Optimization And Kinetic Analysis On The Production Of Hyaluronic Acid By Streptococcus Zooepidemicus In A Batch System

Abstract

In this study, the optimization and kinetic analysis on the effect of process parameters for the production of hyaluronic acid production by S. zooepidemicus were performed in batch system. The fermentation condition such as inoculum size (2 to 20 %), pH (4 to 10), temperature (30 to 45) and agitation (50 to 500) were optimized using “one-factor-at-a-time” method. It was found that the inoculum size of 10 % had the greatest effect on the fermentation process which gave the highest value of specific growth rate, cell biomass and HA production of 0.72 h−1, 1.96 gl−1 and 0.82 gl−1, respectively. Furthermore, the other process parameters such as pH, temperature and agitation speed were also found to improve the cell biomass, HA production and kinetic analysis. The highest cell biomass (0.17 gcell gglucose−1) and HA yield (0.096 gHA g glucose−1) were obtained at pH 7.0, 37 °C and agitation speed 300 rpm for 12 h. The overall kinetics analysis of maximum specific growth rate, volumetric cell biomass productivity and volumetric HA productivity obtained at optimum parameters were, 0.81 h−1, 0.183 gcell l−1 h−1 and 0.111 gHA l−1 h−1, respectively.

Hyaluronic Acid: A Powerful Biomolecule with Wide-Ranging Applications—A Comprehensive Review

“…In addition to the pH, the incubation temperature, agitation speed, soil composition, and size of inoculum are key parameters of the fermentation process that affect the yield of the final product. Some researchers found that a streptococcal inoculum size of 10% had the greatest effect on the fermentation process and also that an agitation speed of 300 rpm could increase the HA yield [ 90 ]. This may have occurred because at this rate of speed, a greater subdivision of air bubbles could occur with a consequent larger surface area for the gas–liquid mass transfer, which therefore led to a reduction in the thickness of the gas and liquid films responsible for the resistance to the mass transport [ 90 ].…”

“…Some researchers found that a streptococcal inoculum size of 10% had the greatest effect on the fermentation process and also that an agitation speed of 300 rpm could increase the HA yield [ 90 ]. This may have occurred because at this rate of speed, a greater subdivision of air bubbles could occur with a consequent larger surface area for the gas–liquid mass transfer, which therefore led to a reduction in the thickness of the gas and liquid films responsible for the resistance to the mass transport [ 90 ]. In this way, it is easy to understand that several parameters must be optimized in order to enhance the microbial production of HA starting from natural producer microorganisms.…”

Innovative strategies for biotechnological production of hyaluronic acid from different sources

“…The type of microbe used in bio-production will determine the optimal temperature for generating polysaccharides like HA (Liu et al, 2018) and (Shoparwe et al, 2020).5.2.4. pH:One of the most crucial factors that must be managed in the majority of fermentation processes is the medium's pH. It has been demonstrated that pH has a greater impact on polysaccharide formation than on cell proliferation(Shoparwe et al, 2020). HA is particularly sensitive to pH fluctuations, where it was found that HA is broken down by hydrolysis at relatively basic (>11) or acidic (4.0) pH levels.…”