Optimization, isolation, characterization and hepatoprotective effect of a novel pigment-protein complex (phycocyanin) producing microalga: Phormidium versicolorNCC-466 using response surface methodology

Gammoudi, Sana; Athmouni, Khaled; Nasri, Amèni; Diwani, Nouha; Grati, Ines; Belhaj, Dalel; Bouaziz‐Ketata, Hanen; Fki, Lotfi; Feki, Abdelfattah El; Ayadi, Habib

doi:10.1016/j.ijbiomac.2019.06.237

Cited by 20 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Prevention action against hepatotoxicity caused by cadmium. [117] Anti-inflammatory Spirulina sp. / Inhibits of albumin denaturation, anti-proteinase, hypotonicity-induced haemolysis and anti-lipoxygenase activities.…”

Section: Biotechnological Perspectives Of Pbpsmentioning

confidence: 99%

Phycobiliproteins: Structural aspects, functional characteristics, and biotechnological perspectives

Dagnino‐Leone

Figueroa

Castañeda

et al. 2022

Computational and Structural Biotechnology Journal

View full text Add to dashboard Cite

Section: Biotechnological Perspectives Of Pbpsmentioning

confidence: 99%

Phycobiliproteins: Structural aspects, functional characteristics, and biotechnological perspectives

Dagnino‐Leone

Figueroa

Castañeda

et al. 2022

Computational and Structural Biotechnology Journal

View full text Add to dashboard Cite

“…ASK22, Scenedesmus obliquus, , and Spirulina sp . In PBP research, the application of RSM has been focused on their extraction and purification. − Until now, there are few reports such as the works of Singh et al . and Mogany et al.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

et al. 2021

View full text Add to dashboard Cite

Phycobiliproteins (PBPs) are a group of brilliant pigment proteins found in cyanobacteria and red algae; their synthesis and accumulation depend on several factors such as the type of strain employed, nutrient concentration, light intensity, light regimes, and others. This study evaluates the effect of macronutrients (citrate buffer, NaNO 3 , K 2 HPO 4 , MgSO 4 , CaCl 2 , Na 2 CO 3 , and EDTA) and the concentration of trace metals in BG-11 media on the accumulation of PBPs in a thermotolerant strain of Oscillatoria sp. The strain was grown in BG-11 media at 28 °C with a light:dark cycle of 12:12 h at 100 μmol m –2 s –1 for 15 days, and the effect of nutrients was evaluated using a Plackett–Burman Design followed by optimization using a response surface methodology. Results from the concentration of trace metals show that it can be reduced up to half-strength in its initial concentration without affecting both biomass and PBPs. Results from the Plackett–Burman Design revealed that only NaNO 3 , Na 2 CO 3 , and K 2 HPO 4 show a significant increase in PBP production. Optimization employed a central Non-Factorial Response Surface Design with three levels and four factors (3 4 ) using NaNO 3 , Na 2 CO 3 , K 2 HPO 4 , and trace metals as variables, while the other components of BG-11 media (citrate buffer, MgSO 4 , CaCl 2 , and EDTA) were used in half of their initial concentration. Results from the optimization show that interaction between Na 2 CO 3 and K 2 HPO 4 highly increased PBPs’ concentration, with values of 15.21, 3.95, and 1.89 (% w/w), respectively. These results demonstrate that identifying and adjusting the concentration of critical nutrients can increase the concentration of PBPs up to two times for phycocyanin and allophycocyanin while four times for phycoerythrin. Finally, the reduction in non-key nutrients’ concentration will reduce the production costs of colorants at an industrial scale and increase the sustainability of the process.

show abstract

“…The maximum PC concentration was 67.45 mg/g at solidwater ratio 1/2, temperature 32.5 °C and pH 7.2. These parameters were used for the prevention of cadmium hepatoxicity [44]. PC from Spirulina platensis was produced in mixotrophic condition using RSM.…”

Section: The Effects Of Reactants On Phycocyanin (Pc) Concentrations By Using Response Surface Methodologymentioning

confidence: 99%

Enhancing Phycoerythrin and Phycocyanin Production from Porphyridium cruentum CCALA 415 in Synthetic Wastewater: The Application of Theoretical Methods on Microalgae

Onay

2021

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi

View full text Add to dashboard Cite

A stra t: Phycoerythrin (PE) and phycocyanin (PC) are florescent pigments. They have the colorant role in the industry. In this study, production of PE and PC from Porphyridium cruentum were investigated at the various conditions such as different concentrations of municipal wastewater, wavelengths and salicylic acid using Response Surface Methodology-Central Composite Design (RSM-CCD), regression analysis and rstool models. The maximum RSM predicted PE concentration was 29.5 mg/g biomass at 50 % of wastewater, 510 nm of wavelength and 10 µM of salicylic acid. On the other hand, maximum RSM predicted PC concentration was 6.9 mg/g biomass at 50% of wastewater, 680 nm and 40 µM of salicylic acid. According to the ANOVA results, the square effects of the three variables (X1, X2 and X3) were found to be significant for the phycocyanin concentration, while the wastewater and salicylic acid variables (X1 and X3) were found to be important in the Phycoerythrin concentration. In addition to this, the highest PE and PC concentrations were 27.648 and 5.7104 mg/g biomass, respectively, for 50 % of wastewater, 512.5 nm and 47.0833 µM of salicylic acid according to rstool model. In con clusion, the variables such as wastewater, wavelength and salicylic acid can be used for the highest PE and PC concentration by means of RSM-CCD and rstool models and these variables may contribute to the industrial production of the two pigments.

show abstract

Optimization, isolation, characterization and hepatoprotective effect of a novel pigment-protein complex (phycocyanin) producing microalga: Phormidium versicolorNCC-466 using response surface methodology

Cited by 20 publications

References 53 publications

Phycobiliproteins: Structural aspects, functional characteristics, and biotechnological perspectives

Phycobiliproteins: Structural aspects, functional characteristics, and biotechnological perspectives

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

Enhancing Phycoerythrin and Phycocyanin Production from Porphyridium cruentum CCALA 415 in Synthetic Wastewater: The Application of Theoretical Methods on Microalgae

Contact Info

Product

Resources

About