Optimization and fed-batch production of PHB utilizing dairy waste and sea water as nutrient sources by Bacillus megaterium SRKP-3

Pandian, S; Deepak, V.; Kalishwaralal, Kalimuthu; Rameshkumar, Neelamegam; Jeyaraj, M.; Gurunathan, Sangiliyandi

doi:10.1016/j.biortech.2009.08.040

Cited by 162 publications

(27 citation statements)

References 33 publications

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“…The production PHB by B. casei MSI04 was optimum at pH 7.5. But higher pH of 8.0 and 9.0 respectively were reported as optimum for PHB production [20,21]. The isolate MSI04 was halo-tolerant, it can grow well up to 12% NaCl and the production maximum was attained with 3% NaCl.…”

Section: Discussionmentioning

confidence: 99%

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

et al. 2014

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BackgroundVibrio pathogens are causative agents of mid-culture outbreaks, and early mortality syndrome and secondary aetiology of most dreadful viral outbreaks in shrimp aquaculture. Among the pathogenic vibrios group, Vibrio alginolyticus and V. harveyi are considered as the most significant ones in the grow-out ponds of giant black tiger shrimp Penaeus monodon in India. Use of antibiotics was banned in many countries due to the emergence of antibiotic-resistant strains and accumulation of residual antibiotics in harvested shrimp. There is an urgent need to consider the use of alternative antibiotics for the control of vibriosis in shrimp aquaculture. Biofilm formation is a pathogenic and/or establishment mechanism of Vibrio spp. This study aims to develop novel safe antibiofilm and/or antiadhesive process using PHB to contain vibrios outbreaks in shrimp aquaculture.ResultsIn this study a poly-hydroxy butyrate (PHB) polymer producing bacterium Brevibacterium casei MSI04 was isolated from a marine sponge Dendrilla nigra and production of PHB was optimized under submerged-fermentation (SmF) conditions. The effect of carbon, nitrogen and mineral sources on PHB production and enhanced production of PHB by response surface methods were demonstrated. The maximum PHB accumulation obtained was 6.74 g/L in the optimized media containing 25 g/L starch as carbon source, 96 h of incubation, 35°C and 3% NaCl. The highest antiadhesive activity upto 96% was recorded against V. vulnificus, and V. fischeri, followed by 92% against V. parahaemolyticus and V. alginolyticus and 88% inhibition was recorded against V. harveyi.ConclusionIn this study, a thermostable biopolymer was chemically characterized as PHB based on 1HNMR spectra, FT-IR and GC-MS spectra. The NMR spectra revealed that the polymer was an isocratic homopolymer and it also confirmed that the compound was PHB. The antiadhesive activity of PHB was determined in microtitre plate assay and an effective concentration (EC) of PHB (200 μl containing 0.6 mg PHB) was confirmed by confocal laser scanning microscopic analysis of vibrio biofilm on pre-treated glass and polystyrene surfaces. This is a first report on anti-adhesive activity of PHB against prominent vibrio pathogens in shrimp aquaculture.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-014-0114-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

et al. 2014

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“…Pulse feeding of substrate, i.e., addition of small known amounts of substrate when the substrate is at low concentration in the bioreactor, has been a widely used fed-batch strategy for P(3HB) production. [59][60][61][62][63] This could be mainly attributed to the ease of operation of this strategy, which essentially involves measurement of residual substrate in the bioreactor at several periods during cultivation, and subsequent addition of concentrated substrate to the bioreactor when it falls below a limiting value. This addition helps to avoid limitation of substrate in the fermentation medium, thereby allowing continued microbial growth and product formation.…”

Section: Fed-batch Fermentationmentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible intracellular polyesters that are accumulated as energy and carbon reserves by bacterial species, under nutrient limiting conditions. Successful large-scale production of PHAs is dependent on three crucial factors, which include the cost of substrate, downstream processing cost, and process development. In this respect, design and implementation of bioprocess strategies for efficient PHA bioconversions enable high PHA concentrations, yields and productivities. Additionally, development of PHA fermentation processes using inexpensive substrates, such as agro-industrial wastes, facilitates further cost reduction, thus benefitting large-scale PHA production. Thus, the aim of this review is to highlight various bioprocess strategies for high production of PHAs and their novel copolymers in relatively large quantities. This review also discusses the application of kinetic analysis and mathematical modelling as important tools for process optimization and thus improvement of the overall process economics for large-scale production of PHAs.

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“…This implausible behavioural property can be considered towards industrial production aspects and issues related to removal of pollutants like PAHs, pesticides, dyestuffs, and so forth. In concern to increasing load of industrial waste on soil, researchers are trying all possible alternatives to use different wastes like textile effluent [4,8], distillery effluent [9], dairy wastes [10][11][12], oil mill wastes [13], starchy waste [14,15], paper mill wastes [16], animal residues [17], biodiesel waste by-product [18], and agricultural wastes [19], for PHA production. PHAs are produced under nutrient limiting conditions with excess of carbon [20] and get stored in polymerized form as inclusions bodies [21].…”

Section: Introductionmentioning

confidence: 99%

Biotechnological Production of Polyhydroxyalkanoates: A Review on Trends and Latest Developments

Saharan

Grewal

Kumar

2014

Chinese Journal of Biology

113

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Polyhydroxyalkanoates (PHA) producers have been reported to reside at various ecological niches which are naturally or accidently exposed to high organic matter or growth limited conditions such as dairy wastes, hydrocarbon contaminated sites, pulp and paper mill wastes, agricultural wastes, activated sludges of treatment plants, rhizosphere, and industrial effluents. Few among them also produce extracellular by-products like rhamnolipids, extracellular polymeric substances, and biohydrogen gas. These sorts of microbes are industrially important candidates for the reason that they can use waste materials of different origin as substrate with simultaneous production of valuable bioproducts including PHA. Implementation of integrated system to separate their by-products (intracellular and extracellular) can be economical in regard to production. In this review, we have discussed various microorganisms dwelling at different environmental conditions which stimulate them to accumulate carbon as polyhydroxyalkanoates granules and factors influencing its production and composition. A brief aspect on metabolites which are produced concomitantly with PHA has also been discussed. In conclusion, exploring of capabilities like of dual production by microbes and use of wastes as renewable substrate under optimized cultural conditions either in batch or continuous process can cause deduction in present cost of bioplastic production from stored PHA granules.

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Optimization and fed-batch production of PHB utilizing dairy waste and sea water as nutrient sources by Bacillus megaterium SRKP-3

Cited by 162 publications

References 33 publications

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic vibrios

Strategies for Large-scale Production of Polyhydroxyalkanoates

Biotechnological Production of Polyhydroxyalkanoates: A Review on Trends and Latest Developments

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