Optimization and validation of an ischemic wound model

Gould, Lisa; Leong, Mimi; Sonstein, Joseph; Wilson, Shelly

doi:10.1111/j.1524-475x.2005.00080.x

Cited by 49 publications

(56 citation statements)

References 22 publications

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“…Survival of skin flaps is known to depend on their dimension, which in turn determines the graded levels of ischemia throughout the flap tissue (18,27,28). We chose to force skin ischemia by adopting a full-thickness bipedicle flap approach as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The dermal flap was elevated from the underlying subcutaneous tissue. Precut (same as that of the dimensions of the flap), sterilized 0.01-inch-thick Sil-Tec medical-grade sheeting (Technical Products Inc. of Georgia, Decatur, GA) was then placed underneath the flap to prevent readherence and reperfusion of the flap from the underlying tissue (18). The skin flap incisions and silicon sheet were sutured into position using 3-O Ethilon continuous sutures.…”

Section: Porcine Ischemic Excisional Wound Modelmentioning

confidence: 99%

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Characterization of a preclinical model of chronic ischemic wound

Roy

Biswas

Khanna

et al. 2009

Physiological Genomics

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111

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Chronic ischemic wounds presenting at wound clinics are heterogeneous with respect to etiology, age of the wound, and other factors complicating wound healing. In addition, there are ethical challenges associated with collecting repeated biopsies from a patient to develop an understanding of the temporal dynamics of the mechanisms underlying chronic wounds. The need for a preclinical model of ischemic wound is therefore compelling. The porcine model is widely accepted as an excellent preclinical model for human wounds. A full-thickness bipedicle flap approach was adopted to cause skin ischemia. Closure of excisional wounds placed on ischemic tissue was severely impaired resulting in chronic wounds. Histologically, ischemic wounds suffered from impaired re-epithelialization, delayed macrophage recruitment and poorer endothelial cell abundance and organization. Compared with the pair-matched nonischemic wound, unique aspects of the ischemic wound biology were examined on days 3, 7, 14, and 28 by systematic screening of the wound tissue transcriptome using high-density porcine GeneChips. Ischemia markedly potentiated the expression of arginase-1, a cytosolic enzyme that metabolizes the precursor of nitric oxide l-arginine. Ischemia also induced the SOD2 in the wound tissue perhaps as survival response of the challenged tissue. Human chronic wounds also demonstrated elevated expression of SOD2 and arginase-1. This study provides a thorough database that may serve as a valuable reference tool to develop novel hypotheses aiming to elucidate the biology of ischemic chronic wounds in a preclinical setting.

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Section: Resultsmentioning

confidence: 99%

Section: Porcine Ischemic Excisional Wound Modelmentioning

confidence: 99%

Characterization of a preclinical model of chronic ischemic wound

Roy

Biswas

Khanna

et al. 2009

Physiological Genomics

Self Cite

111

View full text Add to dashboard Cite

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“…However, their disadvantages include those of their differences when compared to human i.e. size, metabolic characteristics and anatomy 30 .…”

Section: Post-burn Time Intervalmentioning

confidence: 99%

Rat an experimental model for burns: A systematic review

Mitsunaga

Gragnani

Ramos³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To revise and systematize scientific knowledge of the experimental model for cutaneous burns in rats. METHODS:A bibliographical review from 2008 up to January 2011 in PubMed, EMBASE and LILACS was undertaken. Were used the keywords: animal models, burns and rats. 221 studies were identified, and 116 were selected. RESULTS:It was found that: 54/86 (62.7%) had third degree burns; 55/73 (75.3%) studied the back; 45/78 (57.6%) used heated water and 27/78 (35.9%) incandescent instruments; 39/78 (50%) studied systemic effects; 22/71 (31%) used ketamine associated with xylazine; 61/64 (95.3%) performed depilation with appropriate equipment; 36/72 (50%) used microscopy; more than 50% did not describe analgesia or antibiotics during the postoperative period; in 42/116 (36.2%) postoperative fluid therapy was performed; and the time interval after the burn, up to the beginning of the results analysis varied from 7s up to four weeks. Legislation issues on burn experiments are discussed. CONCLUSION:The hot water was the main method to induce burns those of third degree on the back, with anesthesia using ketamine and xylazine, after depilation. These were evaluated microscopically, without using analgesia or an antibiotic during the postoperative period. The studies were not very reproducible. CONCLUSÃO: Líquido aquecido foi o principal método para induzir queimadura de terceiro grau no dorso do animal, com anestesia usando quetamina e xilazina, após depilação, avaliados por microscopia, sem uso de analgesia ou antibióticos. Os estudos não são reprodutíveis.Descritores: Modelos Animais. Queimaduras. Pele. Revisão. Ratos.Mitsunaga Jr JK et al.

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“…Skin perfusion can be impaired by diseases such as chronic venous insufficiency [2] and diabetes [3], which can lead to decubitus ulcer formations and necrosis. In the wound healing process, vascular supply to the wound is essential and relies on neovascularization or angiogenesis [4], and means of quantitatively characterizing perfusion is crucial for prognosis.…”

Section: Motivationmentioning

confidence: 99%

Evaluation of three inverse problem models to quantify skin microcirculation using diffusion-weighted MRI

Cordier

Choi

Raguin

2008

J. Phys.: Conf. Ser.

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Abstract. Skin microcirculation plays an important role in diseases such as chronic venous insufficiency and diabetes. Magnetic resonance imaging (MRI) can provide quantitative information with a better penetration depth than other noninvasive methods, such as laser Doppler flowmetry or optical coherence tomography. Moreover, successful MRI skin studies have recently been reported. In this article, we investigate three potential inverse models to quantify skin microcirculation using diffusion-weighted MRI (DWI), also known as q-space MRI. The model parameters are estimated based on nonlinear least-squares (NLS). For each of the three models, an optimal DWI sampling scheme is proposed based on D-optimality in order to minimize the size of the confidence region of the NLS estimates and thus the effect of the experimental noise inherent to DWI. The resulting covariance matrices of the NLS estimates are predicted by asymptotic normality and compared to the ones computed by Monte-Carlo simulations. Our numerical results demonstrate the effectiveness of the proposed models and corresponding DWI sampling schemes as compared to conventional approaches.

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Optimization and validation of an ischemic wound model

Cited by 49 publications

References 22 publications

Characterization of a preclinical model of chronic ischemic wound

Characterization of a preclinical model of chronic ischemic wound

Rat an experimental model for burns: A systematic review

Evaluation of three inverse problem models to quantify skin microcirculation using diffusion-weighted MRI

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