Optimisation of enzymatic production of sulforaphane in broccoli sprouts and their total antioxidant activity at different growth and storage days

Tian, Ming; Xu, Xiaoyun; Hu, Hao; Liu, Yu; Pan, Siyi

doi:10.1007/s13197-016-2452-0

Cited by 20 publications

(18 citation statements)

References 37 publications

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“…Broccoli ( Brassica oleracea L. var. italica ) contains multiple nutrients (i.e., vitamins and minerals) and many health beneficial compounds, especially sulfur-containing metabolites ( Vasanthi et al, 2009 ; Tian et al, 2016a ). As an S and/or Se secondary accumulator, broccoli is able to accumulate high levels of S and Se when grown in S and Se rich environments.…”

Section: Introductionmentioning

confidence: 99%

Selenium-Induced Toxicity Is Counteracted by Sulfur in Broccoli (Brassica oleracea L. var. italica)

et al. 2017

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Selenium (Se) is an essential micronutrient for humans. Increasing Se content in food crops offers an effective approach to enhance the consumption of Se in human diets. A thoroughly understanding of the effects of Se on plant growth is important for Se biofortification in food crops. Given that Se is an analog of sulfur (S) and can be toxic to plants, its effect on plant growth is expected to be greatly affected by S nutrition. However, this remains to be further understood. Here, we evaluated the influence of Se treatments on broccoli (Brassica oleracea L. var. italica) growth when S was withheld from the growth nutrient solution. We found that Se was highly toxic to plants when S nutrition was poor. In contrast to Se treatments with adequate S nutrition that slightly reduced broccoli growth, the same concentration of Se treatments without S supplementation dramatically reduced plant sizes. Higher Se toxicity was observed with selenate than selenite under low S nutrition. We examined the bases underlying the toxicity. We discovered that the high Se toxicity in low S nutrition was specifically associated with an increased ratio of Se in proteins verse total Se level, enhanced generation of reactive oxygen species, elevated lipid peroxidation causing increased cell membrane damage, and reduced antioxidant enzyme activities. Se toxicity could be counteracted with increased supplementation of S, which is likely through decreasing non-specific integration of Se into proteins and altering the redox system. The present study provides information for better understanding of Se toxicity and shows that adequate S nutrition is important to prevent Se toxicity during biofortification of crops by Se fertilization.

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Section: Introductionmentioning

confidence: 99%

Selenium-Induced Toxicity Is Counteracted by Sulfur in Broccoli (Brassica oleracea L. var. italica)

et al. 2017

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“…China's "Internet + Agriculture" is starting from agricultural e-commerce and it will promote the development of agricultural e-commerce [3]. Therefore, studying the development model of agricultural e-commerce in the new environment has important theoretical and practical value [4]. Mobile Internet technology, cloud computing technology, and big data processing technology provides a guarantee for the implementation of agricultural e-commerce [5].…”

Section: Prefacementioning

confidence: 99%

An Empirical Study on the Relationship between Agricultural Science and Technology Input and Agricultural Economic Growth Based on E-Commerce Model

Wang

Huang

2018

Sustainability

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At present, e-commerce mode has been gradually applied to agricultural science and technology production, which has played an important role in agricultural economic growth and production efficiency. At the same time, the fundamental way out for sustainable and stable development of agriculture is science and technology. Generally speaking, part of the growth of the agricultural economy comes from agricultural production factors. The increase in input is partly due to the improvement of productivity of agricultural elements. Therefore, based on the background of the e-commerce environment, this paper chooses the entropy method to study the relationship between agricultural science and technology input and agricultural economic growth. Compared with the exponential method and the Bolat method, the entropy method can scientifically determine the specific weight of the indicators based on the variation of each quantitative index, so as to improve the accuracy and objectivity of the quantitative index analysis and avoid the adverse effects of human factors. The entropy method is used to evaluate and analyze the development level of agricultural e-commerce, which improves the accuracy and reliability of the evaluation results. Based on this, this paper makes an empirical study on the relationship between agricultural science and technology input and agricultural economic growth by using the method of entropy under the mode of e-commerce, constructs the index system of agricultural productivity, evaluates the situation of agricultural science and technology input and agricultural economic growth, and studies the relationship between them by using the method of regression analysis. Research shows that the application of agricultural science and technology investment and e-commerce mode can promote agricultural economic growth.

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“…Myrosinase in broccoli was shown to have the highest activity at pH 6.5–7 [23]. Ascorbic acid has been reported to act as a cofactor for myrosinase and higher enzyme activity was observed in the presence of ascorbic acid [24,25,26,27]. Furthermore, the formation of isothiocyanates is favored at neutral pH, while nitrile formation occurs under more acidic environments [28,29,30].…”

Section: Introductionmentioning

confidence: 99%

Formulation of Broccoli Sprout Powder in Gastro-Resistant Capsules Protects against the Acidic pH of the Stomach In Vitro but Does Not Increase Isothiocyanate Bioavailability In Vivo

et al. 2019

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Broccoli sprout powder is a rich source of glucosinolates, which are hydrolysed to isothiocyanates in the presence of the enzyme myrosinase. We showed that in vitro incubation of broccoli sprout powder extract with isolated lymphocytes resulted in the upregulation of transcription factor Nrf2, however, there was no increase in Nrf2 protein levels in lymphocytes isolated 3 h following the ingestion of broccoli sprout powder by healthy volunteers. This highlights the general issue that potential health benefits of food-derived compounds can be compromised by limitations in bioavailability. In vitro experiments showed that the generation of isothiocyanates was reduced when the powder was first exposed to the low pH (1.2) of the stomach and then transferred to the higher pH (6.8) of the intestine. The loss of activity due to pre-exposure to the low stomach pH indicates that formulating the broccoli sprout powder in gastro-resistant formulations should increase that amount of isothiocyanate generated in the intestine for absorption. Gelatin capsules were hand-coated with either Eudragit® L100 or Eudragit® L100-55 and were assessed for their gastro-resistant properties using paracetamol as a model active for dissolution studies. Disintegration and dissolution studies showed that Eudragit® L100-55 coated capsules and DRcapsTM (Capsugel®) failed the United States Pharmacopeia (USP) requirements for gastro-resistant capsules, whereas the Eudragit® L100 coated capsules passed. Five healthy participants were administered 1 g of broccoli sprout powder, ingested either with water or encapsulated in uncoated or gastro-resistant capsules. Urinary excretion of isothiocyanate metabolites over the 24 h period post ingestion was assessed by HPLC. Broccoli sprout powder and uncoated gelatin-encapsulated powder showed comparable excretion of isothiocyanate metabolites (18.4 ± 2.3 and 23.9 ± 2.7 µmol, respectively). The enteric coated capsules provided a significantly longer Tmax than the uncoated gelatin capsules (15.4 ± 2.3 versus 3.7 ± 0.7 h, respectively), indicating protection from disintegration in the stomach, however, the excretion of isothiocyanate metabolites was significantly decreased compared with uncoated capsules (i.e., 8.5 ± 1.1 µmol). The lower in vivo formation or absorption of isothiocyanates observed for the gastro-resistant capsules may be due to participant variation in intestinal pH or transit times, resulting in inappropriate pH conditions or insufficient time for the complete disintegration and dissolution of the capsules.

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Optimisation of enzymatic production of sulforaphane in broccoli sprouts and their total antioxidant activity at different growth and storage days

Cited by 20 publications

References 37 publications

Selenium-Induced Toxicity Is Counteracted by Sulfur in Broccoli (Brassica oleracea L. var. italica)

Selenium-Induced Toxicity Is Counteracted by Sulfur in Broccoli (Brassica oleracea L. var. italica)

An Empirical Study on the Relationship between Agricultural Science and Technology Input and Agricultural Economic Growth Based on E-Commerce Model

Formulation of Broccoli Sprout Powder in Gastro-Resistant Capsules Protects against the Acidic pH of the Stomach In Vitro but Does Not Increase Isothiocyanate Bioavailability In Vivo

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