Optimization of a laccase-mediator system with natural redox-mediating compounds for pesticide removal

Kupski, Larine; Salcedo, Gabriela M.; Caldas, Sergiane Souza; Souza, Taiana Denardi de; Furlong, Eliana Badiale; Prímel, Ednei Gilberto

doi:10.1007/s11356-018-4010-y

Cited by 53 publications

(23 citation statements)

References 45 publications

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“…Due to enzymatic oxidation of ABTS can produce color changes, the absorbance was monitored at 420 nm using a TU-1900 spectrophotometer. The measurements were performed out every 0.05 absorbance increment (Kupski et al 2019). The amount of Lac required to oxidize 1 μmol of ABTS in 1 min is defined as one unit of Lac (U).…”

Section: Activity Assay Of Lacmentioning

confidence: 99%

Degradation of octylphenol polyethoxylates with a long ethoxylate chain using the laccase-mediated systems

Wang

Lou

Yuan

et al. 2021

Environ Sci Pollut Res

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Alkylphenol polyethoxylates (APEO n ) are the second-largest category of commercial nonionic surfactants, which are difficult to degrade naturally in the environment. This study examined the degradation of octylphenol polyethoxylate (OPEO n ) by laccase and its laccase-mediated systems. The results showed that OPEO n was poorly degraded by laccase alone. 2, 2′-azino-bis [3ethylbenzothiazoline-6-sulphonic acid] (ABTS), 1-hydroxybenzotriazole (HBT), and 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) were selected as the redox mediators. Experimental results also indicated that 52.4% of the initial OPEO n amount was degraded by laccase in the presence of TEMPO. The degradation efficiency was analyzed using high-performance liquid chromatography. Furthermore, the structural characteristics of the degradation products were measured using matrix-assisted laser desorption/ionization-time of flight mass spectrometry and nuclear magnetic resonance spectroscopy, and it could be found that the laccase-TEMPO system could gradually shorten the ethoxylate chain by oxidizing the primary hydroxyl group of OPEO n , thereby degrading the OPEO n of the macromolecule into small molecules. The maximum of the ion peak distributions of OPEO n decreased from n = 8 finally down to 3. The novel enzymatic system introduced by this study will become a promising alternative method for high-efficiency APEO n conversion and had great potential value in wastewater treatment.

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Section: Activity Assay Of Lacmentioning

confidence: 99%

Degradation of octylphenol polyethoxylates with a long ethoxylate chain using the laccase-mediated systems

Wang

Lou

Yuan

et al. 2021

Environ Sci Pollut Res

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“…These new compounds may stabilize, or undergo rearrangement and generate new products, which can also act as oxidizing agents. When combined with electron shuttles-so called mediators-laccases more effectively oxidize nonphenolic substrates via different mechanisms, such as electron transfer or radical hydrogen atom transfer (Christopher (Kupski et al 2019). Detoxification methods using laccases or laccase-mediator-systems (LMS) can be performed using free or immobilized enzymes, with or without oxygen.…”

Section: Laccases (Aa1)mentioning

confidence: 99%

Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications

Tramontina

Brenelli

Sodré

et al. 2020

World J Microbiol Biotechnol

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The physicochemical pretreatment is an important step to reduce biomass recalcitrance and facilitate further processing of plant lignocellulose into bioproducts. This process results in soluble and insoluble biomass fractions, and both may contain by-products that inhibit enzymatic biocatalysts and microbial fermentation. These fermentation inhibitory compounds (ICs) are produced during the degradation of lignin and sugars, resulting in phenolic and furanic compounds, and carboxylic acids. Therefore, detoxification steps may be required to improve lignocellulose conversion by microoganisms. Several physical and chemical methods, such as neutralization, use of activated charcoal and organic solvents, have been developed and recommended for removal of ICs. However, biological processes, especially enzyme-based, have been shown to efficiently remove ICs with the advantage of minimizing environmental issues since they are biogenic catalysts and used in low quantities. This review focuses on describing several enzymatic approaches to promote detoxification of lignocellulosic hydrolysates and improve the performance of microbial fermentation for the generation of bioproducts. Novel strategies using classical carbohydrate active enzymes (CAZymes), such as laccases (AA1) and peroxidases (AA2), as well as more advanced strategies using prooxidant, antioxidant and detoxification enzymes (dubbed as PADs), i.e. superoxide dismutases, are discussed as perspectives in the field.

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“…The laccase-redox-mediator system is one of the most-investigated enzyme reactions in ligninolytic enzyme systems [ 4 , 5 ]. This system has been used to remove many emerging contaminants that are difficult to decompose, such as dye-based industrial pollutants and endocrine-disrupting compounds [ 6 , 7 , 8 , 9 ]. Moreover, among the lignocellulytic enzymes, cellulases have been found to have significant potential for industrial applications, especially in the food, chemical, detergent, cosmetics, and pulp and paper sectors, among others [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Fungus Enzymes in Spent Mushroom Composts from Edible Mushroom Cultivation for Phthalate Removal

Chang

Yang

2021

Microorganisms

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Spent mushroom composts (SMCs) are waste products of mushroom cultivation. The handling of large amounts of SMCs has become an important environmental issue. Phthalates are plasticizers which are widely distributed in the environment and urban wastewater, and cannot be effectively removed by conventional wastewater treatment methods. In this study, SMCs are tested for their ability to remove phthalates, including benzyl butyl phthalate (BBP), di-n-butyl phthalate (DBP), and diethyl phthalate (DEP). Batch experiments reveal that BBP, DBP, and DEP can be degraded by the SMC enzyme extracts of four edible mushrooms: Pleurotus eryngii, Pleurotus djamor, Pleurotus ostreatus, and Auricularia polytricha. Potential fungus enzymes associated with BBP, DBP, and DEP degradation in SMCs (i.e., esterases, oxygenases, and oxidases/dehydrogenases) are uncovered by metaproteomic analysis using mass spectrometry. Bioreactor experiments indicate that the direct application of SMCs can remove BBP, DBP, and DEP from wastewater, through adsorption and biodegradation. The results of this study extend the application of white-rot fungi without laccases (e.g., Auricularia sp.) for the removal of organic pollutants which are not degraded by laccases. The application of SMCs for phthalate removal can be developed into a mycoremediation-based green and sustainable technology.

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Optimization of a laccase-mediator system with natural redox-mediating compounds for pesticide removal

Cited by 53 publications

References 45 publications

Degradation of octylphenol polyethoxylates with a long ethoxylate chain using the laccase-mediated systems

Degradation of octylphenol polyethoxylates with a long ethoxylate chain using the laccase-mediated systems

Enzymatic removal of inhibitory compounds from lignocellulosic hydrolysates for biomass to bioproducts applications

Application of Fungus Enzymes in Spent Mushroom Composts from Edible Mushroom Cultivation for Phthalate Removal

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