Optimization in the immobilization of penicillin G acylase by entrapment in xerogel particles with magnetic properties

Bernardino, Susana; Estrela, Nídia; Ochoa-Mendes, Vanessa; Fernandes, Pedro; Fonseca, Luís P.

doi:10.1007/s10971-011-2426-7

Cited by 23 publications

(10 citation statements)

References 55 publications

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“…This could be ascribed to leakage of enzyme from the gel, which has been shown to be particularly relevant for most hydrogels, namely, for calcium alginate supports [ 7 , 35 ]. The composition of the sol-gel used is likely to present a relatively low pore size, particularly when compared to hydrogels although some controversy exists on this matter on the effect of the nature of the sol-gel precursors on the pore size of the sol-gel particles [ 36 – 39 ]. Immobilization by binding to Amberlite or to Eupergit led to poorer results than those observed for entrapment methods safe for alginate.…”

Section: Resultsmentioning

confidence: 99%

Screening of Supports for the Immobilization of β-Glucosidase

et al. 2011

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A set of supports were screened for the immobilization of a partially purified extract of β-glucosidase from Aspergillus sp. These supports, namely, Eupergit, Amberlite, alginate, gelatin, polyvinyl alcohol- (PVA-) based matrices (Lentikats), and sol-gel, have proved effective for the implementation of some other enzyme-based processes. The initial criterion for selection of promising supports prior to further characterization relied on the retention of the catalytic activity following immobilization. Based on such criterion, where immobilization in sol-gel and in Lentikats outmatched the remaining approaches, those two systems were further characterized. Immobilization did not alter the pH/activity profile, whereas the temperature/activity profile was improved when sol-gel support was assayed. Both thermal and pH stability were improved as a result of immobilization. An increase in the apparent K M (Michaelis constant) was observed following immobilization, suggesting diffusion limitations.

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Section: Resultsmentioning

confidence: 99%

Screening of Supports for the Immobilization of β-Glucosidase

et al. 2011

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“…As indicated in the introduction, many methods have been developed for the manufacturing of magnetic nanoparticles; these include methods like co-precipitation [17], hydrothermal [18], microemulsion [19], thermal decomposition [20], sol-gel [21], laser pyrolysis [22], etc. After a preselection based on applicability level, we have reduced the proposed methods to the ones presented in Table 1, where a comparison on their characteristics is given.…”

Section: Process Selectionmentioning

confidence: 99%

Upscale Design, Process Development, and Economic Analysis of Industrial Plants for Nanomagnetic Particle Production for Environmental and Biomedical Use

et al. 2020

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Very few economical and process engineering studies have been made concerning the scale-up and implementation of nanomagnetic particle manufacturing into a full-scale plant, and determination of its viability. In this work we describe such a study for two types of industrial plants, one for manufacturing magnetic particles for applications in the environmental area, and the other for manufacturing nanomagnetic particles for applications in the biotechnology area; the two different applications are compared. The following methodology was followed: establish the manufacturing process for each application; determine the market demand of the product (magnetic nanoparticles) for both applications; determine the production capacity of each plant; engineer all the manufacturing process, determining all the process units and performing all the mass and energy balances for both plants; scale-up the main equipment; and determine the global economic impact and profitability. At the end both plants are found to be technologically and economically viable, the characteristics of the final products being, however, quite different, as well as the process engineering, economic analysis, and scale-up.

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“…HRP was encapsulated for hydrogen peroxide sensing; urease and acetylcholinesterase were used for sensing metal ions (95). As with hydrogels, it is possible to simultaneously entrap enzymes and magnetic particles for easier isolation of the xerogel microparticles (67,96).…”

Section: Sol-gelsmentioning

confidence: 99%

Enzyme Immobilization by Microencapsulation: Methods, Materials, and Technological Applications

Rother

Nidetzky

2014

Encyclopedia of Industrial Biotechnology

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Microencapsulation as a major technique of enzyme immobilization is reviewed. Fabrication of enzyme‐containing microcapsules is broadly categorized according to whether a semipermeable membrane coating or a porous polymeric network structure is primarily responsible for enclosure of enzymes within the internal microcapsule phase. Microcapsules are mostly spherical with diameters usually in the micro‐ to millimeter range and exhibit core‐shell structures of variable complexity. Liposomes and vesicles formed from amphiphilic (co)‐polymers (polymersomes) are widely used to prepare membrane‐coated microcapsules. Hydrogels, sol–gels and other organic–inorganic hybrid materials, or layer‐by‐layer structures made through controlled assembly of polyelectrolytes are used to prepare microcapsules composed of internal polymeric networks. Method combination where polymeric network microcapsules are coated with a suitable membrane to generate tailored core‐shell structures is also used for enzyme encapsulation. Advances in the material sciences contribute to the development of microcapsules with improved properties such as enhanced morphological stability, reduced enzyme leakage, and designed physicochemical permeability and enzyme biocompatibility. Soluble enzymes, but also enzyme aggregates and other insoluble enzyme preparations, are encapsulated. Multienzyme microcapsules are interesting small‐scale bioreactors for confined and even compartmentalized cascade biotransformation. Techniques of microcapsule preparation are described, and applications of microencapsulated enzymes in biotechnology and biomedicine are discussed.

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Optimization in the immobilization of penicillin G acylase by entrapment in xerogel particles with magnetic properties

Cited by 23 publications

References 55 publications

Screening of Supports for the Immobilization of β-Glucosidase

Screening of Supports for the Immobilization of β-Glucosidase

Upscale Design, Process Development, and Economic Analysis of Industrial Plants for Nanomagnetic Particle Production for Environmental and Biomedical Use

Enzyme Immobilization by Microencapsulation: Methods, Materials, and Technological Applications

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