Optimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assay

Abstract

H5pp provide a reliable and safe alternative for sero-diagnosis and sero-epidemiology of H5N1 infections in a BSL-2 setting.

Kinetics of serological responses in influenza A(H7N9)-infected patients correlate with clinical outcome in China, 2013

“…We explored the kinetics of the serological responses to this novel virus in haemagglutination inhibition (HI) assays and in a recently developed H7N9 pseudotype virus particle neutralisation (Nab) test [10]. Viral pseudotypes have been previously shown to provide reliable correlation with conventional microneutralisation tests for influenza A(H5N1) serological studies [11]. We investigated correlations between serological responses and clinical outcome.…”

Seroprevalence of Pandemic A(H1N1) pmd09 Virus Antibodies in Mexican Health Care Workers Before and After Vaccination

“…Thus, there are many studies that support the use of retroviral pseudotypes bearing hemagglutinin (HA) as a substitute for influenza viruses in neutralization assays whose application allows the work in a Biosafety Level 2 environment. In such studies, it has been demonstrated that neutralization of retroviral pseudotypes is as sensitive and specific as the HI and MN using live virus, proving to be a valid alternative for the detection and quantification of neutralizing antibodies against different influenza virus subtypes (6,27,29,30). In the same way, Temperton and colleagues demonstrated that the results of assays using retroviral pseudotypes bearing HA for determining H5N1 neutralizing antibodies in sera from humans that recovered from infection and sera from animals experimentally immunized correlated with those from HI and MN (29).…”

Evaluation of serological tests for H5N1 avian influenza on field samples from domestic poultry populations in Vietnam: Consequences for surveillance

“…Beta prior 216 distributions were defined using informative prior information for the Se and Sp of the H5pp 217 VNT test (based on Garcia and al, 2010) and the prevalence of the 2 populations (unpublished 218 data from author Desvaux) (see Table 1 for details). Non informative priors were used for the 219 Se and Sp of HI test and the correlation between tests (beta distributions (1,1) equivalent to 220 uniform distributions (0,1)).…”