Optimising the effect of activated carbon on the microrheological properties of erythrocyte in rats with experimentally developed obesity

Abstract

Background: Remains not fully clear the impact of intestinal chelators on microrheological violation of red blood cells during the early stages of obesity. It dictates the necessity of experimental investigations' fulfilment on laboratory animals with just developed obesity in them.Method: Ninety-four of healthy male-rats of Vistar line at the age of 2.5-3 months took into investigation. Twenty-nine animals of them had experienced no impacts and composed the control group. In 65 rats after their putting into small cages (one specimen - in a cage) during 30 days there was developed OB as the result of giving them of high-caloric diet from combined feed (47%), sweet condensed milk (44%), vegetable oil (8%) and vegetable starch (1%). Of the 35 rats received a day for 60 days of activated carbon 25 mg/kg into a stomach. There used biochemical, haematological and statistical methods of investigation.Results: At obesity development in rats, there found a steady decrease of erythrocytes-discocytes quantity in blood. It accompanied by increase of reversibly and irreversibly changed erythrocytes' quantity in examined animals' blood. At obesity development in rats there was found quick rise of erythrocytes' sum in aggregate and these aggregates' quantity at lowering of free erythrocytes' number. During experimental obesity modelling we noticed very early in rats' blood decrease of erythrocytes-discocytes' quantity, level rise of their reversibly and irreversibly varieties with strengthening of their aggregative ability. It takes place in the background of weakening of erythrocytes' antioxidant protection and activation of lipids' peroxidation in them.Conclusion: Application of activated carbons capable of rats with experimental obesity resolve arisen they breach microrheological properties of erythrocytes. It is clear that in obesity treatment regimens must be intestinal chelators, capable of positively affect many broken with this pathology haematological indices.

The Anti-Obesity Effect of Porous Silica Is Dependent on Pore Nanostructure, Particle Size, and Surface Chemistry in an In Vitro Digestion Model

“…The limitations linked with orlistat present opportunities for the development of new anti-obesity therapeutics that act locally within the GI tract to limit energy intake, but through a mechanism of action with limited side effects. In recent years, it has been hypothesized that porous colloid biomaterials (e.g., smectite clays [7,8], porous silica [9][10][11], activated carbon [12]) may serve as effective anti-obesity therapies through their capacity to adsorb large quantities of macronutrients within their porous matrices [13]. In contrast to orlistat's mechanism of action where the inhibition of lipase activity leads to the production of oily stools, porous colloids can inhibit lipase activity while also adsorbing remaining undigested fats within the GI tract, thus eliminating a large portion of the diarrhea and GI distress caused by inhibiting GI lipolysis [8].…”