Optimization and characterization of exopolysaccharide produced by Bacillus aerophilus rk1 and its in vitro antioxidant activities

Ravi, G.; Sampath, Gattu; Beduru, Srinivas; Sarika, Kasarla; Govindarajan, Rasiravathanahalli Kaveriyappan; Ameen, Fuad; Alwakeel, Suaad S.; Thampu, Raja Komuraiah

doi:10.1016/j.jksus.2021.101470

Cited by 38 publications

(15 citation statements)

References 37 publications

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“…The higher antioxidant activity (76–78%) was achieved in all cases at 5 mg mL −1 of EPS concentration. This good microbial EPS-mediated antioxidant activity was also reported previously for other neutral EPSs [ 78 , 79 ]. It has been reported that the antioxidant properties of EPSs are associated with the sugar composition of polysaccharides and their physicochemical properties [ 77 ].…”

Section: Discussionsupporting

confidence: 87%

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

et al. 2022

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Extremophilic microorganisms often produce novel bioactive compounds to survive under harsh environmental conditions. Exopolysaccharides (EPSs), a constitutive part of bacterial biofilm, are functional biopolymers that act as a protecting sheath to the extremophilic bacteria and are of high industrial value. In this study, we elucidate a new EPS produced by thermophilic Bacillus haynesii CamB6 from a slightly acidic (pH 5.82) Campanario hot spring (56.4 °C) located in the Central Andean Mountains of Chile. Physicochemical properties of the EPS were characterized by different techniques: Scanning electron microscopy- energy dispersive X-ray spectroscopy (SEM-EDS), Atomic Force Microscopy (AFM), High-Performance Liquid Chromatography (HPLC), Gel permeation chromatography (GPC), Fourier Transform Infrared Spectroscopy (FTIR), 1D and 2D Nuclear Magnetic Resonance (NMR), and Thermogravimetric analysis (TGA). The EPS demonstrated amorphous surface roughness composed of evenly distributed macromolecular lumps. GPC and HPLC analysis showed that the EPS is a low molecular weight heteropolymer composed of mannose (66%), glucose (20%), and galactose (14%). FTIR analysis demonstrated the polysaccharide nature (–OH groups, Acetyl groups, and pyranosic ring structure) and the presence of different glycosidic linkages among sugar residues, which was further confirmed by NMR spectroscopic analyses. Moreover, D-mannose α-(1→2) and α-(1→4) linkages prevail in the CamB6 EPS structure. TGA revealed the high thermal stability (240 °C) of the polysaccharide. The functional properties of the EPS were evaluated for food industry applications, specifically as an antioxidant and for its emulsification, water-holding (WHC), oil-holding (OHC), and flocculation capacities. The results suggest that the study EPS can be a useful additive for the food-processing industry.

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Section: Discussionsupporting

confidence: 87%

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

et al. 2022

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“…The DPPH free radical quenching activity of EPS has increased in a dose-dependent way, wherein the concentration of the EPS is directly proportional to the antioxidant activity. DPPH activity was comparatively higher than the previously reported EPSs isolated from Paenibacillus polymyxa EJS-3 (45.4%) and B. aerophilus rk1 (57.6%), wherein 4 mg mL −1 of the EPS was used for the DPPH assay (Liu et al, 2010;Gangalla et al, 2021). Furthermore, EPS exhibits the ABTS quenching activity of about 64.24 ± 1.53% at the maximum EPS concentration of 3.0 mg mL −1 , which is considerably lesser than L-ascorbic acid (85.99 ± 1.65%) (Figure 8B).…”

Section: Antioxidant Activitiesmentioning

confidence: 61%

Exopolysaccharide Produced by Probiotic Bacillus albus DM-15 Isolated From Ayurvedic Fermented Dasamoolarishta: Characterization, Antioxidant, and Anticancer Activities

et al. 2022

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An exopolysaccharide (EPS) was purified from the probiotic bacterium Bacillus albus DM-15, isolated from the Indian Ayurvedic traditional medicine Dasamoolarishta. Gas chromatography-mass spectrophotometry and nuclear magnetic resonance (NMR) analyses revealed the heteropolymeric nature of the purified EPS with monosaccharide units of glucose, galactose, xylose, and rhamnose. Size-exclusion chromatography had shown the molecular weight of the purified EPS as around 240 kDa. X-ray powder diffraction analysis confirmed the non-crystalline amorphous nature of the EPS. Furthermore, the purified EPS showed the maximum flocculation activity (72.80%) with kaolin clay and emulsification activity (67.04%) with xylene. In addition, the EPS exhibits significant antioxidant activities on DPPH (58.17 ± 0.054%), ABTS (70.47 ± 0.854%) and nitric oxide (58.92 ± 0.744%) radicals in a concentration-dependent way. Moreover, the EPS showed promising cytotoxic activity (20 ± 0.97 μg mL–1) against the lung carcinoma cells (A549), and subsequent cellular staining revealed apoptotic necrotic characters in damaged A549 cells. The EPS purified from the probiotic strain B. albus DM-15 can be further studied and exploited as a potential carbohydrate polymer in food, cosmetic, pharmaceutical, and biomedical applications.

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“…Although previous reports had explored the DPPH scavenging efficiency of EPS produced by Bacillus sp. (Hu et al, 2019;Gangalla et al, 2021). However, the scavenging efficiency of BPS was recorded to be higher at the same concentration.…”

Section: Antioxidant Activity Of Bpsmentioning

confidence: 90%

The structure, characterization and dual-activity of exopolysaccharide produced by Bacillus enclensis AP-4 from deep-sea sediments

Li²,

Zhang³

et al. 2022

Front. Mar. Sci.

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In recent years, the exopolysaccharide (EPS) produced by deep-sea bacteria has attracted the interest of various researchers. In the present study, we have explored the properties and structure of a novel exopolysaccharide (called BPS) produced by Bacillus enclensis AP-4 from deep-sea sediments. The maximum yield of BPS was 4.23 ± 0.17 g L−1 in a 2216E modified medium. 1H NMR studies of the purified BPS displayed α and β-configuration sugar residues, including mannose, glucosamine, glucose, galactose, and xylose in a molar ratio of 1.00: 0.09: 0.04: 0.09: 0.07. BPS showed a molecular weight of 23,434 Da and was abundant in hydroxyl and amino residues. In addition, BPS exhibited a rod-like structure with a rough surface and was dominated by C, N, and O elements. The exopolysaccharide demonstrated remarkable thermal stability, high degradation temperature, and excellent emulsification capacity compared to most reported exopolysaccharides. Moreover, BPS displayed better quenching activities against the four radicals, which provided favorable protection for the strain. Finally, the freezing experiment investigated the cryoprotective effect of BPS on E. coli and S. aureus. BPS effectively improved the cell survival ratio and maintained the activity of Na+/K+-ATPase, which facilitates culture preservation. To the best of our knowledge, our work is the first report suggesting that marine exopolysaccharide has dual-activity. This work presents the foundation for the analysis of the structure and properties of exopolysaccharides produced by deep-sea bacteria.

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Optimization and characterization of exopolysaccharide produced by Bacillus aerophilus rk1 and its in vitro antioxidant activities

Cited by 38 publications

References 37 publications

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

Optimization and Characterization of a Novel Exopolysaccharide from Bacillus haynesii CamB6 for Food Applications

Exopolysaccharide Produced by Probiotic Bacillus albus DM-15 Isolated From Ayurvedic Fermented Dasamoolarishta: Characterization, Antioxidant, and Anticancer Activities

The structure, characterization and dual-activity of exopolysaccharide produced by Bacillus enclensis AP-4 from deep-sea sediments

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