Optimisation of the tissue culture protocol for the endangered Aloe polyphylla

Chukwujekwu, J.C.; Fennell, Catherine; Staden, J. Van

doi:10.1016/s0254-6299(15)30368-9

Cited by 26 publications

(22 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typically, shoots obtained from these treatments were fragile, stunted and had a pale green colour. Chukwujekwu et al (2002) also reported that on MS medium (20.6 mM NH 4 + ) high levels of BA or zeatin did not improve shoot multiplication of A. polyphylla. Raising the concentration of exogenous BA and zeatin in the MS medium (20.6 mM NH 4 + ) had a positive effect on the accumulation of endogenous CKs in A. polyphylla shoots (Ivanova et al 2006), however this did not translate into a high multiplication rate.…”

Section: Anovamentioning

confidence: 97%

See 1 more Smart Citation

Effect of ammonium ions and cytokinins on hyperhydricity and multiplication rate of in vitro regenerated shoots of Aloe polyphylla

Ivanova

Staden

2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

In search for the optimal culture conditions resulting in a high production of healthy plants and low occurrence of hyperhydricity in tissue cultured regenerants of Aloe polyphylla, we investigated the relationship between ammonium ions in the medium, applied cytokinins (CKs) and CK concentrations in the induction of hyperhydricity. Shoots were grown on media with different NH 4 + concentrations (10.3, 20.6 and 61.8 mM) and supplemented with N 6 -benzyladenine (BA), zeatin or thidiazuron (TDZ) at 0, 5 or 15 lM. Elevating the levels of NH 4 + , in the absence of CKs, could not induce hyperhydricity. Similarly, very low hyperhydricity was observed when CKs were added to media containing low NH 4 + (10.3 mM). However, in the presence of higher NH 4 + concentrations, CKs increased hyperhydricity in a concentration-dependant manner, suggesting that they were capable of inducing this syndrome only when other factors in the culture system were not optimised. High numbers of healthy looking shoots were produced on media with low NH 4 + and low BA or zeatin (5 lM). The use of TDZ resulted in the formation of buds, which did not develop into shoots. Identifying the factors responsible for hyperhydricity is an important step in the successful use of the micropropagation technique for the conservation of this species.

show abstract

Section: Anovamentioning

confidence: 97%

“…Applying the commonly used benzyladenine (BA) resulted in a high multiplication rate, but the quality and survival of the shoots were impaired by the occurrence of hyperhydricity. The use of zeatin was suggested for avoiding HS (Chukwujekwu et al 2002). However, the high cost of zeatin increases the price of the final product.…”

Section: Anovamentioning

confidence: 99%

Effect of ammonium ions and cytokinins on hyperhydricity and multiplication rate of in vitro regenerated shoots of Aloe polyphylla

Ivanova

Staden

2007

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Bulking of stock material was done using full strength MS media (Murashige and Skoog 1962) supplemented with 30 gl À1 sucrose, 0.1 g l À1 myo-inositol, 0.5 mg l À1 IBA, 1.0 mg l À1 zeatin and solidified with 1% agar (Bacteriological agar-Oxoid Ltd., Basingstoke, Hampshire, England). Cultures were incubated in a growth room with continuous cool fluorescent tubes (Osram L75 W/20X) light intensity of 45 mmol m À2 s À1 and a temperature of 24 ± 18C for 9 weeks (Chukwujechwu et al 2002).…”

Section: Source Materials and Bulking Of Explantsmentioning

confidence: 99%

“…It grows in basalt rock crevices on high altitude slopes (2230-2720 m) and is the only alpine member of the genus Aloe. It thrives well in loose rocks that facilitate good drainage (Aubrey 2002;Chukwujechwu et al 2002). This plant is grown mainly for horticultural (ornamental) and medicinal purposes.…”

Section: Introductionmentioning

confidence: 99%

“…Cooperative efforts between conservationists and nurseries to propagate the plant for commercial trade is having some success in reducing the number of spiral aloes collected from its wild habitat (Aubrey 2002). To ensure conservation of this endangered species a micropropagation protocol has been developed (Abrie and van Staden 2001; Chukwujechwu et al 2002). Incidence of hyperhydricity, the need for an additional rooting step and the use of zeatin (a very expensive cytokinin) are limitations of this protocol.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin?

Bairu

Stirk

Doležal

et al. 2007

Plant Cell Tiss Organ Cult

185

View full text Add to dashboard Cite

Benzyladenine (BA) is the most widely used cytokinin in the micropropagation industry due to its effectiveness and affordability. It, however, has disadvantages such as genetic alteration and abnormal growth in some plants. Naturally occurring zeatin on the other hand is not as widely used as BA and is far more expensive. The use of meta-topolin and its derivatives as alternatives to BA and zeatin, both of which frequently have negative effects in tissue culture was investigated. In vitro grown Aloe polyphylla (an endangered medicinal and ornamental aloe) were cultured on full strength Murashige and Skoog basal medium with different concentrations of cytokinins and solidified with 1% Bacteriological Agar (Oxoid No. 1). mT was the preferred cytokinin both in terms of multiplication rate and rooting. The optimum concentration that induced regeneration and rooting is 5.0 mM. The problem of hyperhydricity was totally controlled. Plants rooted spontaneously in multiplication medium, thus avoiding the extra rooting step of the protocol. More than 91% of the plants transferred to ex vitro conditions were successfully acclimatized.

show abstract