Optimization and Chemical Characterization of Biosurfactant Produced from a Novel Pseudomonas guguanensis Strain Iraqi ZG.K.M

Faisal, Zeena G.; Mahdi, Mayaada S.; Alobaidi, Khalid H.

doi:10.1155/2023/1571991

Cited by 13 publications

(18 citation statements)

References 31 publications

(104 reference statements)

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“…These findings bear conformity with those obtained by Piegza et al (2021), where they reported the main components of fatty acids, including 3-hydroxy-stearic acid (C18:0), 3-hydroxypalmitic acid (C16:0), acid octadecene (C18:1, double bond in position 2) represent the biosurfactant moiety in Trichoderma citrinoviride HL and C1 supernatants. Also, fatty acids; n-hexadecanoic acid, methyl linoleate, ethyl palmitate, 8-octadecenoic acid, methyl stearate, methyl ester, ethyl stearate, dodecanoic acid, methyl palmitate, linoleic acid, 9,17-octadecadienal and 2,4-ditert-butylphenol were showed in culture filtrate of Pseudomonas guguanensis Strain Iraqi ZG.K.M by Ghazi et al (2023).…”

Section: Discussionmentioning

confidence: 93%

Biosurfactants production by some Aspergilli species under solid state fermentation

El-Halmouch,

Nasr,

Al-Sodany

et al. 2024

Egypt. J. Bot.

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B IOSURFACTANTS are active secondary metabolites producing` by certain microorganisms that have the ability to reduce surface and interfacial tension. They have numerous advantages, such as high biodegradability, increased foaming, low toxicity and performance under challenging conditions. Current study was compared between the potential production of biosurfactant by A. carneus OQ152507, A. niger OQ195934 and A. fumigatus OQ195264 using solid state fermentation (SSF) as a low-cost alternative technology. These isolates were cultivated under SSF using different eight agricultural wastes to produce biosurfactants. The optimized bioprocess conditions for the maximum biosurfactant productivity were potato peels waste (20g/l) as a carbon source at 35ºC and pH 7 in glucose peptone medium for A. carneus OQ152507, sun flower seed shells (28g/l) at 35ºC and pH 7 in starch nitrate medium for A. niger OQ195934 and tea wastes (20g/l) at 30ºC and pH 6 in glucose peptone medium for A. fumigatus OQ195264. Their stability was observed at 120°C, 30g/l of NaCl and alkaline pH 10 for A. carneus and pH9 for A. niger and A. fumigatus biosurfactants. The biosurfactants were identified as glycolipid and phospholipids molecules by Phenol-H 2 SO 4 and phosphate test. GC-MS and FT-IR analysis confirmed that the tested fungi participate in producing three hydrophobic fatty acid chains: Cis-Vaccenic acid, n-Hexadecanoic acid and Hexadecanoic acid 2-hydroxy-1 (hydroxymethyl) ethyl ester in distinct peaks with the highest percentage area. While, each one has at least one specific biosurfactant moiety.

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Section: Discussionmentioning

confidence: 93%

Biosurfactants production by some Aspergilli species under solid state fermentation

El-Halmouch,

Nasr,

Al-Sodany

et al. 2024

Egypt. J. Bot.

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“…The color of biosurfactants generally varies according to the producing strain, the culture media composition and the extraction technique. The colors reported in the literature were either whitish, yellowish or brownish [64]. Fig.…”

Section: Color Of Biosurfactantsmentioning

confidence: 93%

“…Methylene blue is sometimes added in the culture media to ease the observation of the precipitation line materialized as a blue halo around well containing culture [52]. This method was successfully used to confirm the anionic nature of biosurfactants derived from Lactococcus lactis [63], E. faecium [51], and Pseudomonas guguanensis strain Iraqi ZG.K.M [64]. However, the method is not suitable for other types of biosurfactants such as lipopeptides or glycoproteins.…”

Section: Identification Of Ionic Properties Of Biosurfactantsmentioning

confidence: 99%

“…The presence of lipids in biosurfactants is generally assessed on TLC plates. The principle is based on the reaction between iodine vapors and lipids in a close chamber that leads to the development of a yellow color [64,66]. The method is not specific as a positive result might indicate the presence of neutral or polar lipids [65].…”

Section: Screening Of Lipidsmentioning

confidence: 99%

“…The fatty acids obtained are transformed into a volatile derivate (fatty acid methyl esters) which will be easily analyzed in GC-FID using standards or in GC-MS. Fatty acid methyl esters are separated according to their chain length, and their molecular mass registered provide information on their structures. The fatty acids profile of biosurfactants were determined by several authors in the literature using GC-MS [17,42,51,54,64,71,73,85].…”

Section: Fatty Acids Profile Of Biosurfactantsmentioning

confidence: 99%

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Methods of Purification and Characterization of Biosurfactants: An Overview

Mouafo

Pahane

Arsène

et al. 2023

JABB

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Biosurfactants are amphiphilic molecules produced by several microorganisms including bacteria, filamentous fungi and yeasts. They are classified according to their chemical composition (glycolipids, lipopeptides, glycoproteins, glycolipopeptides, phospholipids) and their molecular weight (low and high molecular weight). Biosurfactants are currently used in several industrial fields (food industry, pharmacy, medicine, detergents, agriculture, cosmetics, oil recovery and bioremediation) owning to some of their specific properties such as their low toxicity, biocompatibility, biodegradability, environmentally-friendly, stability under extreme conditions (temperature, pH and salinity), structural diversity, production using renewable low-cost substrates, agreement with green chemistry and sustainability. Despite the properties of biosurfactants, there are few studies leading to their structural characterization. The most reported structures are from biosurfactants produced by Bacillus spp. (surfactins, fengycins, lichenysines) and Pseudomonas spp. (rhamnolipids). The majority of researches performed on biosurfactants emphasized their functional groups characterization. These biosurfactants have yet to be fully characterized at structural level. Hence, in this review, we highlight the different purification and structural characterization techniques which can be combined to provide information on the structure of the biosurfactants independently of its complexity. This will lead to enhance their application in some highly pointed industrial fields such as medicine and pharmacy.

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Upcycling of Beer Industry Wastewater: Biosurfactant Production and Application in Brewery Effluent Treatment

Sant'Ana,

Latorres,

Guimarães Martins

2024

Environmental Quality Mgmt

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The beer processing industry generates large amounts of effluents rich in organic and inorganic material, and complex and expensive systems are commonly used for their treatment. Therefore, new alternatives capable of ensuring sustainable industrial growth through cost‐effective solutions are being studied. In this context, biosurfactants stand out due to their ability to degrade and remove contaminants from the environment. Therefore, the objective of this study was to evaluate the potential treatment of effluents from the beer industry using biosurfactants produced from wastewater from the beer industry. The biosurfactants were obtained by submerged cultivation at 30°C for 48 h using the bacterium Corynebacterium aquaticum in a mineral medium added of carbon source. The characterization of the biosurfactants was performed in relation to surface tension, emulsifying activity (EA), pH, and ionic character. The analyses were performed at 0, 24, and 48 h. The efficiency of the effluent treatments was evaluated by characterization in duplicate, before, during and after the treatment, regarding the concentrations of biochemical oxygen demand (BOD), chemical oxygen demand (COD), and total solids (TS). The best biosurfactant results were obtained using C. aquaticum in a medium with 10% (v/v) of brewing industry wastewater (BIW) as a carbon source after 48 h of cultivation, reaching a surface tension of 35.1 ± 0.2 mN/m, EA of 40.9 ± 0.5%, and stability under extreme conditions of temperature (−20°C to 121°C), salt (1% to 10%), and pH (4 to 10). Thus, the biosurfactant was applied for 10 days in an aerated biological treatment system. Best results were observed in biosurfactant concentrations of 0.5 CMD (critical micellar dilution), reaching improvement of up to 55.9% for BOD, 23.8% for COD, and 50.6% for TS. Thus, the biosurfactant produced (10% BIW) shows potential as an accelerator in the brewing industry effluent (BIE) treatment process, without purification and sterilization of the biocompound. Therefore, brewery wastewater proved to be a sustainable and nutritional substrate to obtain biosurfactants.

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Optimization and Chemical Characterization of Biosurfactant Produced from a Novel Pseudomonas guguanensis Strain Iraqi ZG.K.M

Cited by 13 publications

References 31 publications

Biosurfactants production by some Aspergilli species under solid state fermentation

Biosurfactants production by some Aspergilli species under solid state fermentation

Methods of Purification and Characterization of Biosurfactants: An Overview

Upcycling of Beer Industry Wastewater: Biosurfactant Production and Application in Brewery Effluent Treatment

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