Optimization and characterization of PHA from isolate Pannonibacter phragmitetus ERC8 using glycerol waste

Ray, Sanket; Prajapati, Vimalkumar S.; Patel, K. C.; Trivedi, Ujjval

doi:10.1016/j.ijbiomac.2016.02.002

Cited by 54 publications

(18 citation statements)

References 25 publications

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“…Nevertheless, the polyesters produced by B. cepacia IPT 119 and 400 displayed thermal performances superior to what was observed in the results reported by Ribeiro et al (2016) and Ray et al (2016) for PHA synthetized by Bacillus megaterium and Pannonibacter phragmitetus, respectively.…”

Section: Pha Characterizationmentioning

confidence: 50%

“…As a result, the replacement of synthetic materials by biodegradable ones, at competitive costs, is an emerging need that must be fulfilled. A promising alternative to this demand is the production of bacterial biopolymers, especially the group formed by polyhydroxyalkanoates (PHA), since the referred materials can replace conventional thermoplastics (Ray et al, 2016;Faccin et al, 2013;Kourmentza et al 2017;Koller et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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ASSESSMENT OF POLYHYDROXYALKANOATE SYNTHESIS IN SUBMERGED CULTIVATION OF Cupriavidus necator AND Burkholderia cepacia STRAINS USING SOYBEAN AS SUBSTRATE

Rodrigues

Nunes

Lordelo

et al. 2019

Braz. J. Chem. Eng.

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Polyhydroxyalkanoates (PHA) are biocompatible and biodegradable polyesters produced by prokaryotic microbes for energy storage and carbon reserve. These polymers are an option to diminish the massive impact caused by inadequate disposal of synthetic plastics. In this study, evaluation and characterization of PHA produced by Cupriavidus necator (IPT 026 and IPT 027) and Burkholderia cepacia (IPT 119 and IPT 400), using soybean as substrate, were carried out (soybean 15 g L -1 , pH 7.0, 150 rpm, 72 hours). The highest polymer production was achieved using IPT 027 (0.84 ± 0.07 g L -1 ). All PHA produced showed the characteristic bands of polyester functional groups in the FTIR spectra. Polymers synthesized by Cupriavidus necator exhibited initial temperatures of degradation superior to 300oC and higher molecular weights than the ones produced by Burkholderia cepacia, which in turn, exhibited lower crystallinity (inferior to 30%), revealing high influence of the microorganism strain on PHA properties and production.

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Section: Pha Characterizationmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

ASSESSMENT OF POLYHYDROXYALKANOATE SYNTHESIS IN SUBMERGED CULTIVATION OF Cupriavidus necator AND Burkholderia cepacia STRAINS USING SOYBEAN AS SUBSTRATE

Rodrigues

Nunes

Lordelo

et al. 2019

Braz. J. Chem. Eng.

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“…At this point it should be highlighted that despite nitrogen 15 limitation increases PHAs production 41 , no nitrogen limitation was carried out in this study due to its negative effect on ectoine production, which has a higher profit margin than PHAs. Finally, the synthesis of PHA recorded during C3 in R1 could be directly attributed to the occurrence of the genera Brevundimonas and Pannonibacter under low Mg 2+ concentrations, which are well known polyhydroxybutyrate producers 42,43 .…”

Section: Ectoines Productionmentioning

confidence: 95%

Multi-production of high added market value metabolites from diluted methane emissions via methanotrophic extremophiles

Cantera

Sánchez‐Andrea

Lebrero

et al. 2018

Bioresource Technology

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This study constitutes the first-proof-of-concept of a methane biorefinery based on the multi-production of high profit margin substances (ectoine, hydroxyectoine, polyhydroxyalkanoates (PHAs) and exopolysaccharides (EPS)) using methane as the sole carbon and energy source. Two bubble column bioreactors were operated under different magnesium concentrations (0.2, 0.02 and 0.002 g L) to validate and optimize this innovative strategy for valorization of CH emissions. High Mg concentrations promoted the accumulation of ectoine (79.7-94.2 mg g biomass), together with high hydroxyectoine yields (up to 13 mg g biomass) and EPS concentrations (up to 2.6 g L culture broth). Unfortunately, PHA synthesis was almost negligible (14.3 mg L) and only found at the lowest Mg concentration tested. Halomonas, Marinobacter, Methylophaga and Methylomicrobium, previously described as ectoine producers, were dominant in both bioreactors, Methylomicrobium being the only described methanotroph. This study encourages further research on CH biorefineries capable of creating value out of GHG mitigation.

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“…It was realized that limiting the intracellular depolymerization by knocking out phaZ1 gene resulted in enhanced PHA yield [90]. Using response surface methodology for optimizing feed as carbon source, glycerol waste (0.85 v/v), with mixed wastes (0.26 % w/v) leads to 1.87 g/L of co-polymer of PHA: PHB and medium chain length 3-hydroxyalkanoates by Pannonibacter phragmitetus [91].…”

Section: Poly(hydroxyalkanoates)mentioning

confidence: 99%

Biorefinery for Glycerol Rich Biodiesel Industry Waste

2016

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The biodiesel industry has the potential to meet the fuel requirements in the future. A few inherent lacunae of this bioprocess are the effluent, which is 10 % of the actual product, and the fact that it is 85 % glycerol along with a few impurities. Biological treatments of wastes have been known as a dependable and economical direction of overseeing them and bring some value added products as well. A novel eco-biotechnological strategy employs metabolically diverse bacteria, which ensures higher reproducibility and economics. In this article, we have opined, which organisms and what bioproducts should be the focus, while exploiting glycerol as feed.

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Optimization and characterization of PHA from isolate Pannonibacter phragmitetus ERC8 using glycerol waste

Cited by 54 publications

References 25 publications

ASSESSMENT OF POLYHYDROXYALKANOATE SYNTHESIS IN SUBMERGED CULTIVATION OF Cupriavidus necator AND Burkholderia cepacia STRAINS USING SOYBEAN AS SUBSTRATE

ASSESSMENT OF POLYHYDROXYALKANOATE SYNTHESIS IN SUBMERGED CULTIVATION OF Cupriavidus necator AND Burkholderia cepacia STRAINS USING SOYBEAN AS SUBSTRATE

Multi-production of high added market value metabolites from diluted methane emissions via methanotrophic extremophiles

Biorefinery for Glycerol Rich Biodiesel Industry Waste

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