Optimization of a GDNF production method based on Semliki Forest virus vector

Torres-Ortega, Pablo Vicente; Smerdou, Cristian; Ansorena, Eduardo; Ballesteros-Briones, María Cristina; Martišová, Eva; Garbayo, Elisa; Blanco-Prieto, María J.

doi:10.1016/j.ejps.2021.105726

Cited by 1 publication

(2 citation statements)

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“…Human GDNF was expressed and purified from BHK-21 cells using a Semliki Forest virus (SFV) expressing vector as described before . GDNF-loaded NPs were prepared by solvent extraction/evaporation method using the Total Recirculation One-Machine System (TROMS).…”

Section: Experimental Section/methodsmentioning

confidence: 99%

“…Human GDNF was expressed and purified from BHK-21 cells using a Semliki Forest virus (SFV) expressing vector as described before. 34 GDNF-loaded NPs were prepared by solvent extraction/evaporation method using the Total Recirculation One-Machine System (TROMS). Briefly, the organic solution composed of methylene chloride/acetone (2 mL, 3:1) and Resomer RG 503H poly(lactic- co -glycolic acid) (PLGA) (50 mg, M w : 24,000–38,000 Da) was injected through a needle with an inner gauge diameter of 0.17 mm at a ratio of 35 mL/min into the inner aqueous phase (150 μL).…”

Section: Experimental Section/methodsmentioning

confidence: 99%

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Encapsulation of MSCs and GDNF in an Injectable Nanoreinforced Supramolecular Hydrogel for Brain Tissue Engineering

et al. 2022

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The co-administration of glial cell line-derived neurotrophic factor (GDNF) and mesenchymal stem cells (MSCs) in hydrogels (HGs) has emerged as a powerful strategy to enhance the efficient integration of transplanted cells in Parkinson's disease (PD). This strategy could be improved by controlling the cellular microenvironment and biomolecule release and better mimicking the complex properties of the brain tissue. Here, we develop and characterize a drug delivery system for brain repair where MSCs and GDNF are included in a nanoparticle-modified supramolecular guest−host HA HG. In this system, the nanoparticles act as both carriers for the GDNF and active physical crosslinkers of the HG. The multifunctional HG is mechanically compatible with brain tissue and easily injectable. It also protects GDNF from degradation and achieves its controlled release over time. The cytocompatibility studies show that the developed biomaterial provides a friendly environment for MSCs and presents good compatibility with PC12 cells. Finally, using RNA-sequencing (RNA-seq), we investigated how the three-dimensional (3D) environment, provided by the nanostructured HG, impacted the encapsulated cells. The transcriptome analysis supports the beneficial effect of including MSCs in the nanoreinforced HG. An enhancement in the anti-inflammatory effect of MSCs was observed, as well as a differentiation of the MSCs toward a neuron-like cell type. In summary, the suitable strength, excellent selfhealing properties, good biocompatibility, and ability to boost MSC regenerative potential make this nanoreinforced HG a good candidate for drug and cell administration to the brain.

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Section: Experimental Section/methodsmentioning

confidence: 99%

Section: Experimental Section/methodsmentioning

confidence: 99%