 82.92 ± 1.24 %). An isolate SARR1 converted the TTC into red coloured insoluble triphenyl formazan (TPF) after incubation of 7 days. The isolated bacteria SARR1 showed 38.3 ± 1.27 % biodegradation efficiency in the pretreated LDPE strips at 37 °C and pH 7.0 under optimized conditions within 30 days of incubation. This bioremediation and biodegradation approach is eco-friendly and safe for the environment. The results of treatment with isolate SARR1 had a potential hope to degrade LDPE at higher rate than natural degradation.

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Section: Response Model Of Predictionmentioning

confidence: 99%

Isolation, characterization and optimization of bacterial isolate SARR1 for biodegradation of pretreated low density polyethylene

Rani

Jitender

Singh

et al. 2021

JANS

View full text Add to dashboard Cite

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Statistical optimization of process variables for improved Polyethylene terephthalate plastic degradation by a rhizospheric bacterial consortium

Dhaka,

Singh,

Rao

et al. 2024

Preprint

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The current study focuses on the polyethylene terephthalate (PET) powder degradation potential of a rhizobacterial consortium screened from the rhizosphere of plants growing at plastic-polluted sites. The rhizobacterial isolates were screened, and the ability of PET powder degradation was studied for up to 18 days. For observing the efficiency of degradation, all three rhizobacterial strains with the highest percentage of degradation were combined to formulate the consortium. The Response Surface Methodology was used to optimize the process variables. The combinations demonstrating the highest weight reduction percentage for PET were selected for further degradation studies. The changes in the structure and surfaces that occurred after biodegradation on the plastic were observed through SEM and FTIR analysis. The obtained results showed the disappearance and elongation of the peak, signifying that the rhizobacterial consortium could modify the PET plastic. The weight reduction percentage of PET powder (300 µm) was 71.12% at optimized conditions (29.8ºC, 7.02 pH and 1g/L carbon source). The mathematical model developed through RSM is found to be significant (P < 0.05), and optimization and validation experiments were also well correlated for the process.

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Optimisation of Culture Conditions for PLA-food-packaging Degradation by Bacillus sp. SNRUSA4

Cited by 2 publications

References 41 publications

Isolation, characterization and optimization of bacterial isolate SARR1 for biodegradation of pretreated low density polyethylene

Isolation, characterization and optimization of bacterial isolate SARR1 for biodegradation of pretreated low density polyethylene

Statistical optimization of process variables for improved Polyethylene terephthalate plastic degradation by a rhizospheric bacterial consortium

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