Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1

Mathivanan, Krishnamurthy; Uthaya, Chandirika Jayaraman; Mathimani, Thangavel; Vinothkanna, Annadurai; Rajendran, R.; Annadurai, G.

doi:10.1016/j.carbpol.2019.115369

Cited by 65 publications

(24 citation statements)

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“…4A. This result accords with various reports showed that higher EPS production was obtained at neutral pH [13,16,39,40]. While the pH at 6.24 was optimum for EPS production from Agrobacterium radiobacter [36].…”

Section: Optimal Parameters For Eps Production By Agrobacterium Strains (Cs5 and T1)supporting

confidence: 91%

“…However, Shih et al [35] recommended the sucrose concentration of 14% was needed for maximum curdlan production by Agrobacterium sp. Recently, Krishnamurthy et al [13] proposed that sucrose concentration of 5% stimulated maximum EPS production (3.31 g/l) by Bacillus cereus KMS3-1. In the present study, sucrose concentrations beyond 2% for the strain T1 and 3% for the Cs5 led to a decrease in the EPS production.…”

Section: Optimal Parameters For Eps Production By Agrobacterium Strains (Cs5 and T1)mentioning

confidence: 99%

“…Generally, the availability of carbon was accompanied by the limitation of nitrogen which is commonly reported as the better conditions for EPS production by different microbes [18]. Yeast extract was suggested before as an organic nitrogen source to encourage the productivity of the EPS [13,16,21]. Whereas, other researchers recommended different sources of inorganic nitrogen such as urea [35], ammonium phosphate [37], ammonium sulphate [38] and ammonium nitrate [39] to support the production of higher amounts of EPS.…”

Section: Optimal Parameters For Eps Production By Agrobacterium Strains (Cs5 and T1)mentioning

confidence: 99%

“…EPSapplications are more extended to food industries (e.g. stabilizers, thickeners, gelling agents, bioadhesives, probiotic, and emulsifier), cosmetics, textile, oil recovery, metal mining and metal recovery [9][10][11][12][13]. As well, the EPS-producing microbes found in the rhizosphere could be an essential agent for maintaining soil productivity through the improving of the soil structure via aggregation of soil components [14].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial and antioxidant characteristics of exopolysaccharides produced by Agrobacterium tumefaciens Cs5 and T1

et al. 2020

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This work was conducted to isolate and screen for bacteria that producing higher quantities of exopolysaccharides (EPSs) from the Egyptian soils. Forty soil samples were collected from different regions of three Governorates (Beheira, Giza, and Qalyubia). A total of 165 bacterial isolates were obtained from the soil rhizosphere. Thirty-five of them (21.2%) had ability to produce stable ropy colonies. After screening, two isolates Cs5 and T1 were selected for further study according to their capacity to produce a high quantity of EPS (7.1 and 5.7 g/l, respectively). The selected bacterial isolates were identified as non-similar strains of Agrobacterium tumefaciens based on their morphological, biochemical characteristics as well as 16S rRNA gene sequencing. To obtain maximum EPS production of both strains, nutritional and cultivation parameters were optimized using the one-factor-at-a-time method. Thus, the maximum EPS yield of 21.63 and 19.57 g/l were achieved from the strains Cs5 and T1, respectively. When molasses and corn steep liquor were substituted the synthetic carbon and nitrogen sources as low cost-substrates, EPS yields were improved to be 28.73 and 24.5 g/l for Cs5 and T1, respectively. The infrared (FT-IR) spectra for both extracts revealed the typical patterns of polysaccharide absorption. Moreover, HPLC analysis demonstrated that EPSs were heteropolysaccharide composed of glucosamine, glucose, and fructose. The produced EPS showed antioxidant capacity as well as strong antibacterial activity against Staphylococcus aureus (methicillin-resistant) and Bacillus cereus as hazardous human pathogens, suggesting their application in the food industry. While the produced EPS did not affect cancer cells.

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Section: Optimal Parameters For Eps Production By Agrobacterium Strains (Cs5 and T1)supporting

confidence: 91%

Section: Optimal Parameters For Eps Production By Agrobacterium Strains (Cs5 and T1)mentioning

confidence: 99%

Section: Optimal Parameters For Eps Production By Agrobacterium Strains (Cs5 and T1)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antimicrobial and antioxidant characteristics of exopolysaccharides produced by Agrobacterium tumefaciens Cs5 and T1

et al. 2020

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“…During the last decade, plenty of studies were devoted to the isolation and characterization of extremophile bacterial strains and their EPSs, or analyzing their biosorption properties and their bioremediation potential ( Krishnamurthy et al, 2020 ; Joulak et al, 2020a ). However, most of the studies have in common that they were characterized for bioremediation at a lab scale.…”

Section: Application Of Extremophilic Eps For Environment Bioremediationmentioning

confidence: 99%

Extremophilic Exopolysaccharides: Biotechnologies and Wastewater Remediation

et al. 2021

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Various microorganisms thrive under extreme environments, like hot springs, hydrothermal vents, deep marine ecosystems, hyperacid lakes, acid mine drainage, high UV exposure, and more. To survive against the deleterious effect of these extreme circumstances, they form a network of biofilm where exopolysaccharides (EPSs) comprise a substantial part. The EPSs are often polyanionic due to different functional groups in their structural backbone, including uronic acids, sulfated units, and phosphate groups. Altogether, these chemical groups provide EPSs with a negative charge allowing them to (a) act as ligands toward dissolved cations as well as trace, and toxic metals; (b) be tolerant to the presence of salts, surfactants, and alpha-hydroxyl acids; and (c) interface the solubilization of hydrocarbons. Owing to their unique structural and functional characteristics, EPSs are anticipated to be utilized industrially to remediation of metals, crude oil, and hydrocarbons from contaminated wastewaters, mines, and oil spills. The biotechnological advantages of extremophilic EPSs are more diverse than traditional biopolymers. The present review aims at discussing the mechanisms and strategies for using EPSs from extremophiles in industries and environment bioremediation. Additionally, the potential of EPSs as fascinating biomaterials to mediate biogenic nanoparticles synthesis and treat multicomponent water contaminants is discussed.

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Natural Products Produced by the Species of Bacillus cereus Group: Recent Updates

Azizoglu,

Argentel‐Martínez,

Peñuelas‐Rubio

et al. 2024

Journal of Basic Microbiology

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Bacillus cereus group produces diverse antimicrobial compounds through different metabolic pathways, including amino acid‐based compounds, sugar derivatives, volatile and miscellaneous compounds. These antimicrobial compounds exhibit antibacterial and antifungal activities against various plant pathogens, promoting plant growth and enhancing tolerance to abiotic stresses. They also exhibit nematicidal activities against plant nematodes and antagonistic effects against pathogens in aquatic animals, promoting growth and inducing immune responses. Moreover, B. cereus group bacteria play a significant role in bioremediation by breaking down or neutralizing environmental pollutants, such as plastics, petroleum products, heavy metals, and insecticides. They produce enzymes like laccases, lipases, proteases, and various oxidases, contributing to the degradation of these pollutants. In the food industry, they can cause food poisoning due to their production of enterotoxins. However, they are also utilized in various industrial applications, such as producing environmentally friendly bio‐based materials, biofertilizers, and nanoparticles. Notably, B. cereus transforms selenite into selenium nanoparticles, which have health benefits, including cancer prevention. In summary, B. cereus group bacteria have diverse applications in agriculture, bioremediation, industry, and medicine, contributing to sustainable and eco‐friendly solutions across multiple fields. In this review, we have revised B. cereus group and the characteristics of every species; we have also highlighted the more important compounds secreted by the species of B. cereus group and the applications of these compounds. The aim is to explain the available secondary metabolites to classify the species from this group, increasing the knowledge about taxonomy of this group.

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Optimization, compositional analysis, and characterization of exopolysaccharides produced by multi-metal resistant Bacillus cereus KMS3-1

Cited by 65 publications

References 50 publications

Antimicrobial and antioxidant characteristics of exopolysaccharides produced by Agrobacterium tumefaciens Cs5 and T1

Antimicrobial and antioxidant characteristics of exopolysaccharides produced by Agrobacterium tumefaciens Cs5 and T1

Extremophilic Exopolysaccharides: Biotechnologies and Wastewater Remediation

Natural Products Produced by the Species of Bacillus cereus Group: Recent Updates

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