Optimization of 3D bioprinting of human neuroblastoma cells using sodium alginate hydrogel

Lewicki, Jakub; Bergman, Joost; Kerins, Caoimhe; Hermanson, Ola

doi:10.1016/j.bprint.2019.e00053

Cited by 50 publications

(35 citation statements)

References 51 publications

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“…The FRESH method proves that high resolution of neuroblastoma 3D structures can be obtained using low-viscosity liquids in a supporting bath of gelatin. Neuroblastoma cells can be successfully grown in cellulose and alginate-based hydrogels, demonstrating the applicability of FRESH bioprinting for the generation of microsized 3D neuroblastoma tumors (74). The same type of hydrogel has been explored in the immunology field as well showing the changes in immunophenotype profiles in neuroblastoma cells surrounded by biomimetic ECM (75).…”

Section: (Bio)printing Neuroblastoma 3d Modelsmentioning

confidence: 96%

Emerging Neuroblastoma 3D In Vitro Models for Pre-Clinical Assessments

Corallo

Frabetti²,

Candini³

et al. 2020

Front. Immunol.

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The potential of tumor three-dimensional (3D) in vitro models for the validation of existing or novel anti-cancer therapies has been largely recognized. During the last decade, diverse in vitro 3D cell systems have been proposed as a bridging link between two-dimensional (2D) cell cultures and in vivo animal models, both considered gold standards in pre-clinical settings. The latest awareness about the power of tailored therapies and cell-based therapies in eradicating tumor cells raises the need for versatile 3D cell culture systems through which we might rapidly understand the specificity of promising anti-cancer approaches. Yet, a faithful reproduction of the complex tumor microenvironment is demanding as it implies a suitable organization of several cell types and extracellular matrix components. The proposed 3D tumor models discussed here are expected to offer the required structural complexity while also assuring cost-effectiveness during pre-selection of the most promising therapies. As neuroblastoma is an extremely heterogenous extracranial solid tumor, translation from 2D cultures into innovative 3D in vitro systems is particularly challenging. In recent years, the number of 3D in vitro models mimicking native neuroblastoma tumors has been rapidly increasing. However, in vitro platforms that efficiently sustain patient-derived tumor cell growth, thus allowing comprehensive drug discovery studies on tailored therapies, are still lacking. In this review, the latest neuroblastoma 3D in vitro models are presented and their applicability for a more accurate prediction of therapy outcomes is discussed.

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Section: (Bio)printing Neuroblastoma 3d Modelsmentioning

confidence: 96%

Emerging Neuroblastoma 3D In Vitro Models for Pre-Clinical Assessments

Corallo

Frabetti²,

Candini³

et al. 2020

Front. Immunol.

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“…A similar approach, in this case described as 'FRESH' (freeform reversible embedding of suspended hydrogels), was adopted by the group of Hermanson, who reported a moderately low concentration alginate bioink (2.0% wt/vol) for 3D bioprinting of human neuroblastoma cells. 78 A gelatin slurry was used for physical support during printing and CaCl 2 (100 mM) was used as an ionic cross-linker for the alginate chains. The gelatin sacrificial template was then removed by incubating the scaffolds at 37 1C.…”

Section: Neural Scaffoldsmentioning

confidence: 99%

“…38 We have described various examples of bioink formulations for neural scaffolds in which alginate was the only component. 35,[73][74][75][76][77][78] Such scaffolds were fabricated using alginate concentrations between 0.5-3.0% wt/vol, with the best results in terms of cell growth at low alginate concentrations. 75,76 The use of gelatin sacrificial templates allowed materials with higher mechanical stability and better shape fidelity.…”

Section: Design Of Alginate-based Bioink Formulations For Specific Apmentioning

confidence: 99%

“…75,76 The use of gelatin sacrificial templates allowed materials with higher mechanical stability and better shape fidelity. 77,78 The combination of alginate with other polysaccharides is a way of avoiding the use of sacrificial templates. Examples of multicomponent bioinks were obtained by combining alginate with agarose (1.5% wt/vol) 87,88 or hyaluronic acid (0.25% wt/vol), 76,149,150 which provided the necessary mechanical support to the 3D printed structures (Fig.…”

Section: Design Of Alginate-based Bioink Formulations For Specific Apmentioning

confidence: 99%

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Multicomponent polysaccharide alginate-based bioinks

2020

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“…Extrusion bioprinting involves the deposition of biomolecules and cells that are encapsulated inside a flowable hydrogel matrix, resulting in a highly bioactive structure. Optimization of printing speed, pressure, and temperature is required for any minute changes in the bioink, such as the concentration of encapsulated cells [44]. Bioinks loaded with polysaccharides and proteins (e.g., collagen, alginate) can show very different values of viscosity.…”

Section: Process and Materialsmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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Optimization of 3D bioprinting of human neuroblastoma cells using sodium alginate hydrogel

Cited by 50 publications

References 51 publications

Emerging Neuroblastoma 3D In Vitro Models for Pre-Clinical Assessments

Emerging Neuroblastoma 3D In Vitro Models for Pre-Clinical Assessments

Multicomponent polysaccharide alginate-based bioinks

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

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