Optimization and Characterization of PHA (SCL-SCL) Copolymer by Indigenous Bacillus thuringiensis A102 Strain for Biomedical Applications

Suguna, P.; Saranya, V.; Abirami, P.; Shenbagarathai, R.

doi:10.1007/s00284-020-02221-x

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…This variation of Tm depends on the proportion of 3-hydroxyvalerate (3HV) units, which shape the biopolymer of PHA, in the same way; it affects the resistance and flexibility properties of the polymer [46]. The thermal degradation temperature (Td) for the extracted PHA produced by the SP7-1 strain was in the range of 270 -303 ºC, which was slightly higher than the thermal degradation temperature of the standard PHA (234 -302 ºC), in addition, it was higher than what was reported by Lorini and coauthors [26] who found temperatures in the range of 248 -258 ºC for a PHA extracted from Pseudomonas putida LS46123 and from Suguna and coauthors [45] that reached temperatures up to 269.36 ºC for a PHA from Bacillus thuringiensis. Its thermal stability of the extracted polymer is also related to a more crystalline morphology in its chemical structure [47].…”

Section: Characterization Of Extracted Biopolymermentioning

confidence: 60%

“…Likewise, in the standard PHA a melting temperature of 174.63 °C was observed. The Tm of the PHA of SP7-1 is lower than standard PHA; however, values of Tm around 168 ºC produced by Bacillus megaterium [44] and 138.24 ºC by Bacillus thuringiensis [45] have been reported. This variation of Tm depends on the proportion of 3-hydroxyvalerate (3HV) units, which shape the biopolymer of PHA, in the same way; it affects the resistance and flexibility properties of the polymer [46].…”

Section: Characterization Of Extracted Biopolymermentioning

confidence: 89%

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Production of Polyhydroxyalkanoate by Bacillus thuringiensis Isolated from Agricultural Soils of Cascas-Peru

Cueva-Almendras

Alvarado

Fuentes-Olivera

et al. 2022

Braz. arch. biol. technol.

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Polyhydroxyalkanoates (PHA) are biodegradable biopolymers of microbial origin that can be alternative materials to decrease the extensive use of plastics of petrochemical or synthetic origin. Thus, the selection of microorganisms with potential for PHA production from unexplored natural sources is a strategy to find bacterial species of high value. In the current study, 55 microbial strains related to bacteria were isolated from agricultural soils from Cascas, Peru. Initially, 4 strains were selected by Sudan Black B staining and Nile blue A fluorescence methods, subsequently, they were screened to examine its production capacity of PHA. Bacillus thuringiensis SP7-1 strain was selected based on its high production of PHA at 0.54 ± 0.16 g/L with an accumulation of 19 % by weight of cell biomass, during 72 h at 30 ºC. The isolate was characterized by its morphology, biochemical and molecular tests through the 16S rRNA gene. The extracted HIGHLIGHTS• Bacillus thuringiensis SP7-1 from agricultural soils producing polyhydroxyalkanoate.• The strains SP7-1 with a PHA accumulation of 0.54 g/L and 19 % in dry biomass.• PHA was characterized with FTIR, DSC and TGA with remarkable chemical properties.• A thermic degradation in a range of 270-303°C and a Melting Temperature of 166.88°C.

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Section: Characterization Of Extracted Biopolymermentioning

confidence: 60%

Section: Characterization Of Extracted Biopolymermentioning

confidence: 89%

Production of Polyhydroxyalkanoate by Bacillus thuringiensis Isolated from Agricultural Soils of Cascas-Peru

Cueva-Almendras

Alvarado

Fuentes-Olivera

et al. 2022

Braz. arch. biol. technol.

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“…Specifically, a peak was detected at 2933 cm −1 corresponding to the CH methylene vibration. This strong vibration indicates the existence of a PHB‐co‐PHV co‐polymer within a range of 2933 to 2975 cm −1 33 …”

Section: Resultsmentioning

confidence: 94%

“…This strong vibration indicates the existence of a PHBco-PHV co-polymer within a range of 2933 to 2975 cm −1 . 33…”

Section: Characterization Of the Polymer By Ftirmentioning

confidence: 99%

Utilization of palm oil and palm oil effluent as a carbon source for the production of polyhydroxybutyrate co‐polyhydroxyhexanoate P(HB‐co‐HHx) a biopolymer in bacillus sp

Katagi,

Arekal,

Divyashree

2024

J of Chemical Tech & Biotech

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BACKGROUNDBiodegradable polymers are gaining a reputation as demand is increasing globally. Amongst these Polyhydroxyalkanoates (PHA) are attracting market attention as a consequence of their properties similar to those of conventional plastics. PHA is a natural polyester that is stored as an intracellular carbon (C) energy reserve of bacteria. This biopolymer can be extracted and used in place of petrochemical plastic. Medium chain‐length (mcl) PHA, specifically poly hydroxybutyrate‐co‐hydroxyhexanoate P (HB‐co‐HHx) copolymer produced by Bacillus, has gained practical attention owing to its superior physicochemical properties compared to the commonly synthesized PHB homopolymer. Co‐polymer production is dependent on C sources provided in the growth medium. In the present study we have employed palm oil and palm oil effluent as C source.RESULTSIn the current study, it was observed that a significant amount of PHA and biomass was produced when the effluent was prepared at a concentration of 100% instead of water for preparing the growth medium. The production of biomass and PHA showed a range of 4.8–8and 1.5–4 g L−1, respectively. This means that the PHA yield obtained using the effluent was 50–80% of the control, whereas the control yielded only 40–50%.CONCLUSIONPalm industry waste can be used as a substrate in bioprocessing industries. Proton nuclear magnetic resonance analysis of PHA obtained herein indicates that it consists of the mcl polymer P (HB‐co‐HHx). © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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Biotechnological advances in Bacillus thuringiensis and its toxins: Recent updates

Azizoğlu

Jouzani

Sansinenea

et al. 2023

Rev Environ Sci Biotechnol

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Optimization and Characterization of PHA (SCL-SCL) Copolymer by Indigenous Bacillus thuringiensis A102 Strain for Biomedical Applications

Cited by 8 publications

References 42 publications

Production of Polyhydroxyalkanoate by Bacillus thuringiensis Isolated from Agricultural Soils of Cascas-Peru

Production of Polyhydroxyalkanoate by Bacillus thuringiensis Isolated from Agricultural Soils of Cascas-Peru

Utilization of palm oil and palm oil effluent as a carbon source for the production of polyhydroxybutyrate co‐polyhydroxyhexanoate P(HB‐co‐HHx) a biopolymer in bacillus sp

Biotechnological advances in Bacillus thuringiensis and its toxins: Recent updates

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