Optimisation of the coupled monoclonal antibody density for recombinant hepatitis B virus surface antigen immunopurification

Purification process development for HER1 extracellular domain as a potential therapeutic vaccine

“…The monoclonal antibody anti hEGFR (supplied by the Center for Molecular Immunology) immobilization was developed according to the previously described procedure [12]. Coupling efficiency ( ) for 5 mg/mL ligand density was determined by an indirect method, following the formula:…”

Molecular imprinting based composite cryogel membranes for purification of anti-hepatitis B surface antibody by fast protein liquid chromatography

“…The purification system was mainly based on protein A affinity chromatography used widely for antibody purification protocols [9][10][11][12][13]. Hernandez et al purified anti-HBs from ascites fluid of immunized mice by Sepharose CL-4B-protein A adsorbent [9]. Mouta Junior et al purified anti-HBs polyclonal and monoclonal antibodies by Affi-Prep protein A MAPS II [10].…”

Hepatitis B surface antibody purification with hepatitis B surface antibody imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-l-tyrosine methyl ester) particles

“…The purification system was mainly based on protein A affinity chromatography, which has been widely used for hepatitis B surface antibody (HBsAb) purification [24][25][26][27][28]. Hernandez et al purified HBsAb from ascites fluid of immunized mice by Sepharose CL-4B-protein A adsorbent [24].…”

“…Hernandez et al purified HBsAb from ascites fluid of immunized mice by Sepharose CL-4B-protein A adsorbent [24]. Mouta Junior et al purified antiHBs polyclonal and monoclonal antibodies by Affi-Prep protein A MAPS II [25].…”