Optimization and validation of the direct HPLC method for the determination of moxifloxacin in plasma

Laban-Djurdjević, Aleksandra; Jelikić-Stankov, Milena; Đjurdjević, Predrag

doi:10.1016/j.jchromb.2006.07.001

Cited by 53 publications

(49 citation statements)

References 12 publications

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“…The neutral and zwitterionic forms are expected to get retained by the reverse phase columns. However in aqueous Literature reports present the use of surface response techniques for optimizing the concentration of organic part in the mobile phase 70,71 . The pH of the mobile phase is also an important parameter to be considered and the average pH of analysis was observed 72, 73 between 2.5 to 3.5.…”

Section: Chromatographic Separationmentioning

confidence: 99%

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Untitled

2016

IJPSR

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ABSTRACT:Fluoroquinolones are the most commonly prescribed synthetic antibacterial agents having improved pharmacokinetic properties and broad spectrum of activity. Due to their broad clinical activity they are the most promising drug for antibacterial chemotherapy. The current study focused on the application of similar chromatographic system for the analysis of a number of fluoroquinolones from bulk, pharmaceutical formulations and biological matrices. We have also summarized how a single chromatographic system can be used for the quantification of a number of fluoroquinoloes from their respective marketed formulation. A detailed systemic survey of the sample preparation techniques, the chromatographic conditions and detection of the respective fluoroquinolones has been presented in this study. In the course of this discussion the advantages and applicability of the high performance liquid chromatographic techniques are also examined.

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Section: Chromatographic Separationmentioning

confidence: 99%

“…The use of paired ion chromatography reagents are also reported for fluoroquinolones. Due to their ionizable properties in aqueous media, these reagents are often used to increase their retention 71 . Liang et.…”

Section: Chromatographic Separationmentioning

confidence: 99%

Untitled

2016

IJPSR

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“…In literature, several analytical methods such as RP-HPLC [3][4][5][6][7][8][9][10][11] , UPLC 12 and some hyphenated techniques such as LC/MS/MS 13 , LC-MS 14,15 have been reported for the determination of MOX single and in combination in biological fluids. Few RP-HPLC 16,17 , UV-Spectrophotometric, [18][19][20][21] and HPTLC 22,23 methods have been studied for determination of MOX in bulk and in pharmaceutical formulations.…”

Section: Moxifloxacin Hydrochloride (Mox) Is Chemically 1-cyclopropylmentioning

confidence: 99%

Simultaneous Estimation of Moxifloxacin Hydrochloride and Dexamethasone Sodium Phosphate in Bulk and in Ophthalmic Solution by Rp- HPLC

Dhumal¹,

Shirkhedkar²,

Nerkar³

et al. 2012

J. Chil. Chem. Soc.

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A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous estimation of Moxifloxacin Hydrochloride (MOX) and Dexamethasone Sodium Phosphate (DSP) in Ophthalmic Solution. The method was carried out on a Qualisil RP C-8 (250 mm x 4.6 mm, 5 μm) column with a mobile phase consisting of Methanol: Water (75:25 v/v) pH adjusted to 3.0 with ortho-phosphoric acid of aqueous phase and flow rate of 1.0 mL min -1 . Detection was carried out at 240 nm. The retention time for MOX and DSP was found to be 2.22, and 7.26 min, respectively. The MOX and DSP followed linearity in the concentration range of 10 -60 µg mL -1 and 2-12 µg mL -1 with r 2 = 0.99, respectively. The amounts of both these drugs estimated by proposed method were found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The LOD and LOQ were found to be 0.30 µg mL -1 and 0.91 µg mL -1 for MOX and 0.10 µg mL -1 and 0.30 µg mL -1 for DSP. The proposed method can be used for routine analysis of both these drugs simultaneously in their combined dosage form.

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“…In literature, it is revealed that MOX has been determined in biological fluids: high-performance liquid chromatography (HPLC) [3][4][5][6][7][8][9][10][11], voltammetry [12], and capillary electrophoresis with laser-induced fluorescence [13]. None of them is for breast milk analysis.…”

Section: Introductionmentioning

confidence: 99%

An HPLC method for the determination of moxifloxacin in breast milk by fluorimetric detection with precolumn derivatization

Tekkeli¹,

Gazioğlu²,

Kızıltaş³

2017

Acta Chromatographica

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Summary.A new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile-10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min −1 . The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1-40 µg mL −1 . Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 µg mL −1 , respectively. Intra-day and interday repeatabilities were less than 3.15%.

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Optimization and validation of the direct HPLC method for the determination of moxifloxacin in plasma

Cited by 53 publications

References 12 publications

Untitled

Untitled

Simultaneous Estimation of Moxifloxacin Hydrochloride and Dexamethasone Sodium Phosphate in Bulk and in Ophthalmic Solution by Rp- HPLC

An HPLC method for the determination of moxifloxacin in breast milk by fluorimetric detection with precolumn derivatization

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