Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro

Improved simultaneous production of mycelial biomass and polysaccharides by submerged culture of Hericium erinaceum: optimization using a central composite rotatable design (CCRD)

“…In comparison to organic nitrogen sources, the mycelial growth on media containing inorganic nitrogen sources was comparable to that obtained in the control medium without the addition of a nitrogen source. These results are consistent with those of Chen et al [1] and Shih et al [25], whose studies on the growth of Fomes fomentarius and A. cinnamomea, respectively, revealed that certain essential amino acids could barely be synthesized from inorganic nitrogen sources, and hence that the synthesis of proteins and mycelial growth were inhibited.…”

“…It may thus be concluded that various carbon sources have different catabolic repression effects on the secondary cell metabolism. This phenomenon was observed in submerged cultures of many mushroom species [1,10,25]. Moreover, the study of Huang et al [8] showed that sucrose was one of the most preferable carbon sources for both EPS production and mycelial growth in the Chinese strain of H. erinaceus CZ-2.…”

“…In recent years there have been many reports on the optimization of cultivation conditions in submerged cultures of higher mushrooms in order to obtain a high level of mycelial biomass and EPS [1,10,31]. Traditional methods of optimizing the culture medium involve changing one independent parameter (e.g., a nutrient or pH) while keeping the others constant, so that it is possible to establish the effect of each parameter on the course of the process.…”

Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

“…F. fomentarius is commonly known as the Tinder Polypore, Hoof Fungus, Ice Man Fungus or Tinder Conk. Its basidioma is also known as the "Mudi" in Chinese, which has been used as a traditional Chinese medicine for many centuries in China for the treatment of various diseases (Chen et al 2008).…”

Exopolysaccharides Production and Characterization from Marine-Derived Penicillium commune KP942881.1 with Some Medical Potential Applications

“…There are several groups of microorganisms producing exopolysaccharides (EPSs) with different properties and applications, such as antitumor agents (Chen et al, 2008 andPeng et al, 2003), food packaging materials (Mc Neely et al, 1973), and wound dressing materials (Madla et al, 2005 andMethacanon et al, 2008). In particular, EPS produced by fungi to protect their cells against desiccation, phagocytosis and phage attack, toxic compounds, predation by protozoans, and osmotic stress, as well as in cellular recognition (Vuyst et al, 1999).…”