Optimisation of glutamic acid production from cassava starch factory residues using Brevibacterium divaricatum

Jyothi, Alummoottil N.; Sasikiran, K.; Nambisan, Bala; Balagopalan, C.

doi:10.1016/j.procbio.2005.03.046

Cited by 68 publications

(39 citation statements)

References 4 publications

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“…A study conducted using Bacillus licheniformis showed that increasing inoculum size from 0.5 to 10 g/L increased volumetric 2,3-BD productivity and 2,3-BD yield from~0.04 to 0.35 g/L/h and 0.11 to 0.35 g/g, respectively [19]. Furthermore, Jyothi et al [29] obtained higher glutamic acid concentrations when the inoculum size of Brevibacterium divaricatum was increased from 3% to 7% with concomitant higher glutamic acid yields.…”

Section: Plackett-burman Designmentioning

confidence: 95%

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

et al. 2017

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While chiral 2,3-Butanediol (2,3-BD) is currently receiving remarkable attention because of its numerous industrial applications in the synthetic rubber, bioplastics, cosmetics, and flavor industries, 2,3-BD-mediated feedback inhibition of Paenibacillus polymyxa DSM 365 limits the accumulation of higher concentrations of 2,3-BD in the bioreactor during fermentation. The Box-Behnken design, Plackett-Burman design (PBD), and response surface methodology were employed to evaluate the impacts of seven factors including tryptone, yeast extract, ammonium acetate, ammonium sulfate, glycerol concentrations, temperature, and inoculum size on 2,3-butanediol (2,3-BD) production by Paenibacillus polymyxa DSM 365. Results showed that three factors; tryptone, temperature, and inoculum size significantly influence 2,3-BD production (p < 0.05) by P. polymyxa. The optimal levels of tryptone, inoculum size, and temperature as determined by the Box-Behnken design and response surface methodology were 3.5 g/L, 9.5%, and 35 • C, respectively. The optimized process was validated in batch and fed-batch fermentations in a 5-L Bioflo 3000 Bioreactor, and 51.10 and 68.54 g/L 2,3-BD were obtained, respectively. Interestingly, the production of exopolysaccharides (EPS), an undesirable co-product, was reduced by 19% when compared to the control. These results underscore an interplay between medium components and fermentation conditions, leading to increased 2,3-BD production and decreased EPS production by P. polymyxa. Collectively, our findings demonstrate both increased 2,3-BD titer, a fundamental prerequisite to the potential commercialization of fermentative 2,3-BD production using renewable feedstocks, and reduced flux of carbons towards undesirable EPS production.

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Section: Plackett-burman Designmentioning

confidence: 95%

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

et al. 2017

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“…Over production of glutamate by C. glutamicum is induced by biotin limitation (Gutmann et al, 1992), or by adding specific detergents (Eggeling et al, 2001;Amin and AlTalhi, 2007) or by adding sublethal amounts of penicillin in early exponential growth phase (Numheimer et al, 1970) or at last, by a temperature up-shift of the culture broth (Delaunay et al, 1999). Glucose, and other carbon source as, beet molasses (Yoshikiro et al, 1979) and cassava residues (Jyothi et al, 2005). Later on, an investigation carried out by other agriculture wastes which are the cheap carbon source such as Muntingia calabura L. are used by Vijayalakshmi and Sarvamangala (2011) for the production of glutamic acid.…”

Section: Introductionmentioning

confidence: 99%

Production of glutamic acid by Corynebacterium glutamicum using dates syrup as carbon source

Ahmed¹,

M²,

Khan³

et al. 2013

Afr. J. Microbiol. Res.

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The present study was an illustrative investigation on L-glutamic acid production by using Corynebacterium glutamicum employing a cheaply available dates syrup. Owing to the high sugar content of dates syrup (total sugar 80%) attempt was made to utilize the date syrup for the production of glutamic acid, in shaking culture. The acid treated dates syrup (ATDS) at concentration of 100g/L was the best sugar sources in the glutamic production. Penicillin addition at concentration of 4 U/ml after 12 h of incubation was superior in glutamic acid production. The selected temperature-sensitive mutants M5AJ2, showed 13.4% increase in glutamic production while, M5AJ4 and M7AJ6 showed 22.4 and 4.6% decrease respectively, in their glutamic acid production than their wild type bacterial strain. The specific production rate of glutamic acid in case of temperature shift-up from 31 to 39°C increased apparently 2 and 1.5 fold respectively on average from that under the constant temperature. 24g/L pure glutamic acid were precipitated in crystal at pH 3.2.

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“…In India, more than 1500 cottage and small scale industries crush over 5000 tonnes of cassava per day during harvest season (Edison et al, 2006). In the extraction of starch from cassava, fibrous residue, called as cassava fibrous residue (CFR) constitute about 15-20% by weight of cassava chips/ tubers processed, is retained on the sieves during the rasping process, which contain about 10-15% crude fiber and 55-65% starch (on dry weight basis) (Ray, 2004;Jyothi et al, 2005). However, there is serious concern about the disposal of solid residue left after the large-scale extraction of starch from cassava.…”

Section: Introductionmentioning

confidence: 99%

“…However, there is serious concern about the disposal of solid residue left after the large-scale extraction of starch from cassava. Due to its rich organic matter and low ash content (Pandey et al, 2000a,c), it can serve as an ideal substrate for microbial processes for the production of value added products, i.e., organic acid (Jyothi et al, 2005), ethanol and enzyme , etc. Among the various extracellular thermostable enzymes, α-amylase ranks first in terms of commercial exploitation (Pandey et al, 2000a;Haki and Rakshit, 2003), particularly in starch processing industry.…”

Section: Introductionmentioning

confidence: 99%

Optimization of thermostable α- amylase production by Streptomyces erumpens MTCC 7317 in solid-state fermentation using cassava fibrous residue

Kar

Datta²,

Ray

2010

Braz. arch. biol. technol.

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Optimisation of glutamic acid production from cassava starch factory residues using Brevibacterium divaricatum

Cited by 68 publications

References 4 publications

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

Process Development for Enhanced 2,3-Butanediol Production by Paenibacillus polymyxa DSM 365

Production of glutamic acid by Corynebacterium glutamicum using dates syrup as carbon source

Optimization of thermostable α- amylase production by Streptomyces erumpens MTCC 7317 in solid-state fermentation using cassava fibrous residue

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