Optimization and validation of a fast RP–HPLC method for the determination of dobutamine in rat plasma: Pharmacokinetic studies in healthy rat subjects

Abstract

A novel isocratic reverse phase high performance liquid chromatography (RP–HPLC) with photo diode array (PDA) detection method for the determination of dobutamine (DBT) in rat plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Homoveratrylamine was used as an internal standard. Methanol was used as the extracting solvent for the preparation of plasma samples. Samples were separated on a Symmetry C18 (250 mm×4.6 mm i.d., 5 μm) analytical column. Acetonitrile and 15 mM potassium dihydrogen phosphate (pH 5.0 with 0.3% TEA) (20:80, v/v) was used. The column oven temperature was optimized at 35 °C and the flow rate was 0.8 mL/min. The detection wavelength was fixed at 230 nm for entire analysis. The calibration curve was found to be linear over the concentration range of 50–2000 ng/mL (r2=0.9992). The limit of quantification (LOQ) of the method was 50 ng/mL. The % RSD values of accuracy and precision values for intra and inter days were <15% at quality control (QC) concentrations. Recovery, stability and robustness were studied within the acceptable range according to ICH guidelines. The method was efficiently applied to a pharmacokinetic study in healthy Wistar rats.

Electrochemical sensing of dobutamine, paracetamol, amlodipine, and daclatasvir in serum based on thiourea SAMs over nano-gold particles–CNTs composite

“…10,11,19,20,78–82 Also, the TU-SAMs sensor exhibits low DLs for DB, PA, AM, and DAC determination compared to other reported methods in the literature, Table S2 (ESI†). 8,9,29,30,35,36,43,44…”

“…10,11,19,20,[78][79][80][81][82] Also, the TU-SAMs sensor exhibits low DLs for DB, PA, AM, and DAC determination compared to other reported methods in the literature, Table S2 (ESI †). 8,9,29,30,35,36,43,44 Thus, the synergistic effect between all the modifiers of the composite; CNTs, Au nano and TU, enhances the kinetic process for electro-oxidation of the studied drugs.…”

“…19 Therefore, it is important to develop a sensitive approach to determine the concentrations of DB in biological fluids. Determination of DB was previously reported by different methods such as reverse phase high-performance liquid chromatography (RP-HPLC), 29 and spectrophotometric methods. 30 AM, a derivative of dihydropyridine, is known as a calciumchannel blocker drug.…”

Pharmacokinetic Herb-Drug Interactions of Glipizide with Andrographis paniculata (Burm. f.) and Andrographolide in Normal and Diabetic Rats by Validated HPLC Method

“…Validation of the percentage differentiation parameter of ≤20% revealed that the developed analytical method acquired a LLOQ of 25 ng/mL, which is comparable with the values in earlier investigations at a concentration of 50 ng/mL [ 46 ]. Therefore, the proposed bioanalytic approach can determine analyte concentrations in plasma with accuracy and precision at the LLOQ [ 47 ].…”

Cassia occidentalis Potentiates wound Healing Process in Type-2 Diabetic Rats

“…MCO was subjected to phytochemical investigation for the detection of the presence of various phytochemicals by using standard methods. [13] Formulation of hydrogel of MCO…”