Optimisation of hydrolysis conditions and fractionation of peptide cryoprotectants from gelatin hydrolysate

Profiles of Enzymatic Hydrolysis of Different Collagens and Derivatives Over Time

“…For industrial production purposes, gelatin is obtained from collagen using acid or alkaline hydrolysis. It is a substance widely used as a GAREIS, 2007, WANG et al, 2009). …”

Physicochemical properties of skin gelatin from farmed Amur sturgeon (Acipenser schrenckii) as influenced by acid pretreatment

“…It contains relatively high amounts of amino acids such as glycine, proline, hydroxyproline, and alanine (Wang, Agyare, & Damodaran, 2009). Tropocollagen is the basic unit of collage and consists of three polypeptide chains with identical or different amino acid sequence (Strasburg, Xiong, & Chiang, 2007).…”

Introduction to Frozen Food Packaging

“…In the primary structure of collagen, where the first position of this tripeptide repeat is always occupied by Gly, the second position is not always occupied by Pro or Hyp. Thus, in addition to -Gly-Pro-X segments, there are several segments with sequences -Gly-Z-X-(where Z is any amino acid residue) within the collagen sequence [47]. Previously, it was reported that enzymatic modification of gelatin with l-leucine n-dedecyl ester through the plastein reaction using papain resulted in a product that exhibited antifreeze properties [47,48].…”

“…Thus, in addition to -Gly-Pro-X segments, there are several segments with sequences -Gly-Z-X-(where Z is any amino acid residue) within the collagen sequence [47]. Previously, it was reported that enzymatic modification of gelatin with l-leucine n-dedecyl ester through the plastein reaction using papain resulted in a product that exhibited antifreeze properties [47,48]. The l-leucine n-dodecyl ester derivative of gelatin hydrolysate was found to decrease the freezing point of water to about −7°C in the presence of the heterogeneous nucleation inducer AgI, whereas the control hydrolysate decreased the freezing point only to about −4°C.…”