Optimization and production of polyhydroxybutyrate from sludge by Bacillus cereus categorized through FT-IR and NMR analyses

Narayanan, Mathiyazhagan; Kandasamy, Gajendiran; Murali, P.; Kandasamy, Sabariswaran; Ashokkumar, Veeramuthu; Nasif, Omaima; Pugazhendhi, Arivalagan

doi:10.1016/j.jece.2020.104908

Cited by 34 publications

(13 citation statements)

References 43 publications

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“…During this time, referred to as 'feast and famine condition' the organism tends to store a major portion of the substrate as biopolymer as a survival strategy (Moralejo-Gárate et al, 2011;Wang et al, 2017). The substrate storage in the form of polyhydroxyalkanoate is usually thought to serve as a mechanism to overcome adverse growth conditions (Narayanan et al, 2021;Third et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Madhusoodanan

Hariharapura

Divyashree

2021

Environ Dev Sustain

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Conventional biopolymers resembling synthetic polymers produced from microorganisms, polyhydroxyalkanoate (PHA) synthesized utilizing renewable resources have gained supreme attention recently. PHA accumulation within the microbial cell is an innate capability of bacteria to store carbon and energy when nutrient imbalance pertains. Gram positive Bacillus endophyticus capable of synthesizing PHA was focused in this study. Study focuses on the possibility of attaining high PHA yield in relation to the varying dissolved oxygen levels induced during production phase. There was a gradual increment in PHA production from 34.5 to 53.03% when cultivated in bioreactor that maintained least dissolved oxygen of 0.4 mg/L at 32 °C. The metabolic flux of organism was altered during oxygen stress brought by varying agitation rate and volume resulting in the accumulation of Nicotinamide Adenine Dinucleotide Hydrogen (NADH), which led to increase in the overall PHA production. PHA yield was found to be favored by decreasing the oxygen supply thereby inducing an oxygen stress environment. This report was the first one that was correlating the hypothesis that links PHA yield and oxygen stress condition during production phase. PHA produced was characterized by FTIR and 1HNMR spectra in which the presence of Polyhydroxybutyrate was confirmed. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Madhusoodanan

Hariharapura

Divyashree

2021

Environ Dev Sustain

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“…Similarly in this study as well, the significance and dependency of the strain on N, P and Na source were clearly shown as a prompt requirement to increase the cell mass. Later for PHA production, limitation of the nitrogen source was found to be significant which shows the limitation of nutrients except carbon enhances PHA production (Narayanan et al 2021 ). The conclusive finding of the study is that the basal medium can be enriched with essential nutrients for fast cell growth and later during the late log phase reducing the nitrogen source can amplify PHA production.…”

Section: Resultsmentioning

confidence: 99%

Statistical optimisation of polyhydroxyalkanoate production in Bacillus endophyticus using sucrose as sole source of carbon

Geethu

Chandrashekar

2021

Arch Microbiol

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Microorganisms have been contemplated as a promising source for the inexhaustible synthesis of many novel materials utilizing renewable sources. Among many of such products, polyhydroxyalkanoate (PHA) remains as an essential biodegradable polymer with functions similar to conventional plastics. Bacillus endophyticus is capable of accumulating biopolymer PHA in nutrient limiting conditions with excess of carbon source. Screening and optimizing the parameters for increased PHA production was done statistically. The optimized medium gave a maximum yield of 46.57% which was in well agreement with the given predicted value provided by response surface methodology model yield of 47.02%. Optimal media conditions when extrapolated in bioreactor gave an even higher production percentage of 49.9. This is the first report highlighting 49% of polyhydroxybutyrate statistically using sucrose as a source. The main highlight of the study was the use of wild type strain for producing high quality PHA using simple carbon source which can be a starting platform for using this strain for large scale PHA production industrially. FTIR and 1HNMR analysis confirmed the polymer produced.

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“…Similarly in this study as well, the significance and dependency of the strain on N, P and Na source were clearly showed as a prompt requirement to increase the cell mass. Later for PHA production, limitation of the nitrogen source was found to be significant which shows the limitation of nutrients except carbon enhances PHA production (Narayanan et al 2021). The conclusive finding of the study is that the basal medium can be enriched with essential nutrients for fast cell growth and later during the late log phase reducing the nitrogen source can amplify PHA production.…”

Section: Interaction Of Variables On Responsesmentioning

confidence: 99%

“…The peak at 2.57ppm, Doublet of quadruplet at corresponding methylene group adjacent to an asymmetric carbon bearing single atom was observed. The methylene group characteristic Multiplet was found precisely on 5.27 was determined from the 1 H NMR spectra(Narayanan et al 2021).…”

mentioning

confidence: 99%

Statistical Optimisation of Polyhydroxyalkanoate Production in Bacillus Endophyticus Using Sucrose as Sole Source of Carbon

Geethu

Hariharapura²,

Divyashree

2021

Preprint

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Microorganisms have been contemplated as a promising source for the inexhaustible synthesis of many novel materials utilizing renewable sources. Among many of such products, Polyhydroxyalkanoate (PHA) remains as an essential biodegradable polymer with functions similar to conventional plastics. Bacillus endophyticus is capable of accumulating biopolymer PHA in nutrient limiting conditions with excess of carbon source. Screening and optimizing the parameters for increased PHA production was done statistically. The optimized medium gave a maximum yield of 46.57 % which was in well agreement with the given predicted value provided by Response surface Methodology model yield of 47.02 %. Optimal media conditions when extrapolated in bioreactor gave an even higher production percentage of 49.9.This is the first report highlighting 49 % of Polyhydroxybutyrate statistically using sucrose as a source. The main highlight of the study was the use of wild type strain for producing high quality PHA using simple carbon source which can be a starting platform for using this strain for large scale PHA production industrially. FTIR and 1HNMR analysis confirmed the polymer produced.

show abstract

Optimization and production of polyhydroxybutyrate from sludge by Bacillus cereus categorized through FT-IR and NMR analyses

Cited by 34 publications

References 43 publications

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Statistical optimisation of polyhydroxyalkanoate production in Bacillus endophyticus using sucrose as sole source of carbon

Statistical Optimisation of Polyhydroxyalkanoate Production in Bacillus Endophyticus Using Sucrose as Sole Source of Carbon

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