Optimising conditions for growth and xanthophyll production in continuous culture of <i>Tisochrysis lutea</i> using photobioreactor arrays and central composite design experiments

Beuzenberg, Veronica; Goodwin, Eric; Puddick, Jonathan; Romanazzi, Donato; Adams, Serean L.; Packer, Michael A.

doi:10.1080/0028825x.2016.1238398

Cited by 12 publications

(7 citation statements)

References 23 publications

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“…They were assessed further for eligibility based on the defined criteria: (1) inclusion of studies, specifically fucoxanthin production of microalgae involving fucoxanthin content or both growth (biomass concentration) and fucoxanthin content under different culture conditions, and the (2) exclusion of studies involving environmental studies (studies utilised fucoxanthin to analyse the microalgae community in a particular habitat). Finally, 28 articles were included in the present systematic review [ 4 , 5 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. The information from these articles was extracted and is summarised in Table 1 .…”

Section: Resultsmentioning

confidence: 99%

“…Like salinity, each fucoxanthin-producing microalga has its optimum temperature to yield the maximum growth and fucoxanthin content. An optimisation study employing response surface methodology has shown that the optimum temperature for T. lutea to generate the greatest fucoxanthin content was 25 °C [ 24 ]. In the other study, the same microalgae, T. lutea , leaned towards the temperature of 30 °C among the other temperatures (16.5, 20 and 25 °C), with which the peak biomass concentration (1.81 g/L) and fucoxanthin content (around 0.21% DW) were obtained [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

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Fucoxanthin Production of Microalgae under Different Culture Factors: A Systematic Review

et al. 2022

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Fucoxanthin is one of the light-harvesting pigments in brown microalgae, which is increasingly gaining attention due to its numerous health-promoting properties. Currently, the production of microalgal fucoxanthin is not yet feasible from an economic perspective. However, the cultivation of microalgae at favourable conditions holds great potential to increase the viability of this fucoxanthin source. Hence, this study aimed to review the fucoxanthin production of microalgae under different conditions systematically. A literature search was performed using the Web of Science, Scopus and PubMed databases. A total of 188 articles were downloaded and 28 articles were selected for the current review by two independent authors. Microalgae appeared to be a more reliable fucoxanthin source compared to macroalgae. Overall, a consensus fucoxanthin production condition was obtained and proposed: light intensity ranging from 10 to 100 µmol/m2/s could achieve a higher fucoxanthin content. However, the optimal light condition in producing fucoxanthin is species-specific. The current review serves as an antecedent by offering insights into the fucoxanthin-producing microalgae response to different culture factors via a systematic analysis. With the current findings and recommendations, the feasibility of producing fucoxanthin commercially could be enhanced and possibly achieve practical and sustainable fucoxanthin production.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fucoxanthin Production of Microalgae under Different Culture Factors: A Systematic Review

et al. 2022

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“…Carotenoids are well-known dietary supplements used as ingredients in food and cosmeceutical formulations (e.g., as colorants), and perform versatile roles in human health, including neuroprotection and the prevention and/or treatment of macular degeneration, inflammatory and rheumatoid arthritis, cataracts, cancer, diabetes, and coronary artery conditions, among others [35]. Both pigments, fucoxanthin and zeaxanthin, have been reported in Tisochrysis lutea [36], which might explain the presence of those monoterpene lactone diastereomers in this marine microalga. Indeed, carotenoids are highly prone to oxidation under different environments, such as light, heat, air, or the presence of transition metals or radicals.…”

Section: Resultsmentioning

confidence: 99%

Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis Lutea

et al. 2020

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Tisochrysis lutea is a marine haptophyte rich in omega-3 polyunsaturated fatty acids (e.g., docosahexaenoic acid (DHA)) and carotenoids (e.g., fucoxanthin). Because of the nutraceutical applications of these compounds, this microalga is being used in aquaculture to feed oyster and shrimp larvae. In our earlier report, T. lutea organic crude extracts exhibited in vitro cytotoxic activity against human hepatocarcinoma (HepG2) cells. However, so far, the compound(s) accountable for the observed bioactivity have not been identified. Therefore, the aim of this study was to isolate and identify the chemical component(s) responsible for the bioactivity observed. Bioassay-guided fractionation through a combination of silica-gel column chromatography, followed by preparative thin layer chromatography (PTLC), led to the isolation of two diastereomers of a monoterpenoid lactone, namely, loliolide (1) and epi-loliolide (2), isolated for the first time in this species. The structural elucidation of both compounds was carried out by GC-MS and 1D (1H and 13C APT) and 2D (COSY, HMBC, HSQC-ed, and NOESY) NMR analysis. Both compounds significantly reduced the viability of HepG2 cells and were considerably less toxic towards a non-tumoral murine stromal (S17) cell line, although epi-loliolide was found to be more active than loliolide.

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“…The biosynthesis of diatom carotenoids is profoundly affected by the quality of the light [ 16 ]. Yet, the impact of light quality (light source, intensity, and photoperiod) on fucoxanthin production remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light

et al. 2018

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BackgroundMarine diatoms have a higher fucoxanthin content in comparison to macroalgae. Fucoxanthin features many potent bioactive properties, particularly anti-obesity properties. Despite the great potential for harvesting larger amounts of fucoxanthin, the impacts of light quality (light source, intensity, and photoperiod) on fucoxanthin production and the essential proteins involved in fucoxanthin biosynthesis in marine diatoms remain unclear.ResultsIn the present study, Cylindrotheca closterium was selected from four different species of diatoms based on its high fucoxanthin content and productivity. Optimal light conditions (light source, intensity, and regime) were determined by a “Design of Experiment” approach (software MODDE Pro 11 was used). The model indicated that an 18/6 light/darkness regime increased fucoxanthin productivity remarkably as opposed to a 12/12 or 24/0 regime. Eventually, blue light-emitting diode light, as an alternative to fluorescent light, at 100 μmol/m2/s and 18/6 light/darkness regime yielded maximum fucoxanthin productivity and minimal energy consumption. The fucoxanthin production of C. closterium under the predicted optimal light conditions was assessed both in bottle and bag photobioreactors (PBRs). The high fucoxanthin content (25.5 mg/g) obtained from bag PBRs demonstrated the feasibility of large-scale production. The proteomes of C. closterium under the most favorable and unfavorable fucoxanthin biosynthesis light/darkness regimes (18/6 and 24/0, respectively) were compared to identify the essential proteins associated with fucoxanthin accumulation by matrix-assisted laser desorption/ionization-time of flight–mass spectrometry. Six proteins that were up-regulated in the 18/6 regime but down-regulated in the 24/0 were identified as important chloroplastic proteins involved in photosynthesis, energy metabolism, and cellular processes.ConclusionsBlue light-emitting diode light at 100 μmol/m2/s and 18/6 light/darkness regime induced maximum fucoxanthin productivity in C. closterium and minimized energy consumption. The high fucoxanthin production in the bag photobioreactor under optimal light conditions demonstrated the possibility of commercialization. Proteomics suggests that fucoxanthin biosynthesis is intimately associated with the photosynthetic efficiency of the diatom, providing another technical and bioengineering outlook on fucoxanthin enhancement.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0957-0) contains supplementary material, which is available to authorized users.

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Optimising conditions for growth and xanthophyll production in continuous culture of Tisochrysis lutea using photobioreactor arrays and central composite design experiments

Cited by 12 publications

References 23 publications

Fucoxanthin Production of Microalgae under Different Culture Factors: A Systematic Review

Fucoxanthin Production of Microalgae under Different Culture Factors: A Systematic Review

Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis Lutea

Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light

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