Optimization and Production of Alkaline Proteases from Agro Byproducts Using a Novel <i>Trichoderma viridiae</i> Strain VPG 12, Isolated from Agro Soil

Abstract

In recent years, there has been a phenomenal increase in the use of alkaline proteases as industrial catalysts. The aim of this work was to isolate potent fungal strain from the agricultural field of Gulbarga region of India, for the production of alkaline protease by utilizing the agricultural by products viz, red and green gram and Bengal gram as substrate under submerged fermentation process. Optimization of fermentation process parameters such as substrate (Red gram husk, green gram husk and Bengal gram husk) utilization, utilization, temperature, pH and incubation period for alkaline protease production was carried out. The maximum production of alkaline protease by Trichoderma VPG 12 was found at pH 8, temperature 35 °C, incubated for 120 h. But the activity of the enzyme could also be seen in a wide range of pH (5-9) and temperature (20-40 °C). With all these properties, the strain can be considered for industrial grade production of alkaline protease.

An enhanced fat extraction from Pycnanthus angolensis (African nutmeg) seeds using cellulase from Aspergillus niger strain BC23

“…This situation suggested a feasible harvest (enzyme) at about 96 h (on day 4) of fermentation, similar to the findings published elsewhere (Abubakar and Oloyede, 2013;Costa et al, 2021;Maftukhah and Abdullah, 2018;Okoye et al, 2013). The incubation period, however, would depend on a number of factors, such as the availability/level of nutrients, fermentation environment, nature of fermenting organisms and the physiological conditions influencing the (fermentation) process (Patil and Dayanand, 2006;Shivasharanappa et al, 2014).…”

Agro-waste as a substrate for the production of pullulanase by Penicillium viridicatum under solid-state fermentation

“… 11 reported maximum production of protease (9550 U/g biomass) by Bacillus species in green gram substrate. However, Shivasharanappa et al 12 reported lower production of protease in green gram husk as compared to red gram and Bengal gram husk. A similar finding was reported by Chmimata et al .…”

Optimization of fermenting medium by statistical method for production of alkaline protease by Bacillus licheniformis MZK05M9

“…Extracellular microbial alkaline proteases are commercially important enzymes used extensively in several industries such as in the detergent, food, pharmaceutical, chemical, leather, silk, and waste treatment [1]. A large proportion of commercially important alkaline proteases is derived from the strains of Bacillus [2][3][4].…”